FastQCFastQC Report
Mon 25 Jun 2018
000000000-BJT65_l01n02.332000000ea31b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BJT65_l01n02.332000000ea31b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14542684
Sequences flagged as poor quality0
Sequence length12
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTCAGCGCA1581811.08770155495368No Hit
CCTGAACTAGTT1473271.0130660887632572No Hit
CAAGCATGCCTA1286930.884932932600337No Hit
GAATAGAGCCAA1209540.8317171713282088No Hit
ATGTGCACGACT1194000.8210313859532395No Hit
GGAAACCACCAC1184280.8143476128615598No Hit
TGTTTGAGCTGT1163140.7998110940181331No Hit
GACTTTCCCTCG1150650.79122258312152No Hit
TTGCGCATACTA1149520.7904455601180634No Hit
TGGAGCACGTTG1140780.7844356653833638No Hit
ATCGATCTGTGG1121600.7712469032538973No Hit
CGCCAAATAACC1114150.7661240524788959No Hit
ATCGGCGTTACA1093400.7518557097163082No Hit
GCTGATGAGCTG1084750.7459077017694945No Hit
CTTGTGTCGATA1078480.7415962555467752No Hit
CGGAGCTATGGT1075970.7398703017957345No Hit
GACGGAACCCAT1061690.7300509314511682No Hit
GATAGAAAGCCA1026840.706086991919786No Hit
TATCGTTGACCA1022250.7029307657376038No Hit
ATTCCTGTGAGT1010430.6948029675952527No Hit
ACACGTAAGCCT994520.6838627587589746No Hit
TCCAAAGTGTTC978550.6728812920641059No Hit
TCACGGGAGTTG971080.6677446886695743No Hit
GTCTAATTCCGA966060.6642927811674928No Hit
GCACGACAACAC948160.6519841866879593No Hit
GCTAATTACGCT947870.6517847737047714No Hit
ACACCTGGTGAT939960.6463456126805753No Hit
AGATGTTCTGCT930850.640081294484567No Hit
GACAGGAGATAG927320.6376539571374857No Hit
TCGAGGACTGCA922390.6342639364232903No Hit
ATGGATACGCTC915780.6297186956685574No Hit
CACATGCCTAAG903330.6211576900110048No Hit
AAGAGATGTCGA898300.6176989061991582No Hit
ACACTAGATCCG880450.6054246932684503No Hit
GAATCTTCGAGC876210.6025091379280468No Hit
TAGCGGATCACG866230.5956465807824746No Hit
AGCCTAAGCACG862700.5932192434353933No Hit
CGCAGCGGTATA860380.5916239395698896No Hit
ATACCTTCGGTA860300.5915689290917687No Hit
GAACCAAAGGAT854040.5872643591788146No Hit
CGGACTACAACT847950.5830766865318672No Hit
CAGTGCATATGC827510.5690215093719977No Hit
CGGGTTTGACGA823970.5665872957151513No Hit
AGCTGGAAGTCC816090.5611687636202506No Hit
GACTTGGTATTC810570.557373040629914No Hit
AATCAGTCTCGT803340.5524014686697448No Hit
CATTTGGACGAC798440.5490320768848447No Hit
AGCCGGCACATA796700.5478355989857169No Hit
ATGATGACCCGT780680.5368197507420226No Hit
ACTTATGCGGTT772700.5313324555494708No Hit
TCCTCTGTCGAC770560.5298609252597388No Hit
GCTCAGTGCAGA769220.5289394997512151No Hit
GCACTACCGAAT768480.5284306528285976No Hit
CTAGCGAACATC768100.5281693530575237No Hit
AGTTCCCGAGTA764140.525446334390543No Hit
TGCTATATCTGG759280.5221044478447032No Hit
CGTGACAATGTC752810.5176554754266819No Hit
TAAGGTAAGGTG750040.5157507376217485No Hit
TAGGATTGCTCG747770.5141898153050702No Hit
CCTCGTTCGACT746670.513433421230909No Hit
TGCGGCATCGAA734320.5049411786710074No Hit
GTACATACCGGT726010.4992269652562072No Hit
