FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-118-5-2-6-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-118-5-2-6-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26387
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA875333.171637548792965No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA20857.90161822109372No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG14635.544396862091181No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGGAATCGCT13395.074468488270739No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA9313.5282525486034793No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA7712.9218933565771024No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGGAATC6562.486072687308144No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT4741.7963391063781406No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA3931.4893697654147875No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG3831.451472315913139No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA3281.243036343654072No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA3051.1558722098002805No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGGAATCGC2831.0724978208966536No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGGAAT2661.008072156743851No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGGAATCG2620.9929131769431917No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGGAATC2230.8451131238867624No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA2230.8451131238867624No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGGAAT1990.754159245082806No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAG1850.7011028157804979No Hit
GCCACAACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAGG1820.6897335809300034No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA1780.674574601129344No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA1680.6366771516276954No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA1620.6139386819267063No Hit
GCCACAACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGGA1500.5684617425247281No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA1420.5381437829234093No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA1390.5267745480729147No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG1250.47371811877060677No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1190.4509796490696176No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1180.44718990411945275No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA1160.43961041421912306No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAG1150.4358206692689582No Hit
GCCACAACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGGAAT990.3751847500663205No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA960.36381551521582595No Hit
GCCACAACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC870.3297078106643423No Hit
GCCACAACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGGAA830.3145488308636829No Hit
GCCACAACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGGAATC820.310759085913518No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA810.30696934096335315No Hit
GCCACAACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGGAATCG800.30317959601318833No Hit
GCCACAACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTAGGA710.2690718914617046No Hit
GCCACAACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTAGGA630.2387539318603858No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC620.23496418691022095No Hit
GCCACAACTGGGATTAGATACCCCATGTGTATAGAGGAGCCTGTTCTAGG580.21980520710956153No Hit
GCCACAACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAGGAA570.21601546215939668No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT570.21601546215939668No Hit
GCCACAACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGGAATC560.2122257172092318No Hit
GCCACAACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGGAATC560.2122257172092318No Hit
GCCACAACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA540.2046462273089021No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGGAAT500.1894872475082427No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGG480.18190775760791297No Hit
GCCACAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAGG460.17432826770758328No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG450.17053852275741843No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG440.16674877780725356No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA410.155379542956759No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA410.155379542956759No Hit
GCCACAACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAG390.1478000530564293No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATG390.1478000530564293No Hit
GCCACAACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT380.14401030810626445No Hit
GCCACAACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGGAATC380.14401030810626445No Hit
GCCACAACTGGGATTAGATACCCCATAATCCTAGAGGAGCCTGTTCTAGG370.1402205631560996No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGG360.13643081820593475No Hit
GCCACAACTGGGATTAGATACCCCGCCAACTAGAGGAGCCTGTTCTAGGA360.13643081820593475No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG340.12885132830560503No Hit
GCCACAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA330.12506158335544018No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA310.11748209345511047No Hit
GCCACAACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAGG310.11748209345511047No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT300.11369234850494561No Hit
GCCACAACTGGGATTAGATACCCCTGTCAGACTAGAGGAGCCTGTTCTAG300.11369234850494561No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA290.10990260355478076No Hit
GCCACAACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGGAA290.10990260355478076No Hit
GCCACAACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGG290.10990260355478076No Hit
GCCACAACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA290.10990260355478076No Hit
GCCACAACTGGGATTAGATACCCCGCAACCTAGAGGAGCCTGTTCTAGGA280.1061128586046159No Hit
GCCACAACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTAG280.1061128586046159No Hit
GCCACAACTGGGATTAGATACCCCACAACCTAGAGGAGCCTGTTCTAGGA270.10232311365445106No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA270.10232311365445106No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGTT108.422778E-4294.94698
TGGGTTA108.422778E-4294.94699
ACTGGGT108.422778E-4294.94697
AACTGGG25750.0294.946876
CTGGATT303.6379788E-12294.946878
CAACTGG26150.0293.818975
CTGGGAT25800.0293.803658
TGGGATT25650.0293.796979
ACTGGGA25850.0292.66497
GCCACAA26550.0291.05871
CCACAAC26650.0289.413182
CACAACT26750.0287.779943
ACAACTG27050.0284.043124
TGGGATA202.2586184E-5221.210169
CACCCGA150.0028355122196.63126295
TGGATTA457.4578566E-11196.631249
ACTGGAT457.4578566E-11196.631247
AACTGGA501.5643309E-10176.968126
CCGCTAT201.8087393E-459.214120-24
CTCTATG201.8087393E-459.214120-24
Produced by FastQC (version 0.11.5)