FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-102-5-4-1-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-102-5-4-1-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22036
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG521523.66581956797967No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGC222710.106189871119986No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGAACT18138.227445997458705No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG10204.628789253948085No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGCTGAGA9844.465420221455799No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAGC5162.3416227990560903No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC4462.0239607914322018No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG4261.9332002178253767No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGCTGAGA3901.7698311853330915No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT3621.642766382283536No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAGC3191.4476311490288618No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT3121.415864948266473No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGCTGAG3111.4113269195861318No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGCTGAGAAC2621.1889635142494102No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAG2501.1345071700853149No Hit
GGTACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGCTGAGAA2311.048284625158831No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG2060.9348339081502994No Hit
GGTACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGCTGAG2000.9076057360682519No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT1820.8259212198221092No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAG1750.7941550190597205No Hit
GGTACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGCT1640.7442367035759666No Hit
GGTACACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAGCTGA1260.5717916137229987No Hit
GGTACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGCTGAG1210.5491014703212924No Hit
GGTACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGCTGA1110.5037211835178799No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG1110.5037211835178799No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG1090.49464512615719736No Hit
GGTACACTGGGATTAGATACCCCGTAGTCCTAGAGGAGCCTGTTCTAGCT960.435650753312761No Hit
GGTACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG920.4174986385913959No Hit
GGTACACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTTCT890.40388455255037214No Hit
GGTACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGCTGAG860.3902704665093483No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG830.37665638046832456No Hit
GGTACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAGCT820.3721183517879833No Hit
GGTACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGCTGAGA820.3721183517879833No Hit
GGTACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGCTG810.367580323107642No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG800.3630422944273008No Hit
GGTACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGCTGAGA770.34942820838627703No Hit
GGTACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAGCT750.34035215102559446No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG690.31312397894354693No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAG680.3085859502632056No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG660.29950989290252317No Hit
GGTACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC640.29043383554184066No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGCT620.2813577781811581No Hit
GGTACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGCTGAGA610.27681974950081684No Hit
GGTACACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCTG590.2677436921401343No Hit
GGTACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGCTGAGA590.2677436921401343No Hit
GGTACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG580.26320566345979307No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAGC570.2586676347794518No Hit
GGTACACTGGGATTAGATACCCCAGACGCTGCTAGAGGAGCCTGTTCTAG560.25412960609911056No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG500.22690143401706298No Hit
GGTACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGCTGAGA490.22236340533672172No Hit
GGTACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGCTGA490.22236340533672172No Hit
GGTACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAGCT480.2178253766563805No Hit
GGTACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGCTGAG480.2178253766563805No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAGCT470.2132873479760392No Hit
GGTACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAG460.20874931929569795No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTAGCT420.1905972045743329No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG410.18605917589399165No Hit
GGTACACTGGGATTAGATACCCCGGCCCACGAGACCTAGAGGAGCCTGTT410.18605917589399165No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT390.17698311853330911No Hit
GGTACACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTAGCTG390.17698311853330911No Hit
GGTACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT380.1724450898529679No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAG380.1724450898529679No Hit
GGTACACTGGGATTAGATACCCCACTAGACCTAGAGGAGCCTGTTCTAGC370.1679070611726266No Hit
GGTACACTGGGATTAGATACCCCTGACGCTCTAGAGGAGCCTGTTCTAGC360.16336903249228535No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG350.1588310038119441No Hit
GGTACACTGGGATTAGATACCCCTAGTTGACTAGAGGAGCCTGTTCTAGC350.1588310038119441No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT340.1542929751316028No Hit
GGTACACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAGCTG310.14067888909057905No Hit
GGTACACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAGCTG300.1361408604102378No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG300.1361408604102378No Hit
GGTACACTGGGATTAGATACCCCTATAAAGGGCTAGAGGAGCCTGTTCTA300.1361408604102378No Hit
GGTACACTGGGATTAGATACCCCCGCTAGAGGAGCCTGTTCTAGCTGAGA290.13160283172989654No Hit
GGTACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAGC290.13160283172989654No Hit
GGTACACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTAGCTGAG290.13160283172989654No Hit
GGTACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGCTGAGA280.12706480304955528No Hit
GGTACACTGGGATTAGATACCCCTACCCTAGAGGAGCCTGTTCTAGCTGA280.12706480304955528No Hit
GGTACACTGGGATTAGATACCCCGCACGGATCTAGAGGAGCCTGTTCTAG260.11798874568887274No Hit
GGTACACTGGGATTAGATACCCCCGACACCTAGAGGAGCCTGTTCTAGCT250.11345071700853149No Hit
GGTACACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAGCTGAG240.10891268832819025No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGGG21500.0295.05
ACTGGGA21500.0295.06
TACACGG108.408782E-4294.999973
CTGGATT157.144423E-6294.999977
TGGATTA157.144423E-6294.999978
GGTACAC21950.0293.656041
ACACTGG21800.0292.970154
CTGGGAT21600.0292.95147
TGGGATT21550.0292.946668
TACACTG21950.0291.64013
GGGATTA21700.0290.921669
GTACACT22050.0290.317472
ACACGGG150.0028308097196.666664
CGGGATT150.0028308097196.666667
CACTGGA255.4831355E-5177.05
ACGGGAT200.0066931555147.499986
CTAGCAA200.0066931555147.49998295
ACTGGAT352.0951184E-4126.4285666
ACGCTTA452.5465852E-1159.00000490-94
CCCAGTA257.787434E-659.00000420-24
Produced by FastQC (version 0.11.5)