FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-109-5-1-3-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-109-5-1-3-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23564
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC768932.63028348327958No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT18657.914615515192666No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA18297.761840095060261No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACAGATCTG10734.553556272279749No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT7503.182821252758445No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT6882.9197080291970803No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACAGAT5192.202512306908844No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT4511.9139365133254118No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC3221.3664912578509592No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA2921.2391784077406214No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACAGATCT2711.150059412663385No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT2511.065184179256493No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACAGAT2411.0227465625530472No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT2240.9506026141571888No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACAGA2220.9421150908164998No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA2170.9208962824647768No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC1850.7850959090137497No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT1690.7171957222882364No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC1640.6959769139365133No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC1570.6662705822441012No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACAGA1550.6577830589034119No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACAGATC1470.6238329655406553No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT1460.6195892038703107No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT1320.5601765404854863No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAC1150.48803259208962824No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT1110.4710575454082499No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT1040.44135121371583774No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACAGA990.4201324053641147No Hit
ATCGGTTACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT950.4031573586827364No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAC930.3946698353420472No Hit
ATCGGTTACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACAGAT880.3734510269903242No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT870.3692072653199796No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA820.34798845696825664No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT810.3437446952979121No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG660.28008827024274313No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT620.2631132235613648No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG590.25038193855033103No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT590.25038193855033103No Hit
ATCGGTTACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA550.23340689186895264No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTACA540.22916313019860804No Hit
ATCGGTTACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAC530.22491936852826344No Hit
ATCGGTTACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTACAGAT520.22067560685791887No Hit
ATCGGTTACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA520.22067560685791887No Hit
ATCGGTTACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTA490.2079443218468851No Hit
ATCGGTTACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGA480.2037005601765405No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACA460.1952130368358513No Hit
ATCGGTTACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA450.1909692751655067No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGGCCTAGAGGAGCCT430.18248175182481752No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAC430.18248175182481752No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAC410.17399422848412832No Hit
ATCGGTTACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTACAG410.17399422848412832No Hit
ATCGGTTACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAC400.16975046681378375No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACAGA390.16550670514343915No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAC380.16126294347309456No Hit
ATCGGTTACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT380.16126294347309456No Hit
ATCGGTTACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACAG350.1485316584620608No Hit
ATCGGTTACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGT330.14004413512137157No Hit
ATCGGTTACTGGGATTAGATACCCCTATAAGAGGAGCCTGTTCTACAGAT310.1315566117806824No Hit
ATCGGTTACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACAGAT310.1315566117806824No Hit
ATCGGTTACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTACAG300.1273128501103378No Hit
ATCGGTTACTGGGATTAGATACCCCACGAACTAGAGGAGCCTGTTCTACA300.1273128501103378No Hit
ATCGGTTACTGGGATTAGATACCCCAATAGTCCTAGAGGAGCCTGTTCTA300.1273128501103378No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAAT290.12306908843999322No Hit
ATCGGTTACTGGGATTAGATACCCCTGTATAAGAGGAGCCTGTTCTACAG290.12306908843999322No Hit
ATCGGTTACTGGGATTAGATACCCCATACGACTCGCTAGAGGAGCCTGTT280.1188253267696486No Hit
ATCGGTTACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGC270.11458156509930402No Hit
ATCGGTTACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC270.11458156509930402No Hit
ATCGGTTACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTACAG270.11458156509930402No Hit
ATCGGTTACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA260.11033780342895944No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT260.11033780342895944No Hit
ATCGGTTACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTACAG260.11033780342895944No Hit
ATCGGTTACTGGGATTAGATACCCCGAGATGCCTAAGCTAGAGGAGCCTG250.10609404175861484No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGAT240.10185028008827025No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTGG23200.0295.06
TCGGTTA23400.0295.02
ATCGGTT23400.0295.01
CGGTTAC23300.0295.03
ATGGGAT108.4125507E-4294.999978
GGTGACT108.4125507E-4294.999974
TGACTGG108.4125507E-4294.999976
TCGGTGA108.4125507E-4294.999972
GGTTATG108.4125507E-4294.999974
GGTTACT23250.0294.999974
ATCGGTG108.4125507E-4294.999971
CGGTGAC108.4125507E-4294.999973
GTGACTG108.4125507E-4294.999975
GACTGGG108.4125507E-4294.999977
CGGTTAT108.4125507E-4294.999973
GTTATGG108.4125507E-4294.999975
TATGGGA108.4125507E-4294.999977
ACTGGGA23350.0294.368328
CTGGGAT23300.0294.366949
TACTGGG23250.0294.365577
Produced by FastQC (version 0.11.5)