FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-10-5-1-3-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-10-5-1-3-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11496
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC290925.304453723034097No Hit
ATCGGTTACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA10048.73347251217815No Hit
ATCGGTTACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCTAGCTG7596.602296450939457No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT6315.488865692414753No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA3633.157620041753653No Hit
ATCGGTTACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCTAG3392.9488517745302714No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACCTAGC2672.3225469728601253No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT2642.2964509394572024No Hit
ATCGGTTACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCTAGCT2632.287752261656228No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT2191.9050104384133613No Hit
ATCGGTTACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCTAG2011.7484342379958244No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT1871.6266527487821851No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA1811.5744606819763398No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT1701.4787752261656228No Hit
ATCGGTTACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCTA1251.0873347251217813No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC1070.9307585247042449No Hit
ATCGGTTACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCTA1060.9220598469032707No Hit
ATCGGTTACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC960.8350730688935281No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT910.7915796798886569No Hit
ATCGGTTACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT870.75678496868476No Hit
ATCGGTTACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTACCT840.7306889352818371No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTACC760.6610995128740431No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT740.6437021572720947No Hit
ATCGGTTACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACC720.6263048016701461No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC600.5219206680584552No Hit
ATCGGTTACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT560.4871259568545582No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT490.42623521224773836No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT490.42623521224773836No Hit
ATCGGTTACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT460.4001391788448156No Hit
ATCGGTTACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC450.3914405010438413No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA450.3914405010438413No Hit
ATCGGTTACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAC430.3740431454418929No Hit
ATCGGTTACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTACCT420.36534446764091855No Hit
ATCGGTTACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACCTA400.3479471120389701No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT400.3479471120389701No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCTAGAGGAGCCTGTTCTACC390.33924843423799583No Hit
ATCGGTTACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT390.33924843423799583No Hit
ATCGGTTACTGGGATTAGATACCCCTACCCTAGAGGAGCCTGTTCTACCT370.32185107863604734No Hit
ATCGGTTACTGGGATTAGATACCCCTGTGCTAGAGGAGCCTGTTCTACCT350.3044537230340988No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAG340.29575504523312457No Hit
ATCGGTTACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT340.29575504523312457No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTG310.2696590118302018No Hit
ATCGGTTACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTACCT310.2696590118302018No Hit
ATCGGTTACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACCTAG290.2522616562282533No Hit
ATCGGTTACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTACCTAG280.2435629784272791No Hit
ATCGGTTACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTA280.2435629784272791No Hit
ATCGGTTACTGGGATTAGATACCCCCCACTAGAGGAGCCTGTTCTACCTA270.23486430062630478No Hit
ATCGGTTACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACCTAG270.23486430062630478No Hit
ATCGGTTACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT260.22616562282533054No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT240.20876826722338201No Hit
ATCGGTTACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAC240.20876826722338201No Hit
ATCGGTTACTGGGATTAGATACCCCAGTGTCAATCACTGTCTCTTATACA230.2000695894224078No Hit
ATCGGTTACTGGGATTAGATACCCCATGTGTATAGAGGAGCCTGTTCTAC230.2000695894224078No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT230.2000695894224078No Hit
ATCGGTTACTGGGATTAGATACCCCGGGCAGTCCCTAGAGGAGCCTGTTC220.19137091162143355No Hit
ATCGGTTACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA220.19137091162143355No Hit
ATCGGTTACTGGGATTAGATACCCCACCAGACCTAGAGGAGCCTGTTCTA210.18267223382045927No Hit
ATCGGTTACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTT210.18267223382045927No Hit
ATCGGTTACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTACCT210.18267223382045927No Hit
ATCGGTTACTGGGATTAGATACCCCCCGAGACCTAGAGGAGCCTGTTCTA200.17397355601948505No Hit
ATCGGTTACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCC200.17397355601948505No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG200.17397355601948505No Hit
ATCGGTTACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA200.17397355601948505No Hit
ATCGGTTACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATA200.17397355601948505No Hit
ATCGGTTACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTACCTAG180.15657620041753653No Hit
ATCGGTTACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC180.15657620041753653No Hit
ATCGGTTACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTAC170.14787752261656228No Hit
ATCGGTTACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCC170.14787752261656228No Hit
ATCGGTTACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT160.13917884481558804No Hit
ATCGGTTACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACCTAG160.13917884481558804No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTACCTA150.1304801670146138No Hit
ATCGGTTACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAC150.1304801670146138No Hit
ATCGGTTACTGGGATTAGATACCCCGAGCCCACGAGATCTAGAGGAGCCT150.1304801670146138No Hit
ATCGGTTACTGGGATTAGATACCCCGCACCTAGAGGAGCCTGTTCTACCT150.1304801670146138No Hit
ATCGGTTACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTACCT150.1304801670146138No Hit
ATCGGTTACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACCTA140.12178148921363954No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGAT140.12178148921363954No Hit
ATCGGTTACTGGGATTAGATACCCCTGCGCTGCCCTAGAGGAGCCTGTTC140.12178148921363954No Hit
ATCGGTTACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTAC130.11308281141266527No Hit
ATCGGTTACTGGGATTAGATACCCCCGAGCCCACGAGACCTAGAGGAGCC130.11308281141266527No Hit
ATCGGTTACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACCTAG120.10438413361169101No Hit
ATCGGTACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA120.10438413361169101No Hit
ATCGGTTACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC120.10438413361169101No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTGG11150.0295.06
CTGGGGA108.3560945E-4295.09
TCGGTTA11350.0295.02
GGTTACT11250.0295.04
ATCGGTT11350.0295.01
CTGGATT108.3560945E-4295.09
TACTGGA108.3560945E-4295.07
CGGTTAC11250.0295.03
ACTGGGG108.3560945E-4295.08
GTTACTG11250.0293.688875
TACTGGG11050.0293.665167
ACTGGGA11000.0293.65918
CTGGGAT10950.0293.652989
CGGTTAT150.0028131178196.666663
ACTGGAT150.0028131178196.666668
GCCCCAA452.5465852E-1159.000004120-124
TGCCTCA900.059.000004115-119
CCCCTGT452.5465852E-1159.00000420-24
GACCCAC900.059.000004125-129
GGTGCCC452.5465852E-1159.000004115-119
Produced by FastQC (version 0.11.5)