FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-112-4-1-3-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-112-4-1-3-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24663
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC394816.007784941004743No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGACGTGGC256510.400194623525119No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA24399.889307870088796No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA9143.705956290799984No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGACGT8473.4342942869886066No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT8323.3734744353890442No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGACG5502.230061225317277No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA5232.120585492438065No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGACGTGG4871.974617848599116No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGACGT4101.6624092770546974No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGACG4001.6218627093216558No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGACGTG3941.597534768681831No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT3871.569152171268702No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT3871.569152171268702No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC3501.4191298706564488No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT3471.4069659003365365No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGACG3361.362364675830191No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT3281.3299274216437578No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT3181.2893808539107166No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA2911.1799051210315046No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGA2771.1231399262052468No Hit
ATCGGTTACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTAG2711.098811985565422No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT1980.8028220411142197No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAG1780.7217289056481369No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC1660.6730730243684873No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAG1520.6163078295422292No Hit
ATCGGTTACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT1500.6081985159956209No Hit
ATCGGTTACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGACGT1330.5392693508494506No Hit
ATCGGTTACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGACGT1210.4906134695698009No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT1190.48250415602319263No Hit
ATCGGTTACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTA1180.47844949924988844No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT1150.4662855289299761No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC1040.42168430442363053No Hit
ATCGGTTACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCC1000.40546567733041394No Hit
ATCGGTTACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTAGACGTG990.40141102055710987No Hit
ATCGGTTACTGGGATTAGATACCCCAACGAGACCTAGAGGAGCCTGTTCT970.3933017070105016No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAG950.3851923934638933No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT920.3730284231439809No Hit
ATCGGTTACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGAC860.34870048250415603No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT840.34059116895754776No Hit
ATCGGTTACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGACGT840.34059116895754776No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAGACG740.3000446012245063No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT720.29193528767789806No Hit
ATCGGTTACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAGAC720.29193528767789806No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAG720.29193528767789806No Hit
ATCGGTTACTGGGATTAGATACCCCAATCTAGAGGAGCCTGTTCTAGACG710.2878806309045939No Hit
ATCGGTTACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGACGTG690.27977131735798566No Hit
ATCGGTTACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAGAC610.24733406317155254No Hit
ATCGGTTACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT590.23922474962494422No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT590.23922474962494422No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA570.23111543607833596No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC560.22706077930503182No Hit
ATCGGTTACTGGGATTAGATACCCCCCGCTAGAGGAGCCTGTTCTAGACG520.21084215221181526No Hit
ATCGGTTACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT520.21084215221181526No Hit
ATCGGTTACTGGGATTAGATACCCCCCAACCTAGAGGAGCCTGTTCTAGA520.21084215221181526No Hit
ATCGGTTACTGGGATTAGATACCCCCCACGAGACCTAGAGGAGCCTGTTC490.19867818189190284No Hit
ATCGGTTACTGGGATTAGATACCCCCGTGGACTAGAGGAGCCTGTTCTAG480.1946235251185987No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAG480.1946235251185987No Hit
ATCGGTTACTGGGATTAGATACCCCCGAGGACCTAGAGGAGCCTGTTCTA480.1946235251185987No Hit
ATCGGTTACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGAC480.1946235251185987No Hit
ATCGGTTACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTAGA470.19056886834529457No Hit
ATCGGTTACTGGGATTAGATACCCCATCAGACTAGAGGAGCCTGTTCTAG450.1824595547986863No Hit
ATCGGTTACTGGGATTAGATACCCCGATGTCTAGAGGAGCCTGTTCTAGA440.17840489802538215No Hit
ATCGGTTACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGACGT440.17840489802538215No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGACG430.17435024125207801No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT410.16624092770546972No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT390.15813161415886146No Hit
ATCGGTTACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGAC380.1540769573855573No Hit
ATCGGTTACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA370.15002230061225316No Hit
ATCGGTTACTGGGATTAGATACCCCAAAGACCTAGAGGAGCCTGTTCTAG360.14596764383894903No Hit
ATCGGTTACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAGA340.13785833029234076No Hit
ATCGGTTACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTC300.1216397031991242No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATA300.1216397031991242No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT290.11758504642582004No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCTGCTAGAGGAGCCTGTTCT290.11758504642582004No Hit
ATCGGTTACTGGGATTAGATACCCCGGAACTAGAGGAGCCTGTTCTAGAC280.11353038965251591No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG270.10947573287921177No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT270.10947573287921177No Hit
ATCGGTTACTGGGATTAGATACCCCCGCCTAGAGGAGCCTGTTCTAGACG260.10542107610590763No Hit
ATCGGTTACTGGGATTAGATACCCCTCTCCGAGCCCACGAGACTCTCCGA260.10542107610590763No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGTT24550.0294.39921
TCGGTTA24450.0294.396732
GGTTACT24250.0294.391754
TTACTGG24150.0294.389256
TACTGGG24000.0294.38547
ACTGGGA24000.0294.38548
CGGTTAC24450.0293.793463
GTTACTG24250.0293.78355
CTGGGAT24100.0293.77599
TCGGTTC150.0028328772196.666662
CTGGATT150.0028328772196.666669
TACTGGA255.4911485E-5177.07
ACTGGAT255.4911485E-5177.08
AATCTTG452.5465852E-1159.00000440-44
AAATCTT452.5465852E-1159.00000440-44
CTTTACC452.5465852E-1159.00000455-59
TTACTTT452.5465852E-1159.00000450-54
GATACTT452.5465852E-1159.00000445-49
GCCTAGC1250.059.00000430-34
GTATCCG452.5465852E-1159.00000465-69
Produced by FastQC (version 0.11.5)