FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-100-5-5-7-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-100-5-5-7-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21856
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC647829.639458272327968No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA19779.045571010248903No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAACTTCGCC13766.295754026354319No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT12345.646046852122987No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAACTTC6332.8962298682284042No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA5422.4798682284040994No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT5182.3700585651537334No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT5002.287701317715959No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA3441.5739385065885798No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAACTT3431.569363103953148No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC2891.3222913616398242No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT2841.2994143484626648No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT2541.1621522693997073No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAACTTC2160.9882869692532943No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAACTTCGC2040.9333821376281113No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAACTT1980.9059297218155198No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT1820.8327232796486091No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT1740.7961200585651538No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAAC1650.7549414348462665No Hit
CTGGATGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAACTTC1650.7549414348462665No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC1510.6908857979502196No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAACTTCG1470.672584187408492No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC1320.6039531478770132No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT1150.5261713030746706No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT1120.5124450951683748No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT1050.4804172767203514No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1040.4758418740849195No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT1030.4712664714494876No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC920.42093704245973645No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT790.3614568081991215No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT790.3614568081991215No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT730.33400439238653No Hit
CTGGATGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAA720.32942898975109813No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT720.32942898975109813No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAAC700.32027818448023426No Hit
CTGGATGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAACT700.32027818448023426No Hit
CTGGATGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT660.3019765739385066No Hit
CTGGATGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAACT660.3019765739385066No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT630.2882503660322108No Hit
CTGGATGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC600.2745241581259151No Hit
CTGGATGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAA590.2699487554904832No Hit
CTGGATGACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTA530.24249633967789166No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT510.23334553440702782No Hit
CTGGATGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAACT490.22419472913616398No Hit
CTGGATGACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA490.22419472913616398No Hit
CTGGATGACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAAC470.21504392386530016No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAA460.21046852122986823No Hit
CTGGATGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAA450.20589311859443632No Hit
CTGGATGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAACT440.20131771595900438No Hit
CTGGATGACTGGGATTAGATACCCCCGCACCCTAGAGGAGCCTGTTCTAA430.19674231332357248No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTC420.19216691068814057No Hit
CTGGATGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAACTTC410.18759150805270863No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAACTT400.18301610541727673No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG400.18301610541727673No Hit
CTGGATGACTGGGATTAGATACCCCGACGAACCTAGAGGAGCCTGTTCTA390.17844070278184482No Hit
CTGGATGACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCC380.17386530014641288No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA370.16928989751098097No Hit
CTGGATGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAACTTC360.16471449487554907No Hit
CTGGATGACTGGGATTAGATACCCCGAGTAGCCCTAGAGGAGCCTGTTCT350.16013909224011713No Hit
CTGGATGACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT350.16013909224011713No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG350.16013909224011713No Hit
CTGGATGACTGGGATTAGATACCCCCGGACCTAGAGGAGCCTGTTCTAAC330.1509882869692533No Hit
CTGGATGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT330.1509882869692533No Hit
CTGGATGACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTA320.14641288433382138No Hit
CTGGATGACTGGGATTAGATACCCCACGAACCTAGAGGAGCCTGTTCTAA310.14183748169838947No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC310.14183748169838947No Hit
CTGGATGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAACTT300.13726207906295754No Hit
CTGGATGACTGGGATTAGATACCCCCACGAACCTAGAGGAGCCTGTTCTA290.13268667642752563No Hit
CTGGATGACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCT290.13268667642752563No Hit
CTGGATGACTGGGATTAGATACCCCAATGTCAGACTAGAGGAGCCTGTTC280.1281112737920937No Hit
CTGGATGACTGGGATTAGATACCCCTGTTATTTCCTAGAGGAGCCTGTTC280.1281112737920937No Hit
CTGGATGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAACTTC270.12353587115666179No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAAT270.12353587115666179No Hit
CTGGATGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA250.11438506588579796No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAACTTC250.11438506588579796No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAA250.11438506588579796No Hit
CTGGATGACTGGGATTAGATACCCCCCTCCGACAATCGCGATTCGAACTG250.11438506588579796No Hit
CTGGATGACTGGGATTAGATACCCCCGCAACCTAGAGGAGCCTGTTCTAA240.10980966325036604No Hit
CTGGATGACTGGGATTAGATACCCCGCTGCTAGAGGAGCCTGTTCTAACT240.10980966325036604No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTCAAC230.10523426061493411No Hit
CTGGATGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAACTTC220.10065885797950219No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAAT220.10065885797950219No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGGG108.408388E-4295.06
GATGGGA108.408388E-4295.07
ATGGGAT108.408388E-4295.08
GGATGAC21650.0294.318733
ATGACTG21450.0294.312355
TGACTGG21450.0294.312356
GACTGGG21400.0294.310737
CTGGATG21800.0293.64681
CTGGGAT21450.0293.62479
ACTGGGA21450.0293.62478
TGGATGA21850.0292.974822
GATGACT21650.0292.956124
GGATGAT150.0028306777196.666663
AGCATAA600.001785139273.74999295
ATCTTGA257.787077E-659.00000440-44
CTTTACC257.787077E-659.00000455-59
TTACTTT257.787077E-659.00000450-54
GGACCTG7100.059.000004105-109
AGGACCT7100.059.000004105-109
GTATCCG257.787077E-659.00000465-69
Produced by FastQC (version 0.11.5)