FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-114-5-1-3-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-114-5-1-3-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24894
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC733929.48099943761549No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA20548.250984172893066No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT18417.395356310757613No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAAGTCTCCT14045.639913232104121No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT7783.125251064513537No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAAGTCT7543.0288422913151765No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT7392.9685868080662003No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA6162.4744918454246No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT4231.6992046276211135No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC3891.5626255322567686No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAAGTCTCC3431.3778420502932434No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT3261.309552502611071No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAAGTCTC2631.0564794729653733No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAAGTCT2290.9199003776010283No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC2100.8435767654856591No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT2050.8234916044026673No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT2000.8034064433196755No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA1980.7953723788864787No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAAGTC1970.7913553466698803No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC1880.7552020567204949No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAAGTC1760.7069976701213143No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1680.6748614123885274No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT1580.6346910902225436No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC1380.554350445890576No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAA1220.49007793042500203No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT1190.4780268337752068No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT1130.45392464047561665No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT1000.40170322165983774No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAA960.3856350927934442No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT950.38161806057684583No Hit
ATCGGTTACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAAGTCT880.35349883506065716No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT880.35349883506065716No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT860.34546477062746045No Hit
ATCGGTTACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAAG760.30529444846147663No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG720.28922631959508316No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAA720.28922631959508316No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT690.277175222945288No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT660.2651241262954929No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT620.24905599742909937No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAA570.22897083634610751No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAAGTC530.212902707479714No Hit
ATCGGTTACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAAGTCT510.20486864304651722No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA490.1968345786133205No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAAGTC480.1928175463967221No Hit
ATCGGTTACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAAG470.18880051418012372No Hit
ATCGGTTACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA460.18478348196352537No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAAG450.18076644974692696No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTA450.18076644974692696No Hit
ATCGGTTACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTA440.17674941753032858No Hit
ATCGGTTACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAAGTCT430.17273238531373022No Hit
ATCGGTTACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAAGT420.16871535309713184No Hit
ATCGGTTACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAAGTCT420.16871535309713184No Hit
ATCGGTTACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAAGTCT420.16871535309713184No Hit
ATCGGTTACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAAGTCTC410.16469832088053346No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCT390.15666425644733672No Hit
ATCGGTTACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAAGT380.15264722423073832No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG340.13657909536434482No Hit
ATCGGTTACTGGGATTAGATACCCCTTCTAGAGGAGCCTGTTCTAAGTCT330.13256206314774646No Hit
ATCGGTTACTGGGATTAGATACCCCCGGACCTAGAGGAGCCTGTTCTAAG330.13256206314774646No Hit
ATCGGTTACTGGGATTAGATACCCCATGTGCTAGAGGAGCCTGTTCTAAG310.12452799871454968No Hit
ATCGGTTACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA310.12452799871454968No Hit
ATCGGTTACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA310.12452799871454968No Hit
ATCGGTTACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTAAGTC300.1205109664979513No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAAGTC300.1205109664979513No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAA290.11649393428135293No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCTGCTAGAGGAGCCTGTTCT290.11649393428135293No Hit
ATCGGTTACTGGGATTAGATACCCCTGACCTGCTAGAGGAGCCTGTTCTA280.11247690206475455No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGAT280.11247690206475455No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAAT270.10845986984815618No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAAT270.10845986984815618No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC270.10845986984815618No Hit
ATCGGTTACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAAGT260.10444283763155782No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGAT250.10042580541495943No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTAG108.4153493E-4295.06
TATGTTT108.4153493E-4295.0295
TCGGTTA24700.0295.02
ATCGGTT24700.0295.01
TACTGGA157.1556005E-6295.07
ACTGGAT157.1556005E-6295.08
CGGTTAC24700.0294.402833
ACTGGGA24400.0294.39558
CTGGGAT24350.0294.394269
TACTGGG24350.0294.394267
GGTTACT24700.0293.805664
GTTACTG24600.0293.80085
TTACTGG24550.0293.798376
CTGGATT202.2554499E-5221.259
TACTAGG150.002833015196.666677
CTAGGAT200.0066983565147.59
CTTTTAT150.0043713359.000004160-164
AGCCCAA150.0043713359.000004175-179
AAACCTT1800.059.00000440-44
TTCATAT7650.059.000004120-124
Produced by FastQC (version 0.11.5)