FastQCFastQC Report
Mon 26 Feb 2018
000000000-BJV33_l01n02.332000000c5cde.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BJV33_l01n02.332000000c5cde.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18691342
Sequences flagged as poor quality0
Sequence length12
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTAGGGCTTG6305793.3736421921978637No Hit
TGGCAGTTTGTT5646423.0208745846071405No Hit
CGCGTAACTGTA5584152.98755969475065No Hit
CACTGGTATATC5576712.9835792421967344No Hit
GATCCGACACTA5291262.830861475864066No Hit
ACCGATAATTCC5277212.8233446266191056No Hit
TGCAGAGCTCAG4808682.5726777670645586No Hit
ACGAGTGCTATC4781912.5583556279693562No Hit
ACACATGTCTAC4561062.4401993179515946No Hit
ACGTCTGTAGCA3980792.129750769099404No Hit
GAACATGATGAG3732681.9970101665252287No Hit
ACATGTCACGTG3018361.6148439207842862No Hit
GAGTTAGGGAAG3012881.6119120820752195No Hit
AGTGTATTTGCC2994481.6020679521031718No Hit
TTGTATGTGCGT2957571.5823208413820689No Hit
CGTTCAATCCAA2951531.5790893987173311No Hit
TCCACAGGAGTT2899601.5513064818994806No Hit
CGATCGAGTGTT2890261.5463095159245388No Hit
CAAGTGAGAGAG2866861.5337903506339994No Hit
ACAGACCACTCA2760831.4770635516700725No Hit
GTCAACGCGATG2697861.4433741568689933No Hit
ACGACTATGTGC2652541.419127636742188No Hit
GTTGCTTGCAAT2648591.4170143588405797No Hit
GTAGTGTCTAGC2500861.3379777653204357No Hit
TAGTCAGGCCAT2499361.337175254724888No Hit
CAGTGATCCTAG2482641.3282299366198533No Hit
TCCTACTCCGGT2465781.3192097175259005No Hit
AGATCGGCTCGA2458531.315330916314088No Hit
AACAGCCGGTCA2452761.3122439255565492No Hit
TGAATAGTCCGC2444761.307963869046963No Hit
AGCTCTCAGAGG2440151.305497486483314No Hit
TCGCAAACTTTG2437931.304309770801904No Hit
GGCCATTAGTCA2356251.2606103938390298No Hit
TATTGCTCCTCC2352681.258700418621627No Hit
CTACGACCATTA2350771.2576785551299634No Hit
CAGCTTACTAGG2319861.2411414867910502No Hit
ATTTGGGTCATC2295441.2280766142955386No Hit
GGTTCTTATGAC2226361.1911183263352627No Hit
GCCGTTCTTCCA2140251.1450488680802051No Hit
TCGACGGTGCAA2065461.1050356897862121No Hit
CTATGCTTGATG1931541.0333875438157412No Hit
ACTCGTCGGTAA1907601.0205794747108046No Hit
GTCTTCGTCGCT1863220.9968358612238758No Hit
CCGCTACGTCTT1813860.9704279125597295No Hit
CTGGAGCATGAC1677120.8972710466696291No Hit
TAACTTGCGGAC1672100.894585311209864No Hit
CTACATCTAAGC1606410.8594406971955251No Hit
GGAAAGTCGAAG1546180.8272172217489788No Hit
AGGTAGTCCTCA1488300.7962510129021234No Hit
GGTGCGTGTATG1350270.7224039878998523No Hit
AAGTTCGCGCTA937030.5013176688971824No Hit
ACTTCAACTGTG841940.45044384721011466No Hit
GGTGTAAGGTAA809310.43298656672164043No Hit
ACTGCATCGAGG783420.4191352338424924No Hit
TCAAGGCATCGG311670.16674565154283733No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGTG1601.0913936E-116.00000053
CATGCAA806.53971E-56.00000055
ACCGCGC806.53971E-56.00000054
TAGCGGA550.0091609336.00000055
TGAACTG550.0091609336.00000054
GCCGGCC1101.8155151E-76.00000055
GGCCCTA806.53971E-56.00000056
TGGCAGC1601.0913936E-116.00000051
GTAGCTG2250.06.00000054
CTCGGAT550.0091609336.00000053
CGTACTG806.53971E-56.00000053
GCTACCT550.0091609336.00000053
GCAGCAT806.53971E-56.00000053
GCAGAAC806.53971E-56.00000052
TCGCCAG751.7514417E-46.01
AACCGGT953.4300847E-66.04
AGCCGGA1001.2867113E-66.04
GCCTTCG1653.6379788E-126.01
ATGATTC3600.06.05
CATGCTG7950.06.04
Produced by FastQC (version 0.11.2)