FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-103-4-4-1-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-103-4-4-1-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22710
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGC301613.280493174812857No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG294612.972258916776749No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGAACT251711.08322324966975No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGCTGAGA13946.1382650814619115No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAGC7513.306913254073095No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG5402.3778071334214No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGCTGAGA5022.2104799647732274No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGCTGAG4642.043152796125055No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAGC4341.9110523998238662No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAG3881.7084984588287098No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGCTGAGAAC3731.6424482606781154No Hit
GGTACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGCTGAGAA3641.6028181417877585No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAG2921.2857771906649054No Hit
GGTACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGCTGAG2721.197710259797446No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT2621.1536767943637165No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC2511.1052399823866137No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT2250.9907529722589168No Hit
GGTACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGCT2180.959929546455306No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG2020.8894760017613387No Hit
GGTACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGCTGAG1690.7441655658300308No Hit
GGTACACTGGGATTAGATACCCCGTAGTCCTAGAGGAGCCTGTTCTAGCT1680.7397622192866579No Hit
GGTACACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAGCTGA1660.7309555261999119No Hit
GGTACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGCTGA1650.726552179656539No Hit
GGTACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGCTGAG1490.6560986349625716No Hit
GGTACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG1480.6516952884191985No Hit
GGTACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAGCT1470.6472919418758256No Hit
GGTACACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTTCT1440.6340819022457067No Hit
GGTACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGCTG1270.5592250110083663No Hit
GGTACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGCTGAGA1190.5239982386613826No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG1170.5151915455746366No Hit
GGTACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAGCT980.4315279612505504No Hit
GGTACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG890.39189784236019376No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGCT880.38749449581682077No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAG880.38749449581682077No Hit
GGTACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGCTGAGA870.3830911492734478No Hit
GGTACACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCTG850.3742844561867019No Hit
GGTACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT840.36988110964332893No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG830.36547776309995594No Hit
GGTACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGCTGAG800.3522677234698371No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAGC790.3478643769264641No Hit
GGTACACTGGGATTAGATACCCCAGACGCTGCTAGAGGAGCCTGTTCTAG780.34346103038309117No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG760.3346543372963452No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAGCT760.3346543372963452No Hit
GGTACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGCTGAGA750.33025099075297226No Hit
GGTACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAGCT740.3258476442095993No Hit
GGTACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGCTGAGA740.3258476442095993No Hit
GGTACACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAGCTG700.30823425803610743No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT690.3038309114927345No Hit
GGTACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGCTGAGA670.2950242184059885No Hit
GGTACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGCTGA670.2950242184059885No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTAGCT640.2818141787758697No Hit
GGTACACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTAGCTG630.2774108322324967No Hit
GGTACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAG610.26860413914575076No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG590.25979744605900484No Hit
GGTACACTGGGATTAGATACCCCTAGTTGACTAGAGGAGCCTGTTCTAGC580.25539409951563186No Hit
GGTACACTGGGATTAGATACCCCACTAGACCTAGAGGAGCCTGTTCTAGC570.2509907529722589No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAG560.24658740642888596No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG540.23778071334214002No Hit
GGTACACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCTA530.23337736679876708No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG500.22016732716864817No Hit
GGTACACTGGGATTAGATACCCCGGCCCACGAGACCTAGAGGAGCCTGTT490.2157639806252752No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG480.21136063408190223No Hit
GGTACACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAGCTG470.2069572875385293No Hit
GGTACACTGGGATTAGATACCCCTGACGCTCTAGAGGAGCCTGTTCTAGC450.19815059445178335No Hit
GGTACACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAGCTGAG450.19815059445178335No Hit
GGTACACTGGGATTAGATACCCCTATAAAGGGCTAGAGGAGCCTGTTCTA420.18494055482166447No Hit
GGTACACTGGGATTAGATACCCCCGACACCTAGAGGAGCCTGTTCTAGCT410.1805372082782915No Hit
GGTACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGCTGAGA380.1673271686481726No Hit
GGTACACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTAGCTGAG380.1673271686481726No Hit
GGTACACTGGGATTAGATACCCCGAGCCTACGAGACCTAGAGGAGCCTGT370.16292382210479964No Hit
GGTACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAGC370.16292382210479964No Hit
GGTACACTGGGATTAGATACCCCTACCCTAGAGGAGCCTGTTCTAGCTGA350.15411712901805372No Hit
GGTACACTGGGATTAGATACCCCGCACGGATCTAGAGGAGCCTGTTCTAG340.14971378247468076No Hit
GGTACACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTAGCTG340.14971378247468076No Hit
GGTACACTGGGATTAGATACCCCCAGAACCTAGAGGAGCCTGTTCTAGCT300.13210039630118892No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG290.12769704975781593No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG290.12769704975781593No Hit
GGTACACTGGGATTAGATACCCCCATCTAGAGGAGCCTGTTCTAGCTGAG280.12329370321444298No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT270.11889035667107001No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG270.11889035667107001No Hit
GGTACACTGGGATTAGATACCCCCGCTAGAGGAGCCTGTTCTAGCTGAGA260.11448701012769705No Hit
GGTACACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTAGCTGA240.10568031704095111No Hit
GGTACACTGGGATAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGCT240.10568031704095111No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAGCT230.10127697049757817No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATAG157.147428E-6295.09
TGGGATT21950.0295.08
CTGGATT206.053051E-8294.999977
CACTGGG22250.0294.337075
CTGGGAT22150.0294.334087
GGTACAC22800.0293.706121
ACACTGG22500.0293.688874
ACTGGGA22250.0293.674166
GGGATTA22050.0293.662149
GTACACT22850.0291.772432
TACACTG22950.0289.21573
TGGATTA251.8421088E-7236.08
CACTGGA304.5709749E-7196.666675
ACTGGAT304.5709749E-7196.666676
TGGGATA301.13440794E-4147.58
GCCTCAG900.059.000004115-119
CCCAGAG452.5465852E-1159.00000420-24
ACCCCGG900.059.00000415-19
ACCCCCT452.5465852E-1159.00000415-19
CCCCCTA452.5465852E-1159.00000420-24
Produced by FastQC (version 0.11.5)