FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-105-5-2-6-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-105-5-2-6-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22877
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA767033.52712331162302No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA17647.710801241421515No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAT15966.97643921842899No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATTCCTCTGT10414.550421821042969No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA7993.4925908117323075No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA6382.788827206364471No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATTCCTC5582.439131004939459No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT4091.7878218297853739No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATTCCTCTG3531.5430344887878655No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA3231.4118984132534862No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC2831.23705031254098No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAT2781.2151942999519167No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA2711.1845958823272282No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATTCCT2451.0709446168640993No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA2170.948550946365345No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA1630.7125060104034621No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1600.699392402850024No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA1550.6775363902609608No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG1550.6775363902609608No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA1430.625081960047209No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAT1390.6075971499759584No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA1350.5901123399047078No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATTCCTC1340.5857411373868951No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA1230.537657909690956No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA1220.5332867071731433No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATTCCTCT1220.5332867071731433No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA1030.450233859334703No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA860.37592341653188793No Hit
GCCACAACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC820.35843860646063735No Hit
GCCACAACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATTC820.35843860646063735No Hit
GCCACAACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTATTCCTC760.33221139135376143No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA760.33221139135376143No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA700.3059841762468855No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAT650.2841281636578223No Hit
GCCACAACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT650.2841281636578223No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG530.23167373344407044No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTATT500.21856012589063253No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTATT470.20544651833719457No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT470.20544651833719457No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATTCCT440.1923329107837566No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATTCCT440.1923329107837566No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC420.18359050574813132No Hit
GCCACAACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTT420.18359050574813132No Hit
GCCACAACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTATTCCT420.18359050574813132No Hit
GCCACAACTGGGATTAGATACCCCACGGGACTAGAGGAGCCTGTTCTATT410.17921930323031868No Hit
GCCACAACTGGGATTAGATACCCCTGCTGCAACTAGAGGAGCCTGTTCTA410.17921930323031868No Hit
GCCACAACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT390.17047689819469336No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC380.16610569567688072No Hit
GCCACAACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTATTCCTC380.16610569567688072No Hit
GCCACAACTGGGATTAGATACCCCGACGACTAGAGGAGCCTGTTCTATTC370.16173449315906807No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA370.16173449315906807No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTA350.15299208812344275No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATG350.15299208812344275No Hit
GCCACAACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTATTCCT350.15299208812344275No Hit
GCCACAACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAT340.1486208856056301No Hit
GCCACAACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTATTC340.1486208856056301No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCT330.14424968308781747No Hit
GCCACAACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTATTCCTC320.1398784805700048No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA320.1398784805700048No Hit
GCCACAACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATTCCTC320.1398784805700048No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA310.13550727805219215No Hit
GCCACAACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTATTCCTC300.1311360755343795No Hit
GCCACAACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTAT300.1311360755343795No Hit
GCCACAACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA290.12676487301656686No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTATT290.12676487301656686No Hit
GCCACAACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTATT280.1223936704987542No Hit
GCCACAACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTATTCCTC270.11802246798094154No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT270.11802246798094154No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG250.10928006294531627No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTAACA250.10928006294531627No Hit
GCCACAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA240.10490886042750361No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA230.10053765790969096No Hit
GCCACAACTGGGATTAGATACCCCTGTCAGACTAGAGGAGCCTGTTCTAT230.10053765790969096No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGATT108.4109115E-4295.08
GCCACAA22800.0294.353061
AACTGGG22500.0294.344456
CTGGGAT22500.0294.344458
ACTGGGA22500.0294.344457
CAACTGG22650.0293.697575
TGGGATT22550.0293.69189
CCACAAC23200.0288.006472
CACAACT23600.0283.1253
ACAACTG23800.0280.126044
ACTGGAT150.0028315245196.666677
TGGATTA200.006694842147.59
AAACTTT600.059.00000440-44
GGCGTTG150.0043704359.000004110-114
CCCGGTA150.0043704359.00000420-24
CGCCCAC150.0043704359.000004175-179
CCCGGGT150.0043704359.00000420-24
GCCTAGC2150.059.00000430-34
GTTCTAG150.0043704359.000004140-144
TAGCTTA452.5465852E-1159.00000490-94
Produced by FastQC (version 0.11.5)