FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-11-2-5-7-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-11-2-5-7-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11114
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG7787.0001799532121645No Hit
CTGGATGGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG5605.03868994061544No Hit
CTGGATGGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG5404.858736728450603No Hit
CTGGATGGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCG3613.2481554795753107No Hit
CTGGATGGTGTCAGCCGCCGCGGTAATACGGAGGGGGTTAGCGTTGTTCG3423.0771999280187154No Hit
CTGGATGGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG2532.27640813388519No Hit
CTGGATGGTGCCAGCAGCCGCGGTAATACGGAGGGGGTTAGCGTTGTTCG2392.150440885369804No Hit
CTGGATGGTGTCAGCAGCCGCGGTAATACGGAGGGGGTTAGCGTTGTTCG2272.0424689580709017No Hit
CTGGATGGTGTCAGCCGCCGCGGTAATACATAGGGTGCAAGCGTTGTCCG1731.5565952852258413No Hit
CTGGATGGTGTCAGCCGCCGCGTAGTCCTTCCTCTGTCTCTTATACACAT1641.4756163397516646No Hit
CTGGATGGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1641.4756163397516646No Hit
CTGGATGGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1611.448623357926939No Hit
CTGGATGGTGTCAGCAGCCGCGTAGTCCTTCCTCTGTCTCTTATACACAT1571.4126327154939715No Hit
CTGGATGGTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCG1501.3496490912362786No Hit
CTGGATGGTGCCAGCAGCCGCGGTAATACATAGGGTGCAAGCGTTGTCCG1451.3046607881950694No Hit
CTGGATGGTGCCAGCAGCCGCGTAGTCCTTCCTCTGTCTCTTATACACAT1411.268670145762102No Hit
CTGGATGGTGCCAGCCGCCGCGGTAATACGGAGGGGGTTAGCGTTGTTCG1381.2416771639373763No Hit
CTGGATGGTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG1371.2326795033291345No Hit
CTGGATGGTGTCAGCCGCCGCGGTAATACGGAGGATCCAAGCGTTATCCG1311.1786935396796834No Hit
CTGGATGGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCG1251.124707576030232No Hit
CTGGATGGTGTCAGCAGCCGCGGTAATACATAGGGTGCAAGCGTTGTCCG1231.1067122548137485No Hit
CTGGATGGTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCG1211.0887169335972648No Hit
CTGGATGGTGTCAGCCGCCGCGGTAAGACGGAGGATGCAAGTGTTATCCG1100.9897426669066043No Hit
CTGGATGGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCG1050.944754363865395No Hit
CTGGATGGTGCCAGCCGCCGCGGTAATACATAGGGTGCAAGCGTTGTCCG990.8907684002159438No Hit
CTGGATGGTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG960.8637754183912183No Hit
CTGGATGGTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCG910.818787115350009No Hit
CTGGATGGTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCG880.7917941335252834No Hit
CTGGATGGTGCCAGCAGCCGCGGTAATACGGAGGATCCAAGCGTTATCCG860.7737988123087997No Hit
CTGGATGGTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG840.7558034910923159No Hit
CTGGATGGTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG810.7288105092675904No Hit
CTGGATGGTGTCAGCAGCCCGGTAGTCCTTCCTCTGTCTCTTATACACAT810.7288105092675904No Hit
CTGGATGGTGTCAGCCGCCGCGGTAATACGGAGGGTCCGAGCGTTAATCG800.7198128486593486No Hit
CTGGATGGTGCCAGCCGCCGCGTAGTCCTTCCTCTGTCTCTTATACACAT790.7108151880511067No Hit
