FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-106-6-7-5-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-106-6-7-5-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22483
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1125150.04225414757817No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG10364.607925988524663No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG9704.314370857981586No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG6162.7398478850687185No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG4892.174976649023707No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG4572.0326468887603966No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG4371.943690788595828No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG3011.33878930747676No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT2991.3298936974603033No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG2931.3032068674109327No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC2711.205355157229907No Hit
AAGCGACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATAG2120.9429346617444292No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG1940.8628741715963173No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATAC1810.8050527064893475No Hit
AAGCGACTGGGATTAGATACCCCACTATTCTTAGCCCTAAACCTCAACAG1570.6983053862918649No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG1540.6849619712671796No Hit
AAGCGACTGGGATTAGATACCCCCGCAGCAAGCGCATCGCGAACACCGTG1510.6716185562424943No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCCAAACCTCAACAG1470.6538273362095806No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG1400.6226927011519815No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGATAA1130.5026019659298137No Hit
AAGCGACTGGGATTAGATACCCCCACTATGCTTAGCCCTAAACCTCAACA1040.4625717208557577No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATAG1000.444780500822844No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCTTAAACATTGGCAA900.4003024507405595No Hit
AAGCGACTGGGATTAGATACCCCGAGAGTTTTCGGAAGATCGTGGCCCTC870.3869590357158742No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGCCCTAAATCTTGATACT870.3869590357158742No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT850.37806342569941737No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT840.3736156206911889No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCTACGCCCTAAACGATGATCG780.34692879064181825No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAGCCTCAACAG720.32024196059244764No Hit
AAGCGACTGGGATTAGATACCCCAATAATCCGGCCTGGCAACGCACGTTT680.30245074055953386No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCCAAATAG590.2624204954854779No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCACGCCCTAAACGCTGTCTG560.2490770804607926No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT550.24462927545256416No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATAG500.222390250411422No Hit
AAGCGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT490.21794244540319355No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG470.20904683538673663No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCACTCCTGT440.19570342036205132No Hit
AAGCGACTGGGATTAGATACCCCCACAGCCATCATGGTGGGCGTCGGAAA440.19570342036205132No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACAG360.16012098029622382No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCACGCCCTAAACGATGAATG350.15567317528799537No Hit
AAGCGACTGGGATTAGATACCCCGAGAGTTTTTGGAAGATCGTGGCCCTC350.15567317528799537No Hit
AAGCGACTGGGATTAGATACCCCAACACTCATCAAGATGGCCAAGTCTGG340.15122537027976693No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTACC340.15122537027976693No Hit
AAGCGACTGGGATTAGATACCCCATGCCCTGCGCATCCAGTTGGCGACGT330.14677756527153848No Hit
AAGCGACTGGGATTAGATACCCCACTGTGCGCTCCCCTCTCATTCACTTC330.14677756527153848No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG320.14232976026331007No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCACTAAACTCTAATAG320.14232976026331007No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG310.13788195525508162No Hit
AAGCGACTGGGATTAGATACCCCCGCAGCAAGCGCATCACGAACACCGTG300.13343415024685318No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTGGCCCTAAACGATGAGTG280.1245385402303963No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCACGCCCTAAACGTTCCTCT280.1245385402303963No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTGGCCCTCAACGTTGCTCG270.12009073522216787No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATATC270.12009073522216787No Hit
AAGCGACTGGGATTAGATACCCGGGTAGTCTCAGACGTAAACGATGGACG270.12009073522216787No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCACTC260.11564293021393943No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGCCCTAAACGTTGAGCA260.11564293021393943No Hit
AAGCGATGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT250.111195125205711No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGCTGTAAACAATGGATA240.10674732019748255No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGCCGTAAACGATGGATA240.10674732019748255No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACAG240.10674732019748255No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTGGCCCTAAACGTTGCTCG240.10674732019748255No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACAG230.10229951518925409No Hit
AAGCGACTGGGATTAGATACCCCCGAACGATATCCAACTAGACTATTGCT230.10229951518925409No Hit
AAGCGACTGGGATTAGATACCCCTCACCGTGGCTTTCGAACGAATAACCC230.10229951518925409No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTG22350.0295.000033
CGACTGG22350.0295.000034
CTGGGAT22300.0295.07
GGGATTG108.4099284E-4295.09
TGGGATT22300.0295.08
AGCGACT22400.0294.341522
GACTGGG22350.0294.340065
ACTGGGA22350.0294.340066
AAGCGAC22550.0293.69181
GGGATTA22350.0293.68019
ATGATCA200.0066940635147.5295
ACTAGAA352.0956868E-4126.428566295
TGAGAAA257.788469E-659.00000455-59
CGCGAAG257.788469E-659.000004195-199
TGGTTTA257.788469E-659.000004175-179
TGTTGAA150.004370230759.00000455-59
AACGCGA303.315272E-759.000004190-194
GTATGCT150.004370230759.000004115-119
TGGTTCA150.004370230759.000004175-179
TCCCATC257.788469E-659.00000450-54
Produced by FastQC (version 0.11.5)