FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-115-4-1-3-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-115-4-1-3-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25546
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC450717.64268378611133No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA23439.17169028419322No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATTCCTCTG20888.173490957488452No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATTCCT13855.421592421514132No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA12074.724810146402568No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT10454.090659985907774No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATTCCT5912.3134737336569327No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATTCCTCT5712.2351835903859705No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATTCC4871.906364988647929No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATTCCTC4841.894621467157285No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT4651.8202458310498708No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC4331.6949816018163315No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATTCC3971.5540593439285992No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT3691.4444531433492522No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA3101.213497220699914No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT2651.0373443983402488No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATTCC2310.9042511547796133No Hit
ATCGGTTACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAT2090.8181319971815547No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA2020.7907304470367181No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT2000.7829014327096219No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT1990.7789869255460737No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT1960.7672434040554293No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC1860.7280983324199484No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA1810.7085257966022077No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATT1780.6967822751115634No Hit
ATCGGTTACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGT1720.6732952321302748No Hit
ATCGGTTACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATTCCT1680.6576372034760825No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAT1630.6380646676583418No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTATTCC1320.5167149455883504No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAT1300.5088859312612543No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT1290.504971424097706No Hit
ATCGGTTACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTATTCCT1080.42276677366319576No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC1080.42276677366319576No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTTCCTCTGTCTCTTATA960.3757926877006185No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAT880.34447663039223364No Hit
ATCGGTTACTGGGATTAGATACCCCACGTCTAGAGGAGCCTGTTCTATTC870.3405621232286855No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCT810.3170750802473969No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT810.3170750802473969No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC800.31316057308384876No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT780.3053315587567525No Hit
ATCGGTTACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTT760.2975025444296563No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT750.29358803726610816No Hit
ATCGGTTACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTATTC740.2896735301025601No Hit
ATCGGTTACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT730.285759022939012No Hit
ATCGGTTACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTATTCCT690.27010099428481954No Hit
ATCGGTTACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTATTC650.2544429656306271No Hit
ATCGGTTACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTATTC650.2544429656306271No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTCTCATACCATCTGAGCTAGAG620.24269944413998276No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTATT590.23095592264933845No Hit
ATCGGTTACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAT590.23095592264933845No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT580.22704141548579035No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT540.21138338683159788No Hit
ATCGGTTACTGGGATTAGATACCCCGACAACCTAGAGGAGCCTGTTCTAT530.2074688796680498No Hit
ATCGGTTACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTAT520.2035543725045017No Hit
ATCGGTTACTGGGATTAGATACCCCACACTAGAGGAGCCTGTTCTATTCC520.2035543725045017No Hit
ATCGGTTACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTA520.2035543725045017No Hit
ATCGGTTACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC520.2035543725045017No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGTCTAGAGGAGCCTGTTCTAT500.19572535817740547No Hit
ATCGGTTACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATTCCTC490.19181085101385734No Hit
ATCGGTTACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTATTCC480.18789634385030926No Hit
ATCGGTTACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT440.17223831519611682No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT440.17223831519611682No Hit
ATCGGTTACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTATTC430.1683238080325687No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT420.1644093008690206No Hit
ATCGGTTACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT410.16049479370547248No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTA410.16049479370547248No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT400.15658028654192438No Hit
ATCGGTTACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTATTC380.14875127221482815No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG350.13700775072418384No Hit
ATCGGTTACTGGGATTAGATACCCCTGTCAGACTAGAGGAGCCTGTTCTA340.1330932435606357No Hit
ATCGGTTACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTATTC320.1252642292335395No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATA300.11743521490644326No Hit
ATCGGTTACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA300.11743521490644326No Hit
ATCGGTTACTGGGATTAGATACCCCGCACGATTTCATTGACTAGAGGAGC280.10960620057934706No Hit
ATCGGTTACTGGGATTAGATACCCCGAGATCGAAAGAGCGCTAGAGGAGC270.10569169341579894No Hit
ATCGGTTACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTATTC270.10569169341579894No Hit
ATCGGTTACTGGGATTAGATACCCCGACCCTAGAGGAGCCTGTTCTATTC260.10177718625225085No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTGG25250.0294.9516
GGTTACG108.420831E-4294.9514
ATCGGTT25450.0294.9511
CGGTTAT108.420831E-4294.9513
GTTACGG108.420831E-4294.9515
TCGGTTA25500.0294.372622
CGGTTAC25400.0294.370363
GGTTACT25300.0294.368074
TACTGGG25300.0294.368077
GTTACTG25300.0294.368075
CTGGGAT25250.0294.36699
ACTGGGA25250.0294.36698
GGTTATG150.0028348581196.633994
TATGTAA559.365971E-6107.25489295
CCGCTCG351.4006218E-859.10609465-69
CGCTCGC351.4006218E-859.10609465-69
ACCGCTC351.4006218E-859.10609465-69
CTGGCCC650.059.10609430-34
ATGCCTG650.059.10609425-29
GCCGTAA1700.059.10609435-39
Produced by FastQC (version 0.11.5)