FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-101-4-7-5-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-101-4-7-5-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22201
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG433119.508130264402503No Hit
AAGCGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTATA238710.751767938381155No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATAAGCACTGT19238.661771992252602No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG9154.121435971352642No Hit
AAGCGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATAAGCAC7443.351200396378542No Hit
AAGCGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATAAGCACTG5602.5224089004999772No Hit
AAGCGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATAAGCA5412.4368271699473No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC4211.8963109769830189No Hit
AAGCGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATAAGCAC4071.8332507544705192No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTATA3791.7071303094455204No Hit
AAGCGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATAAG3741.684608801405342No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG3591.617044277284807No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATAAGCACT3471.5629926579883788No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT3281.4774109274357012No Hit
AAGCGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAT2741.2341786406017747No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTATA2551.148596910049097No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAT2060.9278861312553489No Hit
AAGCGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTATAA1920.8648259087428494No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG1880.8468087023107067No Hit
AAGCGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTATA1880.8468087023107067No Hit
AAGCGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATAAGCA1760.7927570830142786No Hit
AAGCGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTATAA1660.7477140669339218No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG1630.7342011621098149No Hit
AAGCGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTATAAGC1560.7026710508535652No Hit
AAGCGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTATAAGC1400.6306022251249943No Hit
AAGCGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATAAGCA1320.594567812260709No Hit
AAGCGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTATAAGCAC1280.5765506058285663No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAT1220.5495247961803522No Hit
AAGCGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTATAAGCAC1180.5315075897482096No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG1030.46394306562767446No Hit
AAGCGACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTATA980.44142155758749607No Hit
AAGCGACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCTGT940.42340435115535335No Hit
AAGCGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTATAA900.4053871447232107No Hit
AAGCGACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTATA880.3963785415071393No Hit
AAGCGACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTATAAGCA860.387369938291068No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG840.37836133507499664No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT800.3603441286428539No Hit
AAGCGACTGGGATTAGATACCCCATAATCCTAGAGGAGCCTGTTCTATAA790.35583982703481826No Hit
AAGCGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTATAA780.3513355254267826No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT760.3423269222107112No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG760.3423269222107112No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG740.3333183189946399No Hit
AAGCGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG740.3333183189946399No Hit
AAGCGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTATAAGC740.3333183189946399No Hit
AAGCGACTGGGATTAGATACCCCGTAGTCCTAGAGGAGCCTGTTCTATAA720.32430971577856854No Hit
AAGCGACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTATAAG710.3198054141705329No Hit
AAGCGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTATAAGCAC700.31530111256249715No Hit
AAGCGACTGGGATTAGATACCCCAGTAGCTAGAGGAGCCTGTTCTATAAG670.30178820773839016No Hit
AAGCGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTATAA670.30178820773839016No Hit
AAGCGACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTATAAGCAC650.2927796045223188No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG570.25674519165803344No Hit
AAGCGACTGGGATTAGATACCCCCGCCACCTAGAGGAGCCTGTTCTATAA560.2522408900499977No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG560.2522408900499977No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGCCGTAAACGATGGATA520.23422368361785506No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG510.22971938200981937No Hit
AAGCGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATAAGCAC510.22971938200981937No Hit
AAGCGACTGGGATTAGATACCCCTGCGTGCCGCGACTAGAGGAGCCTGTT470.21170217557767668No Hit
AAGCGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTATAAGCAC460.20719787396964098No Hit
AAGCGACTGGGATTAGATACCCCTACCTAGAGGAGCCTGTTCTATAAGCA450.20269357236160535No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG440.19818927075356965No Hit
AAGCGACTGGGATTAGATACCCCCGTGGACTAGAGGAGCCTGTTCTATAA440.19818927075356965No Hit
AAGCGACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTATA430.193684969145534No Hit
AAGCGACTGGGATTAGATACCCCTGTCAGACTAGAGGAGCCTGTTCTATA410.18467636592946265No Hit
AAGCGACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTATA410.18467636592946265No Hit
AAGCGACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATAAGCACT400.18017206432142696No Hit
AAGCGACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTATA380.1711634611053556No Hit
AAGCGACTGGGATTAGATACCCCGTGTAGTCCCTAGAGGAGCCTGTTCTA370.16665915949731994No Hit
AAGCGACTGGGATTAGATACCCCTTCTAGAGGAGCCTGTTCTATAAGCAC370.16665915949731994No Hit
AAGCGACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTATAAG350.15765055628124858No Hit
AAGCGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTATAAGC350.15765055628124858No Hit
AAGCGACTGGGATTAGATACCCCAACAACCTAGAGGAGCCTGTTCTATAA340.1531462546732129No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG340.1531462546732129No Hit
AAGCGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTATAAGC330.14864195306517725No Hit
AAGCGACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCTA320.14413765145714158No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCTTAGAGGAGCCTGTTCTATA320.14413765145714158No Hit
AAGCGACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTTC310.13963334984910591No Hit
AAGCGACTGGGATTAGATACCCCTGACCTAGAGGAGCCTGTTCTATAAGC310.13963334984910591No Hit
AAGCGACTGGGATTAGATACCCCAATCCTCTAGAGGAGCCTGTTCTATAA300.13512904824107022No Hit
AAGCGACTGGGATTAGATACCCCGGACACCTCTAGAGGAGCCTGTTCTAT290.13062474663303453No Hit
AAGCGACTGGGATTAGATACCCCGAGATCTAGAGGAGCCTGTTCTATAAG290.13062474663303453No Hit
AAGCGACTGGGATTAGATACCCCCTCCCCTATTTCCCCATTTCAGCCTAG290.13062474663303453No Hit
AAGCGACTGGGATTAGATACCCCCACGAACCTAGAGGAGCCTGTTCTATA270.1216161434169632No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT260.11711184180892753No Hit
AAGCGACTGGGATTAGATACCCCGAGATGCCTAAGCTAGAGGAGCCTGTT260.11711184180892753No Hit
AAGCGACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTATAAGC260.11711184180892753No Hit
AAGCGACTGGGATTAGATACCCCCAGTAGAGGAGCCTGTTCTATAAGCAC250.11260754020089186No Hit
AAGCGACTGGGATTAGATACCCCCGAGAAGACCTAGAGGAGCCTGTTCTA250.11260754020089186No Hit
AAGCGACTGGGATTAGATACCCCCGTCTAGAGGAGCCTGTTCTATAAGCA240.10810323859285617No Hit
AAGCGACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTATAAGC230.10359893698482049No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTCAACAG230.10359893698482049No Hit
AAGCGACTGGGATTAGATACCCCAAGATCTAGAGGAGCCTGTTCTATAAG230.10359893698482049No Hit
AAGCGACTGGGATTAGATACCCCGCTCTAAACCCGACCTGAGCCAGCTAG230.10359893698482049No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG230.10359893698482049No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGAT21950.0295.07
GACTGGG22050.0295.05
CGACTGG22150.0294.334084
ACTGGGA22050.0294.331056
GGGATTA21950.0294.328039
TGGGATT21950.0294.328038
AGCGACT22250.0293.674162
GCGACTG22200.0293.671173
AAGCGAC22500.0291.066681
TCAAGGA200.006693563147.5295
ATCCTCT150.00437010359.00000425-29
CCGTGTA150.00437010359.00000420-24
CCCAGAA150.00437010359.00000470-74
TGTCCCT150.00437010359.000004155-159
TGCCGGC150.00437010359.000004205-209
TAGCTGA150.00437010359.000004145-149
CGCCAGG1250.059.00000465-69
CCAGACC150.00437010359.00000420-24
GTCGAAT150.00437010359.00000440-44
TAGTCCC600.059.00000425-29
Produced by FastQC (version 0.11.5)