CTGGATGGTGTCAGCAGCCGCGGTAGTCCTTCCTCTGTCTCTTATACACA770.692819866834623No Hit
CTGGATGGTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG750.6748245456181393No Hit
CTGGATGGTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCG750.6748245456181393No Hit
CTGGATGGTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCG750.6748245456181393No Hit
CTGGATGGTGTCAGCCGCCGGGTAGTCCTTCCTCTGTCTCTTATACACAT730.6568292244016556No Hit
CTGGATGGTGCCAGCAGCCGCGGTAAGACGGAGGATGCAAGTGTTATCCG720.6478315637934137No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT710.6388339031851719No Hit
CTGGATGGTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCG700.62983624257693No Hit
CTGGATGGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTACTCG700.62983624257693No Hit
CTGGATGGTGTCAGCCGCCGAGTAGTCCTTCCTCTGTCTCTTATACACAT690.6208385819686881No Hit
CTGGATGGTGTCAGCCGCCGCGGTAGTCCTTCCTCTGTCTCTTATACACA670.6028432607522044No Hit
CTGGATGGTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG650.5848479395357207No Hit
CTGGATGGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTACTCG620.5578549577109951No Hit
CTGGATGGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTACTCG610.5488572971027532No Hit
CTGGATGGTGCCAGCAGCCGCGGTAATACGGAGGGTCCGAGCGTTAATCG600.5398596364945114No Hit
CTGGATGGTGTCAGCCGCCCGGTAGTCCTTCCTCTGTCTCTTATACACAT590.5308619758862696No Hit
CTGGATGGTGTCAGCAGCCGCGGTAATACGGAGGATCCAAGCGTTATCCG590.5308619758862696No Hit
CTGGATGGTGTCAGCAGCCGCGGTAAGACGGAGGATGCAAGTGTTATCCG570.5128666546697859No Hit
CTGGATGGTGCCAGCAGCCGCGGTAGTCCTTCCTCTGTCTCTTATACACA570.5128666546697859No Hit
CTGGATGGTGTCAGCAGCCGAGTAGTCCTTCCTCTGTCTCTTATACACAT520.46787835162857655No Hit
CTGGATGGTGTCAGCAGCCGCGGTAATACGGAGGGTCCGAGCGTTAATCG520.46787835162857655No Hit
CTGGATGGTGTCAGCAGCCGTGTAGTCCTTCCTCTGTCTCTTATACACAT520.46787835162857655No Hit
CTGGATGGTGCCAGCCGCCGCGGTAAGACGGAGGATGCAAGTGTTATCCG510.45888069102033474No Hit
CTGGATGGTGCCAGCAGCCGAGTAGTCCTTCCTCTGTCTCTTATACACAT500.4498830304120929No Hit
CTGGATGGTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCG500.4498830304120929No Hit
CTGGATGGTGTCAGCCGCCGTGTAGTCCTTCCTCTGTCTCTTATACACAT500.4498830304120929No Hit
CTGGATGGTGCCAGCCGCCGCGGTAATACGGAGGGTCCGAGCGTTAATCG490.44088536980385096No Hit
CTGGATGGTGCCAGCAGCCCGGTAGTCCTTCCTCTGTCTCTTATACACAT480.43188770919560915No Hit
CTGGATGGTGCCAGCAGCCGCGGTAATACGGAGGGTCCGAGCGTTATCCG450.40489472737088356No Hit
CTGGATGGTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCG440.3958970667626417No Hit
CTGGATGGTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTGTCCG420.37790174554615796No Hit
CTGGATGGTGTCAGCCGCCGCGGTAATACGGAGGGTCCGAGCGTTATCCG410.36890408493791615No Hit
CTGGATGGTGCCAGCCGCCGCGGTAGTCCTTCCTCTGTCTCTTATACACA410.36890408493791615No Hit
CTGGATGGTGTCAGCAGCCGGGTAGTCCTTCCTCTGTCTCTTATACACAT390.3509087637214324No Hit
CTGGATGGTGCCAGCCGCCCGGTAGTCCTTCCTCTGTCTCTTATACACAT380.34191110311319056No Hit
CTGGATGGTGCCAGCCGCCGCGGTAATACGGAGGATCCAAGCGTTATCCG380.34191110311319056No Hit
CTGGATGGTGCCAGCAGCCGGGTAGTCCTTCCTCTGTCTCTTATACACAT380.34191110311319056No Hit
CTGGATGGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTACTCG370.3329134425049487No Hit