AGTAGAGGGATG722640.49690964886536765No Hit
ACGATGCGACCA714600.49138109581422523No Hit
CTCTACCTCTAC713880.4908860015111378No Hit
ATTCTGTGAGCG708200.4869802575645596No Hit
ACGCGCAGATAC705770.4853093142916397No Hit
GTGCCGGTGATA698500.48031023709241016No Hit
AGCAAACACCCG694800.47776600247932227No Hit
CAGGAAGGTTAA684670.4708003006872734No Hit
TCCAGGCTTAAT669530.46038956770290823No Hit
TACACGATCTAC663140.45599560576300774No Hit
TGGCACCGATTA658230.45261933766834234No Hit
CACGAGGTCATT655110.4504739290216304No Hit
AACACAAGGAGT653130.4491124196881401No Hit
ATCCCGAATTTG645280.44371451652253463No Hit
CTAGATTTGCCA638350.43894923385531853No Hit
CAACGGGTAGTC629430.4328155655448472No Hit
ACCACATACATC627480.4314746851406522No Hit
GGTAACAGCTCG623180.4285178719416581No Hit
TAAACCGCGTGT621780.4275551885745438No Hit
ATACTTCGCAGG619250.4258154822039728No Hit
TCCGAATTCACA617820.4248321699075632No Hit
GACTGATCATCT611580.42054135261413916No Hit
CCATAGGGTTCA606580.417103197731588No Hit
ATTATACCTCGG601040.4132937221217211No Hit
ACACGAGCCACA600820.4131424433068889No Hit
TATGCACCAGTG598200.411340850148432No Hit
AGTTTGCAACGC597710.411003910969942No Hit
CAGCATGTGTTG597450.4108251269160494No Hit
TTCGCCCTTCAG583660.401342695749973No Hit
ATGAGACTCCAC577130.39685246547336106No Hit
TAGGAACTGGCC576560.3964605158167502No Hit
GGATCGCAGATC575630.3958210190085957No Hit
GAATGATGAGTG572970.3939919206110784No Hit
TGTGCTGTGTAG572420.3936137235739977No Hit
CCTTGGCTATCC567990.39056751834805736No Hit
ACCGGCTAGAGT558780.3842344370543979No Hit
AATCCGTACAGC539960.371293222076475No Hit
CGTAACCAACCA528060.36311041345600303No Hit
TTCCTAGGTGAG519840.3574580868290888No Hit
GTCTACACACAT508220.34946781488203965No Hit
TGAGTTCGCTAT506970.34860827616140183No Hit
CAAGTTTACGGC501080.34455812970975647No Hit
TCTACGGAGAGC500140.3439117565918368No Hit
AAGGAGCGCCTT497870.34235083427515856No Hit
TACTTCGCTCGC488390.33583209261784136No Hit
ATGTGGGACCCA486330.3344155728062303No Hit
GACACATTTCTG485370.3337554470687804No Hit
GACTCGAATCGT483920.33275838215284054No Hit
AAGTGGACTCTC482210.33158253318300807No Hit
CTTCGGCAGAAT482000.33143813067794087No Hit
CATCCAAATGCG479080.32943024822653094No Hit
CTACCGGATCAA467890.3217356575993812No Hit
AGCTGTTGTTTG463530.31873758654179657No Hit
TTATGCAGTCGT454290.3123838763188418No Hit
TACGATGACCAC445580.3063946105134375No Hit
GAGGCTCATCAT437340.3007285312669931No Hit
TAATCGGATTCC436260.299985889812362No Hit
GTGGGATGTTTC431300.29657524016887116No Hit
TCAAGCTCAAGC427650.2940653871046087No Hit
TGACCGGTCAAT427520.2939759950776624No Hit
AGCTTGACAGCT402310.276640818159839No Hit
AGAGCCTACGTT387740.26662203483208463No Hit
ATTAGTTCGCGT380440.2616023287035598No Hit
ATGGACCGAACC378010.25993138543063987No Hit
ACGTGAGAGAAT376460.25886555741704903No Hit
CTTGCTGAAGAC371320.2553311341977863No Hit
AATGTCCGTGAC371050.25514547383412856No Hit
TACCCAGAGATC370360.25467100846033647No Hit
GTAAGCACCTAC366510.252023629200772No Hit
