FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-110-5-7-5-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-110-5-7-5-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23669
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG807634.120579661160164No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG18307.731632092610588No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAAT17177.254214373230808No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAATAGGATTCT9634.068612953652457No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG7623.219400904136212No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC7062.9828045122311884No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAATAGGAT4962.09556804258735No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT4001.6899742278930245No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG3481.4702775782669315No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAATAGGA3361.4195783514301408No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAATAGGATTC3221.3604292534538849No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT2831.195656766234315No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAAT2631.1111580548396636No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAATAGGA2491.052008956863408No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG2130.8999112763530356No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAAT1990.8407621783767798No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAATAGGAT1910.8069626938189193No Hit
AAGCGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAATA1890.7985128226794541No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT1740.7351387891334658No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG1720.7266889179940006No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG1540.6506400777388145No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG1350.5703663019138958No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG1200.5069922683679073No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAATAGGATT1170.4943174616587097No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAA1090.46051797710084924No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAA1070.45206810596138414No Hit
AAGCGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT1070.45206810596138414No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG1030.4351683636824539No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG1000.42249355697325613No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAATA960.4055938146943259No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAA930.39291900798512824No Hit
AAGCGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAATAGGAT930.39291900798512824No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG840.3548945878575352No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG790.33376991000887235No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAATA720.30419536102074446No Hit
AAGCGACTGGGATTAGATACCCCATAATCCTAGAGGAGCCTGTTCTAATA690.2915205543115468No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC690.2915205543115468No Hit
AAGCGACTGGGATTAGATACCCCTGACGCTAGAGGAGCCTGTTCTAATAG660.2788457476023491No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG650.27462081203261646No Hit
AAGCGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAATAGG560.23659639190502343No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAATAG510.21547171405636062No Hit
AAGCGACTGGGATTAGATACCCCCGAGCCCACGAGACCTAGAGGAGCCTG480.20279690734716296No Hit
AAGCGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAATAGGAT480.20279690734716296No Hit
AAGCGACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTAATAG450.19012210063796528No Hit
AAGCGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAATA450.19012210063796528No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAAT450.19012210063796528No Hit
AAGCGACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAATAGGAT440.1858971650682327No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAATAGGAT420.1774472939287676No Hit
AAGCGACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAA410.17322235835903502No Hit
AAGCGACTGGGATTAGATACCCCCGAGCCTAGAGGAGCCTGTTCTAATAG400.16899742278930246No Hit
AAGCGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA390.1647724872195699No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT360.15209768051037223No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG350.14787274494063968No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT340.1436478093709071No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAA340.1436478093709071No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT330.13942287380117455No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG320.13519793823144197No Hit
AAGCGACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCTGT310.1309730026617094No Hit
AAGCGACTGGGATTAGATACCCCACGGACCTAGAGGAGCCTGTTCTAATA310.1309730026617094No Hit
AAGCGACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCTG310.1309730026617094No Hit
AAGCGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAATAGGAT310.1309730026617094No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAACAG310.1309730026617094No Hit
AAGCGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG310.1309730026617094No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG300.12674806709197683No Hit
AAGCGACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAATAGGATT300.12674806709197683No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAATAGGA300.12674806709197683No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTAA300.12674806709197683No Hit
AAGCGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG290.12252313152224428No Hit
AAGCGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT280.11829819595251172No Hit
AAGCGACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAAT280.11829819595251172No Hit
AAGCGACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGCCT280.11829819595251172No Hit
AAGCGACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTTC280.11829819595251172No Hit
AAGCGACTGGGATTAGATACCCCCGAGCTAGAGGAGCCTGTTCTAATAGG270.11407326038277915No Hit
AAGCGACTGGGATTAGATACCCCGCCCGAGACCTAGAGGAGCCTGTTCTA270.11407326038277915No Hit
AAGCGACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA260.1098483248130466No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAATA260.1098483248130466No Hit
AAGCGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAATAGGA250.10562338924331403No Hit
AAGCGACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAAT240.10139845367358148No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTG23550.0295.03
CTGGGAT23300.0295.07
GGGATTA23250.0295.09
GACTGGG23350.0295.05
TGGGATT23250.0295.08
ACTGGGA23300.0295.06
AGCGACT23600.0295.02
CTGGATT108.412777E-4294.999977
CGACTGG23500.0294.372344
AAGCGAC23700.0293.755281
GCAGTAA200.0066963197147.49998295
TGGATTA200.0066963197147.499988
GACTGGA200.0066963197147.499985
ACTGGAT200.0066963197147.499986
ATCCTAG150.004370808659.00000425-29
AAACCCT257.791035E-659.00000435-39
CAGTTAA10050.059.00000445-49
CCCGGTA150.004370808659.00000420-24
CCTGTAG501.8189894E-1259.00000420-24
GCCTAGC2000.059.00000425-29
Produced by FastQC (version 0.11.5)