FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-111-5-7-5-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-111-5-7-5-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23662
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG755631.933057222550925No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGA17977.594455244696137No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG15886.711182486687515No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAGACT12985.485588707632491No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC6852.8949370298368695No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG6042.5526160087904657No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGATTGAG5632.3793424055447554No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT4231.7876764432423295No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAGA3481.47071253486603No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG3081.3016651170653368No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAGA2591.0945820302594877No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGATTGAGAC2511.0607725466993492No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG2190.9255346124587946No Hit
AAGCGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGATTGAG2110.8917251288986562No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGATTGA1960.8283323472233962No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAG1840.7776181218831882No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG1750.7395824528780323No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGATTGA1720.7269038965429803No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT1710.722677711097963No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAG1650.697320598427859No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGATTGAGA1620.684642042092807No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGATTGAG1530.646606373087651No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG1360.5747612205223566No Hit
AAGCGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAGAT1310.5536302932972699No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGATT1260.5324993660721833No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGATTGA1240.5240469951821486No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG1240.5240469951821486No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG1180.4986898825120446No Hit
AAGCGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGATTGA1120.4733327698419406No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGAT1010.4268447299467501No Hit
AAGCGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAGAT1000.4226185445017327No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG990.4183923590567154No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT960.40571380272166346No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAGA940.39726143183162876No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG910.38458287549657677No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG860.36345194827149013No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAG860.36345194827149013No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC850.35922576282647284No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG850.35922576282647284No Hit
AAGCGACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAGA820.34654720649142084No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAG710.3000591665962303No Hit
AAGCGACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAGA680.2873806102611783No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG660.2789282393711436No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAGAT650.27470205392612623No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT610.257797312146057No Hit
AAGCGACTGGGATTAGATACCCCACAAGGCCTAGAGGAGCCTGTTCTAGA560.2366663849209703No Hit
AAGCGACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTTC520.21976164314090105No Hit
AAGCGACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAGA520.21976164314090105No Hit
AAGCGACTGGGATTAGATACCCCATGTGTATAGAGGAGCCTGTTCTAGAT510.2155354576958837No Hit
AAGCGACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGATTG490.20708308680584905No Hit
AAGCGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGATTGAG490.20708308680584905No Hit
AAGCGACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAGA490.20708308680584905No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTGTT470.19863071591581438No Hit
AAGCGACTGGGATTAGATACCCCATAATCCTAGAGGAGCCTGTTCTAGAT440.1859521595807624No Hit
AAGCGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT400.1690474178006931No Hit
AAGCGACTGGGATTAGATACCCCCACGAACCTAGAGGAGCCTGTTCTAGA380.16059504691065843No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT380.16059504691065843No Hit
AAGCGACTGGGATTAGATACCCCGAGATCGAAAGAGCGCTAGAGGAGCCT370.1563688614656411No Hit
AAGCGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGATTG370.1563688614656411No Hit
AAGCGACTGGGATTAGATACCCCACGACCTAGAGGAGCCTGTTCTAGATT360.15214267602062378No Hit
AAGCGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGAT360.15214267602062378No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG350.14791649057560646No Hit
AAGCGACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTTC350.14791649057560646No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT340.14369030513058914No Hit
AAGCGACTGGGATTAGATACCCCCGAGCCACGAGACCTAGAGGAGCCTGT330.1394641196855718No Hit
AAGCGACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAGA330.1394641196855718No Hit
AAGCGACTGGGATTAGATACCCCGATGTCCAGAGGAGCCTGTTCTAGATT330.1394641196855718No Hit
AAGCGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG310.13101174879553715No Hit
AAGCGACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGATTGAGA310.13101174879553715No Hit
AAGCGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAGATTG300.12678556335051983No Hit
AAGCGACTGGGATTAGATACCCCACGAGAGCTAGAGGAGCCTGTTCTAGA290.1225593779055025No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG280.11833319246048515No Hit
AAGCGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGATTGAG280.11833319246048515No Hit
AAGCGACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAGATTG270.11410700701546785No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATTA260.10988082157045052No Hit
AAGCGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT250.10565463612543317No Hit
AAGCGACTGGGATTAGATACCCCAAACCTAGAGGAGCCTGTTCTAGATTG250.10565463612543317No Hit
AAGCGACTGGGATTAGATACCCCTGATGCCGACTAGAGGAGCCTGTTCTA240.10142845068041587No Hit
AAGCGACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCTA240.10142845068041587No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACAG240.10142845068041587No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGGA108.41404E-4294.98525
ATGGGAT108.41404E-4294.98526
GCGATGG108.41404E-4294.98523
CTGGATT350.0294.98527
GACTGGG22900.0294.98525
GACTGGA350.0294.98525
ACTGGGA22850.0294.98526
CGACTGG23250.0294.98524
GCGACTG23300.0294.352173
CTGGGAT22950.0294.342537
TGGGATT23050.0293.065558
AAGCGAC23600.0292.485321
AGCGACT23550.0292.480042
GGGATTA23150.0292.436749
TGGATTA400.0258.112068
ACTGGAT400.0258.112066
CGATGGG150.0028325762196.65684
TAAACGA352.0975168E-4126.422226295
GCCGTAA3050.059.1220430-34
AGCCGTA3050.059.1220430-34
Produced by FastQC (version 0.11.5)