FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-107-5-2-6-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-107-5-2-6-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23086
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA743432.201334141904184No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAC17567.606341505674434No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA17567.606341505674434No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACAGATCTGT11895.150307545698691No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA8683.759854457246816No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA6512.8198908429351124No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT5472.3694013687949407No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACAGATC4431.9189118946547692No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA3401.4727540500736376No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACAGATCTG2941.2734990903577925No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAC2931.2691674607987524No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA2250.9746166507840249No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA2210.9572901325478645No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC2200.9529585029888245No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACAGAT2160.935631984752664No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA1810.7840249501862601No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACAGATC1600.6930607294464177No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACAGATCT1590.6887290998873776No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA1450.6280862860608161No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA1380.5977648791475353No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG1300.5631118426752144No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAC1280.5544485835571342No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA1150.4981373992896127No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACAGAT1120.4851425106124924No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA1120.4851425106124924No Hit
GCCACAACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACAGAT1020.4418262150220913No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAC1000.4331629559040111No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA960.41583643766785067No Hit
GCCACAACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAC930.4028415489907304No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA920.3985099194316902No Hit
GCCACAACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC850.3681885125184094No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA820.3551936238412891No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA710.3075456986918479No Hit
GCCACAACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA680.29455081001472755No Hit
GCCACAACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACAG660.2858875508966473No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTACA630.27289266221952696No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTACA620.26856103266048686No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACAGAT610.26422940310144677No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA610.26422940310144677No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA570.2469028848652863No Hit
GCCACAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTACA570.2469028848652863No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC560.2425712553062462No Hit
GCCACAACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTACAGATC560.2425712553062462No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT560.2425712553062462No Hit
GCCACAACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTACA490.21224984839296543No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGTGCCTAGAGGAGCC480.20791821883392533No Hit
GCCACAACTGGGATTAGATACCCCGAGCCACGAGACTAGAGGAGCCTGTT470.20358658927488524No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTACA460.1992549597158451No Hit
GCCACAACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACAGA450.194923330156805No Hit
GCCACAACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT440.19059170059776487No Hit
GCCACAACTGGGATTAGATACCCCTGACCTAGAGGAGCCTGTTCTACAGA440.19059170059776487No Hit
GCCACAACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTACAG420.18192844147968465No Hit
GCCACAACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTACAGA390.16893355280256434No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG360.155938664125444No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG360.155938664125444No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA340.14727540500736377No Hit
GCCACAACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA330.14294377544832365No Hit
GCCACAACTGGGATTAGATACCCCTGACGCTCTAGAGGAGCCTGTTCTAC310.13428051633024343No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC310.13428051633024343No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTAC310.13428051633024343No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA300.1299488867712033No Hit
GCCACAACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACAGATC290.1256172572121632No Hit
GCCACAACTGGGATTAGATACCCCAATGAGATCGAGGGCCTAGAGGAGCC280.1212856276531231No Hit
GCCACAACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACAGATC270.116953998094083No Hit
GCCACAACTGGGATTAGATACCCCCACCCTAGAGGAGCCTGTTCTACAGA270.116953998094083No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTA270.116953998094083No Hit
GCCACAACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTACAGA270.116953998094083No Hit
GCCACAACTGGGATTAGATACCCCCACAGAGAGCTAGAGGAGCCTGTTCT270.116953998094083No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA260.11262236853504287No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA260.11262236853504287No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCGTAAACCTTGATA260.11262236853504287No Hit
GCCACAACTGGGATTAGATACCCCTACCCTAGAGGAGCCTGTTCTACAGA260.11262236853504287No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT250.10829073897600278No Hit
GCCACAACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTACAGAT250.10829073897600278No Hit
GCCACAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA240.10395910941696267No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGGG22550.0295.06
ACACTGG157.1488594E-6295.04
CACTGGG108.4113894E-4295.05
TGGGATA157.1488594E-6295.09
ACTGGGT108.4113894E-4295.07
GTAAATA108.4113894E-4295.0295
GCCACAA22950.0293.714571
CAACTGG22800.0293.706155
CTGGGAT22600.0293.694678
ACTGGGA22550.0293.69187
TGGGATT22450.0293.028969
CCACAAC23450.0286.823032
CACAACT23450.0286.194033
ACAACTG23750.0281.957924
CTGGATT202.2533293E-5221.258
TGGATTA202.2533293E-5221.259
AACTGGA255.4865304E-5177.06
ACTGGAT301.1346342E-4147.57
CCACACT352.0964108E-4126.4285662
GCCACAC352.0964108E-4126.4285661
Produced by FastQC (version 0.11.5)