FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-113-4-7-5-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-113-4-7-5-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24389
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG457718.766657099512074No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGG281211.52978801918898No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGGAATCGCTG23949.815900610931157No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGGAATCG13975.727992127598507No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG9253.7926934273647954No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAGG7092.9070482594612326No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAGG5582.2879166837508715No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGGAATCG4861.9927016277830172No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGGAATCGCT4791.9640001640083642No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGGAATC4741.9434991184550412No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC4691.922998072901718No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG3571.463774652507278No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGGAATC3401.394071097625979No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT3371.381770470293985No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT2991.2259625240887286No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGGAATCGC2721.1152568781007832No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAG2631.0783549961048013No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGGAA2270.9307474681208742No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG2100.8610439132395752No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAG1760.7216368034769773No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAGG1720.7052359670343188No Hit
AAGCGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG1510.6191315757103613No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGGAATC1410.5781294846037147No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAG1400.57402927549305No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAG1350.553528229939727No Hit
AAGCGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGGAAT1280.5248267661650744No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG1200.4920250932797573No Hit
AAGCGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT1200.4920250932797573No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGGA1160.47562425683709864No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG1030.4223215383984584No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGGAATCG1020.4182213292877937No Hit
AAGCGACTGGGATTAGATACCCCTGACGCATAGAGGAGCCTGTTCTAGGA1000.4100209110664644No Hit
AAGCGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGGAATCG960.3936200746238058No Hit
AAGCGACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCTGT910.37311902907048256No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG860.3526179835171594No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG850.34851777440649473No Hit
AAGCGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGGAAT780.31981631063184224No Hit
AAGCGACTGGGATTAGATACCCCCACGAACCTAGAGGAGCCTGTTCTAGG750.3075156832998483No Hit
AAGCGACTGGGATTAGATACCCCTGTCAGACTAGAGGAGCCTGTTCTAGG710.2911148468571897No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG710.2911148468571897No Hit
AAGCGACTGGGATTAGATACCCCCACGACCTAGAGGAGCCTGTTCTAGGA700.287014637746525No Hit
AAGCGACTGGGATTAGATACCCCACCCCTAGAGGAGCCTGTTCTAGGAAT640.2624133830825372No Hit
AAGCGACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGGAATC620.2542129648612079No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAGGA620.2542129648612079No Hit
AAGCGACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCTG610.25011275575054326No Hit
AAGCGACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAGGA590.24191233752921398No Hit
AAGCGACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGAGC580.23781212841854932No Hit
AAGCGACTGGGATTAGATACCCCGAACCCACGAGACTAGAGGAGCCTGTT560.22961171019722004No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT550.2255115010865554No Hit
AAGCGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAGGA540.22141129197589077No Hit
AAGCGACTGGGATTAGATACCCCAATGTCTAGAGGAGCCTGTTCTAGGAA530.21731108286522613No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG520.2132108737545615No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG490.20091024642256758No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG490.20091024642256758No Hit
AAGCGACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAGGAATC490.20091024642256758No Hit
AAGCGACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAGGAA480.1968100373119029No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAGGA470.19270982820123825No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG430.1763089917585797No Hit
AAGCGACTGGGATTAGATACCCCACGAACCTAGAGGAGCCTGTTCTAGGA420.17220878264791506No Hit
AAGCGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAGGAAT420.17220878264791506No Hit
AAGCGACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTAGGA410.1681085735372504No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG410.1681085735372504No Hit
AAGCGACTGGGATTAGATACCCCGCGAGACCTAGAGGAGCCTGTTCTAGG400.16400836442658576No Hit
AAGCGACTGGGATTAGATACCCCAAACCTAGAGGAGCCTGTTCTAGGAAT380.15580794620525645No Hit
AAGCGACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTAGGAAT380.15580794620525645No Hit
AAGCGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGGAATCG370.15170773709459182No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT370.15170773709459182No Hit
AAGCGACTGGGATTAGATACCCCAGTGCAGCTGTTGGGGACTAGAGGAGC350.1435073188732625No Hit
AAGCGACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTAGGAATCGC330.13530690065193327No Hit
AAGCGACTGGGATTAGATACCCCGCCACCTAGAGGAGCCTGTTCTAGGAA330.13530690065193327No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCTA320.1312066915412686No Hit
AAGCGACTGGGATTAGATACCCCGACGAGAGGAGCCTGTTCTAGGAATCG310.12710648243060396No Hit
AAGCGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGGAATCG290.11890606420927466No Hit
AAGCGACTGGGATTAGATACCCCCATAATATTAGCTAGAGGAGCCTGTTC280.11480585509861002No Hit
AAGCGACTGGGATTAGATACCCCGCCACGAGACCTAGAGGAGCCTGTTCT270.11070564598794538No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG260.10660543687728075No Hit
AAGCGACTGGGATTAGATACCCCGAGATCGAAAGAGCGCTAGAGGAGCCT250.1025052277666161No Hit
AAGCGACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGGAAT250.1025052277666161No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATTG108.414308E-4295.09
ACTGGGA24150.0295.06
GACTGGG24250.0294.999975
CGACTGG24250.0294.999974
CTGGGAT24200.0294.39057
GCGACTG24350.0293.78853
GGGATTA24150.0293.167729
AGCGACT24450.0292.58692
TGGGATT24350.0292.5778
AAGCGAC24700.0289.625521
ACTGGGT150.0028326656196.666676
GCCCTAA6250.059.00000430-34
CGCTTAA501.8189894E-1259.00000490-94
CACTGCT257.792418E-659.00000420-24
AACTTTG501.8189894E-1259.00000440-44
ACTTTGA501.8189894E-1259.00000440-44
CCACTGC257.792418E-659.00000420-24
CAGGGAA1250.059.00000470-74
GCTCGCC4950.059.00000465-69
ACCCCGG501.8189894E-1259.00000415-19
Produced by FastQC (version 0.11.5)