TAAGGCCTATCG364720.25079276975281867No Hit
AAGATGGATCAG363040.24963754971228147No Hit
GAGGAATAGCAG355940.24475536977905865No Hit
GGTCACTGACAG355520.24446656476892437No Hit
GTACCTAATTGC352300.24225239302456134No Hit
CTTACACCAAGT340580.23419335797986118No Hit
GTTGGTCAATCT335350.2305970479727126No Hit
TTCTGGGAACAC335240.23052140856529646No Hit
TACTAATCTGCG316420.21758019358737354No Hit
CCTAATGGAACC314470.21623931318317857No Hit
TGTAATTGTCGC312080.21459587514931905No Hit
CACGGTTGTGAG308410.21207226946552646No Hit
TAACACCACATC307020.2111164624081772No Hit
ACGTGTACCCAA304420.20932862186925053No Hit
TAACGCTTGGGT303310.20856535148532418No Hit
CCTCTCGTGATC291430.2003962954843824No Hit
CGAGATACCCAG290410.19969491188834193No Hit
ACACAAAGGGAG289640.19916543603642903No Hit
GTCCGAAACACT286090.19672434606981767No Hit
CTAACCTCCGCT279080.1919040529244808No Hit
CTATTTGCGACA276340.1900199440488427No Hit
GTATGCGCTGTA276240.18995118095119168No Hit
TGTGAGCACGGT264700.18201591948226337No Hit
CCATGCGATAAC258700.17789013362320188No Hit
CGTCCGAAATAC256920.17666615048501363No Hit
TGGCAAGACTCT255540.1757172197374295No Hit
TTGCAGACAGGC255480.17567596187883888No Hit
ACGGGACATGCT253400.17424568944769755No Hit
CTCCTACTGTCT252370.173537429541892No Hit
TGGACACCGAAC242730.16690866692833317No Hit
GAGGCCATCAGT239010.16435067969571504No Hit
TCGGAGTGTTGT233470.16054120408584824No Hit
TTAGGTGCAGCT232560.15991545989722392No Hit
CCATACATAGCT229560.15785256696769318No Hit
TGCAATGTTGCT224400.1543043911289003No Hit
GCCTGAATTTAC223570.15373365741839679No Hit
GCGTGTTAAACC223060.15338296562037654No Hit
TGGCATACGGCA217510.14956661370074464No Hit
TCAACAGCATCG212810.14633474811114647No Hit
CTGTAGGAGACC211100.14515889914131394No Hit
TTATCACGTGCA197670.1359240151267813No Hit
GTCGTCCTAAAT193260.13289156252037107No Hit
CTCGAGAGTACG189570.13035420421704824No Hit
CCAAGTCTTACA186230.128057516755504No Hit
TACCTCTCAGAA184970.1271911017251011No Hit
AATCTTGCTGCA184420.12681290468802045No Hit
TAATCCACAGCG184020.12653785229741635No Hit
ATGTCGAGAGAA181710.12494942474167768No Hit
TGACCTCCAAGA166060.11418799995929224No Hit
CGATCCGTATTA162600.1118087967805668No Hit
ACTCACGGTATG160270.11020661660529789No Hit
GGAGACAAGGGA151700.10431361913660504No Hit
CTGGAAATCTGC150740.10365349339915521No Hit
CTCCTGAAAGTT147800.10163185832821507No Hit
ATTGGGCTAGGC147130.10117114557395321No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGG806.539226E-56.00000054
GCCGCGG550.0091606016.00000052
TCCAGGC68600.06.00000051
CAGGACG550.0091606016.00000051
GGCGCAC1303.6470738E-96.00000052
AGGTACG751.7513274E-46.03
GTGGGCC704.6973006E-46.03
GGGCCCC600.00339651976.05
ATCGGCG113450.06.01
CGGGTTT84950.05.99646851
AGCCGGC84150.05.9964351
CATTTGG79450.05.9962241
GAGGTCA65400.05.9954134
TGGAGCA122650.05.99510771
TCTACGG54450.05.994491
TCACGGG102000.05.99411771
ATGTGGG49300.05.9939151
TGTGGGA48900.05.9938652
TAATCGG46900.05.9936031
GCCGGCA84600.05.9929082
Produced by FastQC (version 0.11.2)