FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-1-5-8-2-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-1-5-8-2-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11036
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA366333.19137368611816No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA8487.683943457774555No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAA6936.279449075752084No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAAGTCTCCTG4343.932584269662921No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAAGTCTC3312.999275099673795No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA3162.86335628851033No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA3052.763682493657122No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT1981.7941283073577383No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAA1901.7216382747372236No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA1661.5041681768756796No Hit
CAACACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1361.2323305545487495No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAAGTCTCCT1080.9786154403769481No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAAGTCT1020.9242479159115622No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAAGTCTC1010.9151866618339978No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA950.8608191373686118No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA830.7520840884378398No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA830.7520840884378398No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAA750.6795940558173251No Hit
CAACACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC710.6433490395070678No Hit
CAACACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA700.6342877854295035No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAA650.5889815150416817No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA650.5889815150416817No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA630.5708590068865531No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG620.5617977528089888No Hit
CAACACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAAGTCT580.5255527364987314No Hit
CAACACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAAGTCTCC580.5255527364987314No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA550.49836897426603843No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC540.48930772018847407No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA530.4802464661109097No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA490.44400144980065237No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA410.3715114171801377No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA400.3624501631025734No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA400.3624501631025734No Hit
CAACACACTGGGATTAGATACCCCGAGCCTAGAGGAGCCTGTTCTAAGTC380.3443276549474447No Hit
CAACACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAAGTCT330.299021384559623No Hit
CAACACACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAA300.27183762232693004No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT300.27183762232693004No Hit
CAACACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAAG280.25371511417180137No Hit
CAACACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA250.22653135193910837No Hit
CAACACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAAG250.22653135193910837No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA240.21747009786154403No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA240.21747009786154403No Hit
CAACACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAAG240.21747009786154403No Hit
CAACACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAAGT230.2084088437839797No Hit
CAACACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAAGTCT230.2084088437839797No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAAG230.2084088437839797No Hit
CAACACACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAAGTC220.19934758970641536No Hit
CAACACACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAAGT220.19934758970641536No Hit
CAACACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAAGTCTCC220.19934758970641536No Hit
CAACACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAAG210.19028633562885103No Hit
CAACACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA210.19028633562885103No Hit
CAACACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAAGTCTC200.1812250815512867No Hit
CAACACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAAG190.17216382747372236No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG180.16310257339615802No Hit
CAACACACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTT170.1540413193185937No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT160.14498006524102935No Hit
CAACACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAAGTC160.14498006524102935No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC160.14498006524102935No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG150.13591881116346502No Hit
CAACACACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTAA150.13591881116346502No Hit
CAACACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT140.12685755708590069No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA140.12685755708590069No Hit
CAACACACTGGGATTAGATACCCCTCTTATACACATCTACGCTAGAGGAG140.12685755708590069No Hit
CAACACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA140.12685755708590069No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATG140.12685755708590069No Hit
CAACACACTGGGATTAGATACCCTATAAGAGGAGCCTGTTCTAAGTCTCC130.11779630300833635No Hit
CAACACACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTTC130.11779630300833635No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACA130.11779630300833635No Hit
CAACACCCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA120.10873504893077202No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGGG10600.0295.000036
ACTGGGA10600.0295.000037
CTGGGAT10750.0295.08
ACACTGG10650.0295.05
CGCTGGG108.351608E-4294.999976
ACGCTGG108.351608E-4294.999975
CACGCTG108.351608E-4294.999974
GCTGGGA108.351608E-4294.999977
ACACTTG108.351608E-4294.999975
TTGGATT108.351608E-4294.999979
TGGGATA108.351608E-4294.999979
CACACTG10700.0293.62154
TGGGATT10700.0293.62159
ACACACT11200.0283.14733
AACACAC12900.0245.833332
CAACACA13650.0234.487181
ACACGCT150.0028116107196.666663
CACACTT150.0028116107196.666664
ACTTGGA150.0028116107196.666667
CTTGGAT200.006647876147.499988
Produced by FastQC (version 0.11.5)