FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-108-5-8-2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-108-5-8-2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23705
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA725030.58426492301202No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAT17307.298038388525628No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA15476.5260493566758075No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATTCCTCTGT9263.9063488715460872No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATTCCTC6972.94030795190888No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA6602.784222737819025No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA6142.590170850031639No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAT4431.8688040497785279No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG4151.7506855093862055No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT3461.4596076777051255No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA2821.1896224425226745No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATTCCTC2631.1094705758278844No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATTCCTCTG2190.9238557266399494No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA2020.8521408985446108No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATTCCT1990.8394853406454335No Hit
CAACACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTATTCCT1980.8352668213457077No Hit
CAACACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATTCCTCT1710.7213668002531112No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA1710.7213668002531112No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA1700.7171482809533853No Hit
CAACACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1670.704492723054208No Hit
CAACACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA1670.704492723054208No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA1610.6791816072558532No Hit
CAACACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATTCCT1500.6327778949588694No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA1450.6116852984602404No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA1400.5905927019616114No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAT1200.5062223159670955No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA1120.4724741615692892No Hit
CAACACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTATT1080.45560008437038596No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA1050.44294452647120863No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC910.38388525627504744No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA900.37966673697532166No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA880.37122969837587005No Hit
CAACACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATTC870.3670111790761443No Hit
CAACACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTATT860.36279265977641845No Hit
CAACACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATTCCT790.3332630246783379No Hit
CAACACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATTCCTC790.3332630246783379No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC690.29107783168108No Hit
CAACACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTATTCCTC640.269985235182451No Hit
CAACACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTATTCC590.24889263868382197No Hit
CAACACACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAT580.24467411938409617No Hit
CAACACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTATTCCTC580.24467411938409617No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAT570.2404556000843704No Hit
CAACACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTATT570.2404556000843704No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA530.2235815228854672No Hit
CAACACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAT510.2151444842860156No Hit
CAACACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT500.2109259649862898No Hit
CAACACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC480.20248892638683821No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA470.1982704070871124No Hit
CAACACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATTCCTCT450.18983336848766083No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT450.18983336848766083No Hit
CAACACACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTATTCC440.18561484918793503No Hit
CAACACACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTATTC430.18139632988820922No Hit
CAACACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA430.18139632988820922No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG420.17717781058848345No Hit
CAACACACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTT390.16452225268930604No Hit
CAACACACTGGGATTAGACACCCCACTATGCTTAGCCCTAAACCTCAACA390.16452225268930604No Hit
CAACACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTATT390.16452225268930604No Hit
CAACACACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGCC380.16030373338958026No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA360.15186669479012865No Hit
CAACACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTATTCC360.15186669479012865No Hit
CAACACACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTAT350.14764817549040285No Hit
CAACACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTATT350.14764817549040285No Hit
CAACACACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTATTCC340.14342965619067707No Hit
CAACACACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTT330.13921113689095127No Hit
CAACACACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTATTCC320.1349926175912255No Hit
CAACACACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTATT320.1349926175912255No Hit
CAACACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTATTCCTC310.13077409829149966No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT310.13077409829149966No Hit
CAACACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA310.13077409829149966No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG300.1265555789917739No Hit
CAACACACTGGGATTAGATACCCCTCCAGCCCACGAGACCTAGAGGAGCC300.1265555789917739No Hit
CAACACACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGAG300.1265555789917739No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTATTCCTC300.1265555789917739No Hit
CAACACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTATTCCT290.12233705969204808No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAT290.12233705969204808No Hit
CAACACACTGGGATTAGATACCCCCCACGAGACCTAGAGGAGCCTGTTCT290.12233705969204808No Hit
CAACACACTGGGATTAGATACCCCCGTGGACTAGAGGAGCCTGTTCTATT280.1181185403923223No Hit
CAACACACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCT280.1181185403923223No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA270.11390002109259649No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA270.11390002109259649No Hit
CAACACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA250.1054629824931449No Hit
CAACACACTGGGATTAGATACCCCCGCAACCTAGAGGAGCCTGTTCTATT250.1054629824931449No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAACA240.10124446319341911No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGG303.6379788E-12295.06
ACGCTGG303.6379788E-12295.05
GCTGGGA303.6379788E-12295.07
CACCTGG108.412888E-4295.04
CACTGGG23100.0294.999976
ACTGGGA23100.0294.999977
CTGGGAT23450.0294.3718
TGGGATT23400.0294.369669
ACACTGG23200.0294.364235
CACACTG23600.0290.04
ACACACT24650.0277.647063
CACGCTG351.2732926E-11252.857134
CAACACA29650.0231.821241
AACACAC30150.0227.487552
ACACGCT403.274181E-11221.253
TGGGATA150.002832189196.666679
ACACCTG200.0066964077147.53
AACACGC701.6189006E-9126.4285662
CAACACG1052.709021E-884.285721
AAACCTT1900.059.00000440-44
Produced by FastQC (version 0.11.5)