CTGGATGGTGTCAGCAGCCGCGGTAATACGGAGGGTCCGAGCGTTATCCG350.314918121288465No Hit
CTGGATGGTGCCAGCCGCCGGGTAGTCCTTCCTCTGTCTCTTATACACAT330.29692280007198124No Hit
CTGGATGGTGCCAGCAGCCGTGTAGTCCTTCCTCTGTCTCTTATACACAT310.27892747885549757No Hit
CTGGATGGTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTGTCCG280.25193449703077203No Hit
CTGGATGGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTGTCCG260.23393917581428827No Hit
CTGGATGGTGCCAGCCGCCGAGTAGTCCTTCCTCTGTCTCTTATACACAT240.21594385459780457No Hit
CTGGATGGTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTGTCCG240.21594385459780457No Hit
CTGGATGGTGCCAGCAGCCGCGGTAATACGAGGGGTGCAAGCGTTAATCG230.20694619398956274No Hit
CTGGATGGTGCCAGCCGCCGTGTAGTCCTTCCTCTGTCTCTTATACACAT230.20694619398956274No Hit
CTGGATGGTGCCAGCCGCCGCGGTAATACGGAGGGTCCGAGCGTTATCCG220.19794853338132085No Hit
CTGGATGGTGTCAGCCGCCGCGGTAATACGGAGGATGCAAGCGTTATCCG210.18895087277307898No Hit
CTGGATGGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTGTCCG200.17995321216483715No Hit
CTGGATGGTGTCAGCCGCCGCGGTAATACGAGGGGTGCAAGCGTTAATCG200.17995321216483715No Hit
CTGGATGGTGTCAGCAGCCGCACTTAAGGCCACTGTGATAGCGTCTGTAG190.17095555155659528No Hit
CTGGATGGTGTCAGCCGCCGCGGTAATACGGAGGGTCCAAGCGTTATCCG180.16195789094835342No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCT180.16195789094835342No Hit
CTGGATGGTGTCAGCCGCCGCACTTAAGGCCACTGTGATAGCGTCTGTAG170.15296023034011158No Hit
CTGGATGGTGTCAGCAGCCGCGGTAATACGGAGGGGGCTAGCGTTATTCG160.14396256973186972No Hit
CTGGATGGTGTCAGCAGCCGCGGTAATACGGAGGATGCAAGCGTTATCCG160.14396256973186972No Hit
CTGGATGGTGCCAGCAGCCGCACTTAAGGCCACTGTGATAGCGTCTGTAG150.13496490912362785No Hit
CTGGATGGTGTCAGCCGCCGCGGTAATACAGAGGATGCAAGCGTTATCCG150.13496490912362785No Hit
CTGGATGGTGTCAGCAGCCCAGTAGTCCTTCCTCTGTCTCTTATACACAT150.13496490912362785No Hit
CTGGATGGTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCG150.13496490912362785No Hit
CTGGATGGTGTCAGCAGCCCCGTAGTCCTTCCTCTGTCTCTTATACACAT140.12596724851538602No Hit
CTGGATGGTGTCAGCAGCCGCGGTAATACGAGGGGTGCAAGCGTTAATCG140.12596724851538602No Hit
CTGGATGGTGCCAGCCGCCGCGGTAATACGAGGGGTGCAAGCGTTAATCG140.12596724851538602No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATA120.10797192729890229No Hit
CTGGATGGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCG120.10797192729890229No Hit
CTGGATGGTGTCAGCCGCCGCGGTAATACGGAGGGGGCTAGCGTTATTCG120.10797192729890229No Hit
CTGGATGGTGTCAGCCGCCCCGTAGTCCTTCCTCTGTCTCTTATACACAT120.10797192729890229No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCTC157.0491496E-6295.0295
GTGCAGC108.352635E-4295.08
ATGGTGC5050.0295.05
TGTCAGC5850.0295.09
AGGCCTT157.0491496E-6295.0295
TGCCAGC4900.0295.09
TGGATGG11000.0295.02
GACTGGG108.352635E-4295.07
AGACCTT254.965841E-10295.0295
AGACCTC108.352635E-4295.0295
AGTCCTC1600.0295.0295
TGGTGTC5850.0295.06
ACTGGGA108.352635E-4295.08
TGGTGCC4950.0295.06
TGGTGCA108.352635E-4295.06
AGTCATT108.352635E-4295.0295
GAGATAA108.352635E-4295.0295
GGTGCCA4950.0295.07
GGTGTCA5850.0295.07
GGTGCAG108.352635E-4295.07
Produced by FastQC (version 0.11.5)