FastQCFastQC Report
Mon 25 Jun 2018
000000000-BJT65_l01n03.333000000ea318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BJT65_l01n03.333000000ea318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14542684
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAAAA9692026.664533176956882No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG9123496.273594337881508No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG6641124.566639830721757No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG6184424.252598763749525No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAA5852854.024600960868022No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG5428583.7328597664640175No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG5097323.505075129185232No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG3330632.2902443592943365No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA2439341.6773657462405154No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTATATTCCCAAGG2027601.3942405679721845No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA1601101.1009659564905625No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTGCAGACCAGAA1596411.0977409672107294No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT1401460.9636873083400561No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA1327650.9129332659638344No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAG1268110.8719917176224142No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA1258340.8652735629819089No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG1181420.8123809882687405No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG1178690.8105037557028676No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA1125630.7740180560892336No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG1124450.7732066515369516No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG1076390.7401591068058688No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATTAGCCCAGGT1053050.7241097998141196No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTGCGGTTTGGTA1007800.6929944981270307No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG980250.6740502647241733No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA966300.6644578126018553No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAACGTCAGTTACAGTCCAGAA870500.5985827650521733No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATAATTTGTTAGTA749840.5156132114264464No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA748740.5148568173522852No Hit
CCTGTTTGCTCCCCATGCTTTCGCGCCTCAGCGTCAGTTATAGACCAGAA697140.4793750589643563No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG643600.4425592964819974No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA624870.4296799682919604No Hit
CCCATTTGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA590750.40621799937343067No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA578270.3976363647865827No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA563220.38728751859010346No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAGTGTCAGTGCCGGTCCAGTG558870.3842963238422839No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA529810.364313767664896No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGTGTCAGTATGATGCCAGGA499680.3435954463426421No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAGTTGTAACTTGGTA496370.34131938781039317No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG489650.3366985076482443No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG484770.33334286848287425No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAGTGTCAGTGCCGGCCCGGTG476930.32795184162703395No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT453330.311723750581392No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACAGACCAGAG399320.27458480154007336No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCAGTCCAGAA378380.2601858088919487No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG359990.2475402752339252No Hit
CCTGTTCGCTACCCATGCTTTCGAGTCTCAGCGTCAGTTGCAGACCAGGT336930.2316835049155988No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTATTGGCCCAGGG330140.2270144905850942No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAA327830.22542606302935553No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAG310660.2136194391626745No Hit
CCGTTTTGCTCCCCATACTTTCGTACCTCAGCGTCAGTGTTTAGTTAGAA298200.2050515571953568No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATTGGCCAGTA275430.18939419986021838No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGA240550.16540963139954082No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG236040.1623084156954796No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGTGTCAGTTGCAGTCCAGTG234700.16138699018695585No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAAAG232200.15966791274568023No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATGAGCGTCAGTTGTATTATAGTA209500.14405868957889753No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGTTGGTCCAGGT199200.13697609052084195No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA190810.13120686662792094No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTTTAGTCCAGAA190380.13091118530802154No Hit
CCTGTTTGCTCCCCACGCTCTCGAGCCTCAGCGTCAGTTACAGTCCAGAG187560.12897206595426264No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG185950.12786498008208114No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG179010.12309282110509999No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA176840.12160066188607276No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG170330.11712418422899101No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATACCTTAGTA160860.11061231888143894No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTAAGTTCCAGCA157030.10797869224140468No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTATCTGTCCAGAA150890.10375663804563175No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTG149510.10280770729804758No Hit
CCTGTTTGCTCCCCACGCTCTCGTGCATGAGCGTCAGTATTGGCCCAGGG147960.10174187928445672No Hit
CCTGTTTGCTACCCACGCTTTCGCACATGAGCGTCAGTATTGCCCCAGAA147330.10130867176925526No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTCATCGTCCAGCA146870.10099236152006054No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCACAGTCCAGAG145900.10032535947284558No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTATTATCCCAGGG145860.10029785423378518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC9602300.0244.795873
TTTGCTC8817100.0244.737435
CTGTTCG836750.0244.165532
TGTTTGA1423500.0244.139453
TTGCTCC8925050.0244.128436
GTTTGCT9664300.0243.693154
TTGATAC814100.0243.585546
CTGTTTG11053900.0243.357642
TTTGATC627750.0243.107125
CTCCCCA9473000.0242.705959
TGCTCCC8970250.0242.464057
CCTGTTC833250.0242.089111
GCTCCCC9934550.0241.191048
ATTCGCT153400.0241.087024
CCTGTTT11016150.0240.854451
TGATCCC644950.0238.67517
CATACGT186950.0237.46455245
GTTTGAT1473550.0236.15474
TGTTCGC848100.0235.78473
CCATTCG150050.0235.366552
Produced by FastQC (version 0.11.2)