FastQCFastQC Report
Wed 31 Jan 2018
000000000-BKN32_l01n01.331000000bcaa7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BKN32_l01n01.331000000bcaa7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18304411
Sequences flagged as poor quality0
Sequence length251
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGACACAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA385162321.04204828005665No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA198721710.85649245965904No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA8669814.736459424998706No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA6530053.567473435774579No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA5766183.1501587240365176No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGCGCGCGTA2196611.2000440768074974No Hit
TACGGAAGACACAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1729930.9450891372576807No Hit
TACGTAGGTCCCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1618230.8840655948995026No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1497290.817994088965769No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA1481260.8092366370051458No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATCACTGGGCGTAAAGCGCGCGTA1343560.7340088681356641No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1136510.6208940566293011No Hit
AACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA1065140.5819034548557722No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGAGCGCA1063870.5812096330223354No Hit
TACGGAGGACACAAGCGTTATCCGGATTTATTGGGCTTAAAGGGTGCGTA961430.5252449805677987No Hit
TACGTAAGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA889310.48584464149105916No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA876090.47862233862646547No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA835410.45639818730031795No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATGACTGGGCGTAAAGGGCGCGTA827370.4520058034098994No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA635600.34723870656094863No Hit
TACGTATGTTCCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA625060.3414805316598278No Hit
GACGAACTGTCCAAACGTTATTCGGAATCACTGGGCTTAAAGGGTGCGTA546220.2984089463463206No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA464670.25385684357721205No Hit
CACGGGGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGCGTGTA458190.25031671327747174No Hit
TACGTATGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA453700.24786375262225044No Hit
TACGGAGGACACAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA450230.24596803469939565No Hit
TACGGGGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGAGCATGTA442640.24182149319090354No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA432930.23651676090533585No Hit
TACGAGATGTGCGAGCGTTGTTCGGAATCACTGGGCATAAAGGGTGCGTA407450.2225966189242582No Hit
TACGTAAGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA396200.21645055937609795No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA395980.21633036976715614No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA369630.20193493251435407No Hit
TACGGAGGATGCGAGCGTTATTCGGATTTATTGGGTTTAAAGGGTGCGTA365710.19979337220957286No Hit
CACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA363540.19860786561228327No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATGATTGGGCGTAAAGGGCGCGTA354120.1934615650839571No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA350530.1915002891925886No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA337490.18437632328076548No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATGACTGGGCGTAAAGGGCGCGTA328430.17942669665798042No Hit
TACGGAGGGTGCAAGCGTTATTCGGAATTATTGGGCGTAAAGGGCGCGTA327150.17872741166050085No Hit
TACGGAGGGTCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA323020.17647112491082068No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGGGTGCGTA318340.173914364138786No Hit
TACGGAGGATGCGAGCGTTATCCGGATTCATTGGGTTTAAAGGGTGCGTA314080.1715870562565493No Hit
TACGGAGGACACACGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA312310.17062007622097208No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA309290.16897020068004373No Hit
TACGGAGGACACAAGCGTTATCCGGATTTATTGGGTTTAAAGGGCGCGTA299820.1637965843315035No Hit
TACGTAGGGTCCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA293090.16011987493069293No Hit
GACGAACCGAGCAAACGTTATTCGGAATCACTGGGCTTAAAGGGTGCGTA290840.15889066302106086No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA261700.14297100300031507No Hit
TACGGAGGATGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGCA259400.14171447527046896No Hit
TACGGAAGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA258290.14110806406171714No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATGATTGGGCGTAAAGGGCGCGTA257560.14070925308659207No Hit
TACGGAGGACACAAGCGTTATCCGGCTTTATTGGGTTTAAAGGGTGCGTA254520.13904845121757808No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGCA254080.1388080719996945No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGCGCATGTA249420.13626223755574546No Hit
TACGGGGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA237080.12952069312691897No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA230630.12599695231930708No Hit
TACGTAAGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA224640.1227245170576644No Hit
TACGGAGGGTGCAAGCGTTGTTCGGAATTACTGGGCGTAAAGGGCGCGCA216810.11844685961214486No Hit
TACGGGGGGTGCAAGCGTTATTCGGAATCATTGGGCGTAAAGAGCGCGTA214350.1171029212576138No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA210580.11504330841347475No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGTGCA209860.1146499606023925No Hit
TACGGGGGGTCCAAGCGTTATTCGGAATCATTGGGTGTAAAGCGCATGTA207790.11351908564553102No Hit
TACGTAGGACACAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA200650.10961838651896529No Hit
TACGTAGGGAGCAAGCGTTGTCCGGAATGACTGGGCGTAAAGGGCGCGTA199020.1087278907799874No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA198910.1086677959755165No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATGACTGGGCGTAAAGGGCGCGTA195260.10667374109989117No Hit
TACGGAGGACACAAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA195050.10655901465499217No Hit
TACGGAGGACACAAGCGTTATCCGGATTTATTGGGCTTAAAGGGTGCGCA193960.10596352977432599No Hit
TACGTAGGTGGCGAGCGTTACTCGGAATTACTAGGCGTAAAGCGTATGTA186910.10211199912414555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAG8207300.0261.259121
TACGTAG5917900.0260.332861
AACGTAG183250.0258.045261
TACGTAT197100.0257.144381
TACAGAG154800.0250.238241
ACGGAGG8658500.0248.590712
ACGTATG204950.0247.301882
ACGTAGG6590450.0244.362262
GACACAA5463950.0243.710958
ACACAAG5472500.0243.664679
CGGAGGA6990200.0243.479893
GGACACA5324800.0243.21337
TAGGTCC417800.0242.729145
AGGATCC307300.0242.722556
ACGAGAT56550.0242.603232
GTCCCAA239200.0242.287128
TACGTAA295650.0242.17481
AGGTCCC426900.0242.128896
AGGACAC5351350.0242.084186
GGTCCCA226700.0241.91957
Produced by FastQC (version 0.11.2)