FastQCFastQC Report
Wed 31 Jan 2018
000000000-BKN32_l01n03.333000000bcaa1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BKN32_l01n03.333000000bcaa1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18304411
Sequences flagged as poor quality0
Sequence length251
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATAGACTTAGTA362521819.805160624944445No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC10224855.586003286311699No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG7078363.8670241834058467No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG5269242.8786722500931607No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG3746682.046872745591213No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG3590731.9616747023436045No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGGCCAGAG2393141.3074116397408253No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG1998051.0915674915734792No Hit
CCTGTTTGCTCCTCACGCTTTCGTGCCTCAGCGTCAGTATAGACTTAGTA1578990.8626281391955195No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTATAGACTTAGTA987740.5396185651644295No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTCTTGGTCCAGAT816830.4462476285087786No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACGGCCCAGAG788500.43077048477550034No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA756990.41355605487660874No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA707310.3864150559119329No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG705760.38556826548529755No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATCGACTTAGTA697100.3808371654242248No Hit
CCTGTTTGATACCCACACTTTCGTGCTTCAGTGTCAGTTGTGGCCTGGTA650200.35521492606344995No Hit
CCTGTTTGATCCCCACGCTTTCGCACCTCAGCGTCAGTTTTGTGCCAGAT586360.32033808681415643No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAATGTCCAGCA543500.2969229657266765No Hit
CCTGTTCGCTACCCACGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG529900.2894930626284561No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTGGTA505940.27640332158188535No Hit
CCTGTTTGATACCCACGCTTTCGCACCTCAGCGTCAGTATAGTGCCAGAT483350.26406203400917955No Hit
CCTGTTTGATACCCACGCTTTCGCACCTCAGCGTCAGTTTAGTGCCAGAT476670.26041264042858303No Hit
CCTGTTCGCTACCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG474020.2589649019572386No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTCAGCGTCAATATTGTTCCAGTA448190.2448535492346626No Hit
CCTGTTCGCTACTCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC441910.24142268221577848No Hit
CCTGTTTGATACCCACGCTTTCGCACCTCAGCGTCAGTATTGTGCCAGAT438260.23942862734015313No Hit
CCTGTTTGCTACCCACGCTTTCGCATCTGAGCGTCAGTCATCGTCCAGAA410550.224290199777529No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATTGACTTAGTA410200.22409898903603073No Hit
CCTGTTTGATCCCCACGCTTTCGCACCTCAGCGTCAGTATTGTGCCAGAT390000.2130633976695563No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAACGTCAGTTACAGTCCAGAA382650.20904797209809153No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATGGTCCAGAA348450.1903639510716843No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGGCTTGGTA341970.18682382077194398No Hit
CCTGTTTGATACCCACGCTTTCGCACCTCAGCGTCAGTTTTGTGCCAGAT340710.18613546210255003No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTAGACTTAGTA336460.18381361738435614No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG329670.18010412899928877No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTAGCCCAGGG328870.179667075875864No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAATAACGGCTTTGTG322890.17640010377826418No Hit
CCCGTTTGCTACCTGTACTTTCGCGCCTCAGCGTCAGTCTTCCCCCAGAG320600.17514903921246086No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC318850.17419298550496926No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATAGACTTCGTA313200.1711062978207821No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGGCTTAGTA311960.17042886547947378No Hit
CCTGTTCGCTACCCATGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAC304050.16610750272161176No Hit
CCTGTTTGCTACCCACGCCTTCGCGCATCAGCGTCAGTTTTGGTCCAGAA303980.1660692605733121No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA289690.15826239915613785No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTATAGACTTAGTA286340.15643223920179677No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTACTCAGCCAGAA277300.15149353890709732No Hit
CCTGTTTGCTCCCCACGCTCTCGTGCCTCAGCGTCAGTATAGACTTAGTA273540.1494393892270011No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC267740.14627075408217177No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTTGGCCAGAT265920.14527645822638052No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAC264030.1442439202222896No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG259240.14162706464578403No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATAGACTCAGTA248930.1359945425176478No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATAACAGCTTGGTA244940.13381474006456695No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC242960.13273303358409075No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAATTACAGACCAGAG240290.1312743687846607No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC233830.127745164813006No Hit
CCTGTTTGCTACTCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG233180.1273900591502234No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATCGAGCCAGTG230880.1261335314203773No Hit
CCTGTTTGATCCCCACGCTTTCGCATCTCAGCGTCAGTACTGCACCAGGT227910.12451097169966299No Hit
CCGGTTCGCGCCCCTAGCTTTCGTTCCTCACCGTCAGATTCGTTCCAGTC227190.12411762388858073No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG216960.118528807072787No Hit
CCCGTTCGCTACCCTAGCTTTCGTGCCTCAGCGTCAGATAAGGCCCAGTA214300.11707560543739977No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA212200.11592834098840983No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG207030.11310388517827752No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTGTGCCAGAT205980.11253025295378256No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCGTATAGACTTAGTA205520.11227894740781334No Hit
CCTGTTTGCTACCCACGCCTTCGCACCTCAGCGTCAGTATTGGTCCAGGA202550.11065638768709901No Hit
CCTGTTTGCTCCCCTAGCTTTCGTGCCTCAGCGTCAGTTGTCACCCAGCA190700.10418253829637021No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACACTTTAGCA188240.10283859994183915No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGGCCAGAG186300.10177874611753418No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA184660.10088278721451349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC11234950.0242.542363
CTGTTCG2822850.0242.278812
CTGTTTG13234950.0241.997182
TGTTTGA2018300.0241.608383
TTTGCTC9753000.0241.603785
TGTTCGC2767750.0241.437183
GTTTGCT11419650.0241.362294
TTGATAC1097250.0241.326226
CCTGTTT13255950.0240.840231
CCTGTTC2832700.0240.412451
CTCCCCA9774500.0239.846959
TTGCTCC9912500.0239.732536
TTTGATC949500.0239.692435
TTTGCTA1843650.0239.305655
GTTTGAT2038950.0239.2154
TGCTCCC9537650.0238.952657
TTGCTAC1882400.0237.693086
GCTCCCC10121300.0237.346778
GTTCGCT3042100.0236.964314
TGATCCC931400.0236.884667
Produced by FastQC (version 0.11.2)