FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-117-5-7-5-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-117-5-7-5-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25829
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG853933.05973905300244No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAAG18937.328971311316737No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG18727.2476673506523674No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAAGTCTCCTGT9413.6431917611986524No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC8573.3179759185411744No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG8163.1592396143869292No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAAGTCTCC4901.8970924155019553No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT4661.8041736033141045No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG4001.5486468697975144No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAAG3131.211816175616555No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAAGTCTCCT2991.157613535173642No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG2591.0027488481938907No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAAG2360.9137016531805335No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAAGTCTC2310.8943435673080646No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAA2260.8749854814355957No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG2250.8711138642611018No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT2190.8478841612141392No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAAGTCTC2030.7859382864222386No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAAGTC1790.6930194742343877No Hit
AAGCGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAAGTCT1790.6930194742343877No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAA1750.6775330055364126No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT1730.669789771187425No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG1530.5923574276975493No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAAGTCTCC1520.5884858105230555No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG1440.5575128731271052No Hit
AAGCGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA1360.5265399357311549No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG1340.5187967013821673No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG1320.5110534670331798No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAAGTCTC1260.4878237639862171No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAAGTCTCCTG1240.48008052963722947No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAA1150.44523597506678536No Hit
AAGCGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAAGTCT960.37167524875140345No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG920.3561887800534283No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAAG840.32521584265747805No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAAGT820.31747260830849044No Hit
AAGCGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAAGT820.31747260830849044No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAAGT770.29811452243602155No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG750.29037128808703394No Hit
AAGCGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAAGT740.28649967091254014No Hit
AAGCGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAAGT730.2826280537380464No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAA710.2748848193890588No Hit
AAGCGACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGGT700.27101320221456504No Hit
AAGCGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAAGT700.27101320221456504No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT670.25939835069108363No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG660.2555267335165899No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG640.24778349916760228No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAAGT620.24004026481861473No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT510.19745247589918308No Hit
AAGCGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG510.19745247589918308No Hit
AAGCGACTGGGATTAGATACCCCTGTAGCCCTAGAGGAGCCTGTTCTAAG500.1935808587246893No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAAGT490.1897092415501955No Hit
AAGCGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAAGTCTCC490.1897092415501955No Hit
AAGCGACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAAGTCTCCT480.18583762437570173No Hit
AAGCGACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAAGTCTCC470.18196600720120795No Hit
AAGCGACTGGGATTAGATACCCCTGTGCTAGAGGAGCCTGTTCTAAGTCT460.17809439002671415No Hit
AAGCGACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAAGTCTC440.17035115567772657No Hit
AAGCGACTGGGATTAGATACCCCTGACCTGCCGCTAGAGGAGCCTGTTCT440.17035115567772657No Hit
AAGCGACTGGGATTAGATACCCCTGACGCTGCTAGAGGAGCCTGTTCTAA430.1664795385032328No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCAGAACCTAGAGGAGCCTG420.16260792132873902No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC420.16260792132873902No Hit
AAGCGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAAGTCTCC410.15873630415424522No Hit
AAGCGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAAGTCTC410.15873630415424522No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACAAGACCTAGAGGAGCCTGT410.15873630415424522No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTAA410.15873630415424522No Hit
AAGCGACTGGGATTAGATACCCCCGGACCTAGAGGAGCCTGTTCTAAGTC370.14324983545627007No Hit
AAGCGACTGGGATTAGATACCCCTACCCTAGAGGAGCCTGTTCTAAGTCT370.14324983545627007No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT370.14324983545627007No Hit
AAGCGACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAA360.1393782182817763No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG350.13550660110728252No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCTA350.13550660110728252No Hit
AAGCGACTGGGATTAGATACCCCATACGACCCACTAGAGGAGCCTGTTCT350.13550660110728252No Hit
AAGCGACTGGGATTAGATACCCCACACTAGAGGAGCCTGTTCTAAGTCTC340.13163498393278872No Hit
AAGCGACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGCCT330.12776336675829494No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTGTT330.12776336675829494No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG320.12389174958380114No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG320.12389174958380114No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAAGTCTCC320.12389174958380114No Hit
AAGCGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAAGTCTCC320.12389174958380114No Hit
AAGCGACTGGGATTAGATACCCCCACGAACCTAGAGGAGCCTGTTCTAAG310.12002013240930737No Hit
AAGCGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAAGTCTCC310.12002013240930737No Hit
AAGCGACTGGGATTAGATACCCCTGACGCATAGAGGAGCCTGTTCTAAGT310.12002013240930737No Hit
AAGCGACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAAG300.11614851523481359No Hit
AAGCGACTGGGATTAGATACCCCCGAGCCCACGAGACCTAGAGGAGCCTG270.10453366371133221No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTAAG270.10453366371133221No Hit
AAGCGACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTTCT270.10453366371133221No Hit
AAGCGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT260.10066204653683844No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGG206.06924E-8295.03
CTGGGAT25300.0295.07
CTGGATT108.417216E-4295.07
CGGGATT206.06924E-8295.07
ACGGGAT206.06924E-8295.06
CGACGGG206.06924E-8295.04
TGGGATT25250.0295.08
ACTGGAT108.417216E-4295.06
AGCGACG206.06924E-8295.02
CGACTGG25450.0295.04
AGCGACT25650.0294.424932
GCGACTG25500.0294.421573
GACTGGG25400.0294.41935
ACTGGGA25400.0294.41936
AAGCGAC25900.0293.8611
GGGATTA25400.0292.67729
GACGGGA251.8469837E-7236.05
GCGACTA150.0028336423196.666663
GACTGGA150.0028336423196.666665
TAGGATT200.006699835147.58
Produced by FastQC (version 0.11.5)