FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-116-5-1-3-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-116-5-1-3-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26046
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC804730.89533901558781No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT19027.302464869845658No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA14815.686093833986025No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGGAATCGC12734.887506718881978No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT7682.94862934807648No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT7592.9140750979037087No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGGAAT7062.7105889579973894No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA4911.8851263149811872No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT4811.846732703678108No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGGAATCG3521.3514551178683867No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC3251.247792367350073No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGGAA2961.1364508945711433No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT2921.1210934500499117No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT2771.063503033095293No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGGAA2681.0289487829225217No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA2651.017430699531598No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGGAAT2520.9675190048375951No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT2290.8792136988405129No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC1910.7333179758888121No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC1640.6296552253704983No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT1590.6104584197189588No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT1540.5912616140674192No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1520.5835828918068033No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT1490.5720648084158796No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT1470.5643860861552638No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGGAATC1370.5259924748521846No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAG1350.5183137525915688No Hit
ATCGGTTACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT1280.4914382246794134No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC1110.42616908546417875No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT1090.4184903632035629No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT1080.414651002073255No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT1080.414651002073255No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGG1050.4031329186823312No Hit
ATCGGTTACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTA890.3417031405974046No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT770.2956308070337096No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT720.27643400138217No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAGG700.26875527912155417No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT680.26107655686093834No Hit
ATCGGTTACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGGAAT660.2533978346003225No Hit
ATCGGTTACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC660.2533978346003225No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG600.23036166781847503No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA580.22268294555785917No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGGAA560.21500422329724334No Hit
ATCGGTTACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTA540.2073255010366275No Hit
ATCGGTTACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGGA530.20348613990631959No Hit
ATCGGTTACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAG530.20348613990631959No Hit
ATCGGTTACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGGA510.19580741764570375No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTAGG510.19580741764570375No Hit
ATCGGTTACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGGAATC490.18812869538508792No Hit
ATCGGTTACTGGGATTAGATACCCCTTCTAGAGGAGCCTGTTCTAGGAAT490.18812869538508792No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATACACATTACGCTAGAGGAGC490.18812869538508792No Hit
ATCGGTTACTGGGATTAGATACCCCGCACGGACCTAGAGGAGCCTGTTCT480.18428933425478No Hit
ATCGGTTACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAGGA480.18428933425478No Hit
ATCGGTTACTGGGATTAGATACCCCACGGGACCTAGAGGAGCCTGTTCTA460.17661061199416417No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAG450.17277125086385625No Hit
ATCGGTTACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGGAAT430.16509252860324042No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAG400.15357444521231667No Hit
ATCGGTTACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA400.15357444521231667No Hit
ATCGGTTACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA390.14973508408200875No Hit
ATCGGTTACTGGGATTAGATACCCCCTGTAGTCCCTAGAGGAGCCTGTTC380.14589572295170083No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCT380.14589572295170083No Hit
ATCGGTTACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGGA370.14205636182139292No Hit
ATCGGTTACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTT360.138217000691085No Hit
ATCGGTTACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT360.138217000691085No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG350.13437763956077708No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGATCTAGAGGAGCCT340.13053827843046917No Hit
ATCGGTTACTGGGATTAGATACCCCACGTCTAGAGGAGCCTGTTCTAGGA330.12669891730016125No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCACGACCTAGAGGAGCCTG330.12669891730016125No Hit
ATCGGTTACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGGA320.12285955616985335No Hit
ATCGGTTACTGGGATTAGATACCCCTGTGTATAAGAGACAGCCACGTCTA320.12285955616985335No Hit
ATCGGTTACTGGGATTAGATACCCCGATCCCACGAGACCTAGAGGAGCCT320.12285955616985335No Hit
ATCGGTTACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA320.12285955616985335No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGACTAGAGGAGC310.11902019503954543No Hit
ATCGGTTACTGGGATTAGATACCCCCGAGCCCACGAGACCTAGAGGAGCC310.11902019503954543No Hit
ATCGGTTACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTA310.11902019503954543No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAAT310.11902019503954543No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGGCCTAGAGGAGCCTGTTCTAG290.11134147277892958No Hit
ATCGGTTACTGGGATTAGATACCCCTGACCTGGAACTAGAGGAGCCTGTT280.10750211164862167No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGAT280.10750211164862167No Hit
ATCGGTTACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGGAAT280.10750211164862167No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC280.10750211164862167No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAAT270.10366275051831375No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGTCTAGAGGAGCCTGTTCTAG270.10366275051831375No Hit
ATCGGTTACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAGGA270.10366275051831375No Hit
ATCGGTTACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTAGGA270.10366275051831375No Hit
ATCGGTTACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAGGA270.10366275051831375No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTGGG108.4175915E-4295.04
CTGGGTT206.0701495E-8295.09
GTACTGG206.0701495E-8295.05
TCGGTTG108.4175915E-4295.02
TCGGTAC206.0701495E-8295.02
GGTTACG108.4175915E-4295.04
TTGGGAT108.4175915E-4295.06
CTGGATT255.16593E-10295.09
CGGGATT108.4175915E-4295.09
ACGGGAT108.4175915E-4295.08
GTTGGGA108.4175915E-4295.05
CGGTTGG108.4175915E-4295.03
CGGTTAC25600.0295.03
ACTGGGT206.0701495E-8295.08
ACTGGAT255.16593E-10295.08
GGTACTG206.0701495E-8295.04
GTTACGG108.4175915E-4295.05
TTACGGG108.4175915E-4295.06
ATCGGTT25800.0294.428281
TCGGTTA25650.0294.424962
Produced by FastQC (version 0.11.5)