FastQCFastQC Report
Wed 7 Mar 2018
000000000-BKDWB_l01n02.332000000c749c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BKDWB_l01n02.332000000c749c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18363582
Sequences flagged as poor quality0
Sequence length12
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCGACACTA4251272.3150548732812584No Hit
TACTAGGGCTTG3987202.1712539525240775No Hit
CAAGTGAGAGAG3942842.1470974453676845No Hit
CGCGTAACTGTA3635421.9796900190823337No Hit
AACAGCCGGTCA3592301.9562087614496997No Hit
ACACATGTCTAC3591991.95603994906876No Hit
GTGCCATAACCA3548641.9324334435405905No Hit
CAATTGTGCACG3411751.857889163454058No Hit
ACATGTCACGTG3408211.8559614349749411No Hit
CCGCTACGTCTT3405711.854600044806073No Hit
GGAAAGTCGAAG3369741.8350123630564013No Hit
GGTTCTTATGAC3367971.834048498816843No Hit
GAGTTAGGGAAG3345521.8218232151004092No Hit
CGATCGAGTGTT3324331.8102840720290847No Hit
GTTGCTTGCAAT3286941.7899231206634958No Hit
CTACATCTAAGC3270731.7810958668085561No Hit
CTGGAGCATGAC3219831.7533779629704052No Hit
CACTGGTATATC3217201.751945780512756No Hit
ACTTCAACTGTG3216621.7516299379935787No Hit
TGCAGAGCTCAG3187861.7359685054909222No Hit
GCAAGCCGATTG3163341.7226159907146654No Hit
GATGTGAGCGCT3099721.6879713336973146No Hit
ACTCGTCGGTAA3076591.6753757518549486No Hit
GGTGTAAGGTAA2984491.6252221380338543No Hit
GTCAACGCGATG2980501.6230493593243411No Hit
AGTGTATTTGCC2970751.6177399376657562No Hit
CGTTCAATCCAA2920511.5903814408321861No Hit
TGAATAGTCCGC2919181.5896571812623483No Hit
ACCGATAATTCC2911591.5855240007096656No Hit
TATTGCTCCTCC2886591.5719100990209862No Hit
ACTGCATCGAGG2877381.5668947376388767No Hit
GTCTTCGTCGCT2743751.4941257103325485No Hit
CAGTGATCCTAG2729701.4864746975835108No Hit
TGGCAGTTTGTT2648431.4422186259739522No Hit
TAACTTGCGGAC2603621.4178170685871634No Hit
AGCTCTCAGAGG2596031.4136838880344804No Hit
CTATGCTTGATG2580381.4051615855773671No Hit
AGGTAGTCCTCA2579081.4044536626895558No Hit
GTAGTGTCTAGC2569291.3991224587882691No Hit
GCCGTTCTTCCA2548641.38787737599342No Hit
GAGAGCTCTACG2515481.3698198967935558No Hit
ACGACTATGTGC2447941.33304057999142No Hit
TCCTACTCCGGT2405651.31001130389485No Hit
ATTTGGGTCATC2392501.3028503916066048No Hit
GGCCATTAGTCA2192991.194206010570269No Hit
ATCAGAACCTCG2086501.1362162349371707No Hit
GAACTTAGGCCG1939571.056204611932465No Hit
CAGCTTACTAGG1810180.9857445023525366No Hit
TCAAGGCATCGG929830.5063445682873854No Hit
AGATTACCGGCG427740.2329284123326266No Hit
GTTACTGGGTGG369190.2010446545777398No Hit
TAGTGCTGCGTA280610.15280787811441143No Hit
CGCAAGGATTGC211670.11526618281770953No Hit
TCATCGCGATAT211640.1152498461356831No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAC550.0091609136.00000054
CGCGGTG1101.8155151E-76.00000056
ACCAGGG600.0033966556.00000052
TCCCCGC550.0091609136.00000052
GCCAACG953.4300592E-66.00000051
AGGTAGC1451.9645086E-106.01
TGGAGCC3050.06.02
CCGGGCA704.697554E-46.06
GCAGTTG1351.375156E-96.03
CGCGCTG3000.06.06
GCCGGTA1850.06.05
AGGACCT704.697554E-46.04
GATTTGG751.7514346E-46.01
CCAGCCG4100.06.02
AGCGGGT704.697554E-46.04
GTAGCAC650.00126200986.03
GCCGAAG1507.4578566E-116.06
AGTTGCG650.00126200986.03
AACGCGG1303.6470738E-96.04
AGCGCAG1601.0913936E-116.06
Produced by FastQC (version 0.11.2)