FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-119-4-8-2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-119-4-8-2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27438
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA447916.324076098841022No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAT337912.31503753917924No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATTCCTCTGT17646.429040017493986No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATTCCTC15375.6017202420001455No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA8923.2509658138348274No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAT8813.21087542823821No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATTCCTC5481.9972301188133246No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATTCCTCTG4711.7165974196369997No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA4691.7093082586194328No Hit
CAACACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTATTCCT3951.4396093009694584No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATTCCT3931.4323201399518914No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA3901.4213863984255413No Hit
CAACACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC3861.4068080763904074No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA3711.3521393687586558No Hit
CAACACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATTCCT3241.1808440848458341No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA3201.1662657628107005No Hit
CAACACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA3011.0970187331438153No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT2961.078795830599898No Hit
CAACACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATTCCTCT2600.9475909322836942No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAT2280.8309643560026241No Hit
CAACACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTATT2220.8090968729499234No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA1960.714337779721554No Hit
CAACACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATTCCTC1730.6305124280195349No Hit
CAACACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATTC1690.6159341059844012No Hit
CAACACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTATT1690.6159341059844012No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAT1560.5685545593702165No Hit
CAACACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATTCCT1520.5539762373350827No Hit
CAACACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTATTCCTC1360.49566294919454773No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA1350.49201836868576426No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA1320.4810846271594139No Hit
CAACACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTATTCC1290.4701508856330637No Hit
CAACACACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTT1210.4409942415627961No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG1200.43734966105401263No Hit
CAACACACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTATTCC1170.4264159195276624No Hit
CAACACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTATT1150.4191267585100955No Hit
CAACACACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTATTC1060.3863255339310446No Hit
CAACACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC1040.37903637291347764No Hit
CAACACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTATT1020.37174721189591076No Hit
CAACACACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAT1010.36810263138712734No Hit
CAACACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATTCCTCT930.3389459873168598No Hit
CAACACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAT900.3280122457905095No Hit
CAACACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT890.32436766528172606No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTATTCCTC890.32436766528172606No Hit
CAACACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTATTCCTC890.32436766528172606No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA870.3170785042641592No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA860.3134339237553757No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA860.3134339237553757No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC760.27698811866754136No Hit
CAACACACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTT730.26605437714119107No Hit
CAACACACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTATTCC710.25876521612362413No Hit
CAACACACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTATTCC650.23689773307092354No Hit
CAACACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTATTCC640.2332531525621401No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA630.22960857205335666No Hit
CAACACACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGCC620.22596399154457322No Hit
CAACACACTGGGATTAGATACCCCTTCTAGAGGAGCCTGTTCTATTCCTC620.22596399154457322No Hit
CAACACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTATT600.21867483052700631No Hit
CAACACACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTATTCCT590.21503025001822293No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAT590.21503025001822293No Hit
CAACACACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTATT590.21503025001822293No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA570.20774108900065602No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA560.20409650849187258No Hit
CAACACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTATTCCTC560.20409650849187258No Hit
CAACACACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTAT550.20045192798308914No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA540.19680734747430573No Hit
CAACACACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCT520.18951818645673882No Hit
CAACACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTATTCCTC510.18587360594795538No Hit
CAACACACTGGGATTAGATACCCCTGACGCTGCTAGAGGAGCCTGTTCTA510.18587360594795538No Hit
CAACACACTGGGATTAGATACCCCCGTGGACTAGAGGAGCCTGTTCTATT510.18587360594795538No Hit
CAACACACTGGGATTAGATACCCCGAGATGCCTAAGCTAGAGGAGCCTGT510.18587360594795538No Hit
CAACACACTGGGATTAGATACCCCTGTAGTTCTAGAGGAGCCTGTTCTAT500.18222902543917197No Hit
CAACACACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTATTCC500.18222902543917197No Hit
CAACACACTGGGATTAGATACCCCCAGCCCTATGAAAAGGGTGCTAGAGG500.18222902543917197No Hit
CAACACACTGGGATTAGATACCCCTCCAGCCCACGAGACCTAGAGGAGCC490.1785844449303885No Hit
CAACACACTGGGATTAGATACCCCCGCAACCTAGAGGAGCCTGTTCTATT490.1785844449303885No Hit
CAACACACTGGGATTAGATACCCCAGTCCTAGAGGAGCCTGTTCTATTCC470.17129528391282164No Hit
CAACACACTGGGATTAGATACCCCTGACTGCCGACTAGAGGAGCCTGTTC460.16765070340403818No Hit
CAACACACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTATTCC460.16765070340403818No Hit
CAACACACTGGGATTAGATACCCCCCACGAGACCTAGAGGAGCCTGTTCT460.16765070340403818No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCCCTAGAGGAGCCTGTTCT440.16036154238647132No Hit
CAACACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTATTCC430.15671696187768785No Hit
CAACACACTGGGATTAGATACCCCAGCTAGAGGAGCCTGTTCTATTCCTC430.15671696187768785No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA400.14578322035133756No Hit
CAACACACTGGGATTAGATACCCCCGGACCTAGAGGAGCCTGTTCTATTC390.14213863984255412No Hit
CAACACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTATTCCT390.14213863984255412No Hit
CAACACACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTATTC390.14213863984255412No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA380.13849405933377068No Hit
CAACACACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTATTCCT360.1312048983162038No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA350.1275603178074204No Hit
CAACACACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGAG340.12391573729863693No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT340.12391573729863693No Hit
CAACACACTGGGATTAGATACCCCAAGCCCACGAGATCTAGAGGAGCCTG320.11662657628107005No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTATT320.11662657628107005No Hit
CAACACGCTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAT300.10933741526350316No Hit
CAACACACTGGGATTAGATACCCCTGTCAGACTAGAGGAGCCTGTTCTAT290.10569283475471973No Hit
CAACACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT280.10204825424593629No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCAGAACCTAGAGGAGCCT280.10204825424593629No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGATT157.1648756E-6294.987248
CGCTGGG255.18412E-10294.98726
GCTGGGA255.18412E-10294.98727
CACTGGG26550.0294.98726
CACCCTG108.421162E-4294.98724
TGGGATA108.421162E-4294.98729
CTGGGAT26900.0294.438938
ACTGGGA26600.0294.432747
TGGGATT26800.0293.886549
ACACTGG26900.0293.342325
CACACTG27250.0290.11594
ACACACT29000.0273.11753
ACGCTGG301.540684E-9245.82275
CACGCTG301.540684E-9245.82274
CAACACA33700.0235.464581
AACACAC33900.0234.075422
TGGATTA202.2583687E-5221.240429
CACTGGA359.89141E-7168.564136
ACACGCT451.7400453E-8163.881793
ACTGGAT301.13716305E-4147.493627
Produced by FastQC (version 0.11.5)