FastQCFastQC Report
Wed 31 Jan 2018
000000000-BKN32_l01n02.332000000bcaa4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BKN32_l01n02.332000000bcaa4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18304411
Sequences flagged as poor quality0
Sequence length12
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTAGTCCTCA7088143.8723671578397143No Hit
ACGACTATGTGC6177253.3747330083442733No Hit
CTATGCTTGATG5857693.2001521381922644No Hit
GTCAACGCGATG5755003.144050906636657No Hit
CAGTGATCCTAG5292022.89111733778268No Hit
CAGCTTACTAGG4593542.509526255720547No Hit
CAAGTGAGAGAG4128062.2552268958558677No Hit
CTGGAGCATGAC4095582.237482539044824No Hit
AGTGTATTTGCC4019432.195880544858832No Hit
CTACATCTAAGC3890412.1253948023785085No Hit
TCCTACTCCGGT3657941.9983926278753246No Hit
GAGTTAGGGAAG3609391.9718689664474864No Hit
GCAAGCCGATTG3588961.9607077223080274No Hit
ACCGATAATTCC3585441.9587846885649585No Hit
GGCCATTAGTCA3576761.9540426621758002No Hit
AGCTCTCAGAGG3559571.9446514831862112No Hit
CCGCTACGTCTT3467911.894576121569823No Hit
GAGAGCTCTACG3425231.8712593374351134No Hit
CACTGGTATATC3413081.8646215931231003No Hit
GGAAAGTCGAAG3195721.7458742594886008No Hit
ATTTGGGTCATC3175661.7349151524187256No Hit
TGCAGAGCTCAG3133281.7117622632053007No Hit
TACTAGGGCTTG3084721.6852331386134196No Hit
GATCCGACACTA3076091.6805184280444752No Hit
GTCTTCGTCGCT3056851.6700073004261105No Hit
GGTGTAAGGTAA3033231.6571033069569954No Hit
CGCGTAACTGTA3013051.6460786419186062No Hit
GGTTCTTATGAC2994211.635786040861954No Hit
GCCGTTCTTCCA2991691.634409323523166No Hit
TAACTTGCGGAC2949501.6113602344265545No Hit
ACATGTCACGTG2848891.5563953409918516No Hit
ACACATGTCTAC2817311.5391426689446603No Hit
ACTGCATCGAGG2755181.5052000307466873No Hit
ACTTCAACTGTG2625271.4342280666665537No Hit
GTAGTGTCTAGC2559711.398411563201897No Hit
GTTGCTTGCAAT2548451.3922600404896939No Hit
CGATCGAGTGTT2529041.3816560390826014No Hit
TGGCAGTTTGTT2503251.3675665390161966No Hit
CGTTCAATCCAA2458911.343342869650381No Hit
AACAGCCGGTCA2394951.3084004724325737No Hit
ACTCGTCGGTAA2337911.2772385847323904No Hit
TGAATAGTCCGC1921291.0496322443808763No Hit
TATTGCTCCTCC1835371.0026927389250602No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC2250.06.02
GGCAGTA806.539676E-56.02
AACCGGT600.00339665476.04
CGGAACT1101.815497E-76.03
AACCGCT650.00126200886.04
TACCCCG704.697551E-46.04
CAAACCG1701.8189894E-126.02
ACTGCAC650.00126200886.01
CGTATGA1054.8311813E-76.05
CCCTCAG1351.375156E-96.03
GTCGAAA2000.06.06
GGTCGAA751.7514337E-46.05
GAATGGC550.009160916.02
CAACGCA1303.6470738E-96.03
TCACAGG1001.2867004E-66.06
CAGCCAG806.539676E-56.03
CGTGAAG550.009160916.06
TAGCGGA1101.815497E-76.05
TAAACTG704.697551E-46.04
ACACAGG751.7514337E-46.01
Produced by FastQC (version 0.11.2)