FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-104-4-5-7-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-104-4-5-7-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22882
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA361015.776592955161261No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC22589.86801852984879No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGAA21809.527139236080762No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGCTGA14786.459225592168518No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA7223.1553185910322523No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGCTG6112.6702211345162135No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC5912.5828161873962063No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA5832.5478542085482037No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGCTGAGA5732.5041517349882003No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT4191.8311336421641466No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGCTGA3891.700026221484136No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGCTGAG3551.5514378113801242No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT3471.5164758325321213No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT3341.4596626169041167No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA2881.2586312385281007No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGCTG2691.175596538764094No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGC2601.1362643125600909No Hit
CTGGATGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGCT2120.9264924394720742No Hit
CTGGATGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGCTG1910.8347172449960668No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT1900.8303469976400665No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT1600.699239576960056No Hit
CTGGATGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAG1480.6467966086880517No Hit
CTGGATGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGCTGA1440.6293156192640503No Hit
CTGGATGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT1410.6162048771960493No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAG1340.5856131457040469No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT1340.5856131457040469No Hit
CTGGATGACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTA1240.5419106721440433No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGCTG1160.5069486932960405No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC1120.48946770387203914No Hit
CTGGATGACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT1020.44576523031203563No Hit
CTGGATGACTGGGATTAGATACCCCCCGAGACCTAGAGGAGCCTGTTCTA1000.43702473560003496No Hit
CTGGATGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC950.41517349882003324No Hit
CTGGATGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAG860.37584127261603006No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT840.36710077790402934No Hit
CTGGATGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGCTGA830.362730530548029No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT770.33650904641202695No Hit
CTGGATGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGCT760.33213879905602656No Hit
CTGGATGACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAGCTG730.3190280569880255No Hit
CTGGATGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGCTGA700.3059173149200245No Hit
CTGGATGACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTA690.30154706756402416No Hit
CTGGATGACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTA690.30154706756402416No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAG660.2884363254960231No Hit
CTGGATGACTGGGATTAGATACCCCTGACCGACTAGAGGAGCCTGTTCTA650.2840660781400227No Hit
CTGGATGACTGGGATTAGATACCCCATGTGCCTCTAGAGGAGCCTGTTCT640.2796958307840224No Hit
CTGGATGACTGGGATTAGATACCCCCACTCCGCTAGAGGAGCCTGTTCTA630.27532558342802205No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGCTGA600.26221484136002093No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC590.2578445940040206No Hit
CTGGATGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGCTGA580.25347434664802027No Hit
CTGGATGACTGGGATTAGATACCCCGCAGACCTAGAGGAGCCTGTTCTAG570.2491040992920199No Hit
CTGGATGACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAG570.2491040992920199No Hit
CTGGATGACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGCT550.24036360458001924No Hit
CTGGATGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCC550.24036360458001924No Hit
CTGGATGACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCT550.24036360458001924No Hit
CTGGATGACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGCTG540.23599335722401887No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT530.23162310986801854No Hit
CTGGATGACTGGGATTAGATACCCCCACGGGACCTAGAGGAGCCTGTTCT530.23162310986801854No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT520.2272528625120182No Hit
CTGGATGACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGCTGA510.22288261515601782No Hit
CTGGATGACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGA500.21851236780001748No Hit
CTGGATGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAG490.21414212044401715No Hit
CTGGATGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGCT490.21414212044401715No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCT460.20103137837601606No Hit
CTGGATGACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTT450.19666113102001573No Hit
CTGGATGACTGGGATTAGATACCCCAACCCACCTAGAGGAGCCTGTTCTA440.1922908836640154No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAG440.1922908836640154No Hit
CTGGATGACTGGGATTAGATACCCCCGACCAACCTAGAGGAGCCTGTTCT390.17043964688401364No Hit
CTGGATGACTGGGATTAGATACCCCAATGTCAGACTAGAGGAGCCTGTTC380.16606939952801328No Hit
CTGGATGACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA380.16606939952801328No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT370.16169915217201292No Hit
CTGGATGACTGGGATTAGATACCCCGTAGTCCTAGAGGAGCCTGTTCTAG370.16169915217201292No Hit
CTGGATGACTGGGATTAGATACCCCAATCTAGAGGAGCCTGTTCTAGCTG350.15295865746001225No Hit
CTGGATGACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCTA340.1485884101040119No Hit
CTGGATGACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAGCT330.14421816274801155No Hit
CTGGATGACTGGGATTAGATACCCCCACGAATAGAGGAGCCTGTTCTAGC330.14421816274801155No Hit
CTGGATGACTGGGATTAGATACCCCGTCTCTTATACCATCTGAGCTAGAG330.14421816274801155No Hit
CTGGATGACTGGGATTAGATACCCCACGAACTAGAGGAGCCTGTTCTAGC310.13547766803601083No Hit
CTGGATGACTGGGATTAGATACCCCGACGAACCTAGAGGAGCCTGTTCTA300.13110742068001047No Hit
CTGGATGACTGGGATTAGATACCCCGGACTAGAGGAGCCTGTTCTAGCTG280.12236692596800978No Hit
CTGGATGACTGGGATTAGATACCCCGAAACCTAGAGGAGCCTGTTCTAGC270.11799667861200944No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATA260.1136264312560091No Hit
CTGGATGACTGGGATTAGATACCCCCGTGGACTAGAGGAGCCTGTTCTAG260.1136264312560091No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT250.10925618390000874No Hit
CTGGATGACTGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG240.10488593654400839No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT240.10488593654400839No Hit
CTGGATGACTGGGATTAGATACCCCTACGCTAGAGGAGCCTGTTCTAGCT230.10051568918800803No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAG230.10051568918800803No Hit
CTGGATGACTGGGATTAGATACCCCCGCACTAGAGGAGCCTGTTCTAGCT230.10051568918800803No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGATG22750.0294.984681
GACTGGG22450.0294.984687
GGATGAC22750.0294.336363
CTGGGAT22450.0294.32779
ACTGGGA22450.0294.32778
TGACTGG22650.0293.68236
TGGATGA22900.0293.052462
ATGACTG22700.0293.035435
GATGACT22950.0291.128664
CTGGATT150.0028319652196.656459
GACTGGA150.0028319652196.656457
ACTGGAT200.0066958815147.492348
AGGGCCT650.059.12631635-39
GGCCCTA257.691122E-659.12631635-39
GAGGGCC650.059.12631635-39
GGGCCTA600.059.12631235-39
GCCTAGC501.8189894E-1259.04862230-34
CTTAGCC2550.059.04862230-34
TGCCTGG257.7513705E-659.04862230-34
CTGGCCC257.7513705E-659.04862230-34
Produced by FastQC (version 0.11.5)