FastQCFastQC Report
Thu 8 Mar 2018
000000000-BHPHD_l01n02.332000000c903d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BHPHD_l01n02.332000000c903d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18742701
Sequences flagged as poor quality0
Sequence length12
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTGTGCCTTG5240882.796224514278918No Hit
GAAGGAAGCAGG4598152.453301687947751No Hit
TCCAATAGCGTG4014202.141740403370891No Hit
CTTGACTGAGGT3871932.0658335210063905No Hit
GAACATGATGAG3805192.030224992651806No Hit
GATGATCGCCGA3689451.968472953818129No Hit
GCGTTACACACA3651931.9484544943655666No Hit
GGTGCGTGTATG3565851.9025272824871935No Hit
CTATGCTTGATG3553031.8956872864802143No Hit
GACTTATAGGCC3455161.843469625855953No Hit
TAGTCAGGCCAT3417621.823440495582787No Hit
ACGAGTGCTATC3391841.8096858078246032No Hit
GCATGAAATGGA3380271.8035127381053562No Hit
TCCACAGGAGTT3297501.7593515470368974No Hit
GTGTGGCCAAAC3272471.7459970150513524No Hit
GTAGATGCTTCG3202321.7085691117838353No Hit
CTACGACCATTA3193511.7038686153079006No Hit
CGCAAGGATTGC3138771.6746625793155427No Hit
ACTCGTCGGTAA3099831.6538864916001168No Hit
ACAGACCACTCA3064531.6350524932345665No Hit
TCGACGGTGCAA3051621.6281644785348708No Hit
GTCAACGCGATG3023911.6133800565884289No Hit
TTCGCGTGAAAC2994251.5975552296331248No Hit
AGATCGGCTCGA2981531.5907685877291646No Hit
TTGTATGTGCGT2976471.5880688701164256No Hit
AGAGAAATGTCG2967531.5832990133065667No Hit
ACCGATAATTCC2942221.56979508983257No Hit
TCAAGGCATCGG2933031.5648918477651645No Hit
TAGTGCTGCGTA2931901.564288946401055No Hit
GTTACTGGGTGG2897801.5460951972717272No Hit
TGAGTGGAATGA2891531.5427498950124638No Hit
CAAGGTTCTTGT2885641.539607338344671No Hit
AGAAGTCCCTGG2871051.5318229747142635No Hit
CTGGAGCATGAC2835081.5126315038584885No Hit
TCGCAAACTTTG2822801.50607962000781No Hit
TCATCGCGATAT2807801.4980765045550264No Hit
GTGTTTGGTCGA2756661.4707912162713368No Hit
GGAAGTGAAGTT2711231.4465524472700064No Hit
AAGTTCGCGCTA2701371.441291732712377No Hit
ACGTCTGTAGCA2631311.4039118481375763No Hit
TCGTGATGTGAC2551821.361500671648126Illumina PCR Primer Index 1 (100% over 12bp)
TTCGTGCCTGCT2280321.2166442819527452No Hit
TGGGCAACCTGA2277271.2150169818106793No Hit
GCTAAGAGAGTA2271301.2118317418604716No Hit
AGATTACCGGCG2040601.0887438261966618No Hit
CAGCACTAAGCG1909521.018807267959938No Hit
CAGCTTACTAGG1843580.983625572429502No Hit
ACGCAACTGCTA1772210.9455467491051583No Hit
TATATCCACTGG1528470.815501458407729No Hit
AGTGTATTTGCC1340970.7154625152479357No Hit
ACGACTATGTGC1301830.6945797193264727No Hit
TAGTTAACGTCC994610.5306652440328637No Hit
ACTTCAACTGTG381080.20332181578311473No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGGA1054.831236E-76.00000053
ACCCTTG1054.831236E-76.00000056
TCAGGCG650.00126201376.00000054
GCACCGG1601.0913936E-116.00000056
GTCAGGT2050.06.00000053
TGACCGA1303.6470738E-96.00000053
GTAGACG953.4300847E-66.00000051
CGCTAGG1303.6470738E-96.00000051
CAGGCGT4200.06.00000055
TAAGGTT1303.6470738E-96.00000051
CCGGTGC1303.6470738E-96.00000054
GTGACCA806.5397144E-56.00000053
GCAGATC1054.831236E-76.00000051
CCTGCGG806.5397144E-56.00000055
CGTCAGC953.4300847E-66.00000052
GGCCTCT650.00126201376.00000055
GGCCTCG3200.06.00000055
CCTGAGT953.4300847E-66.00000055
CCCATAG806.5397144E-56.00000052
GGTACAC1001.2867131E-66.03
Produced by FastQC (version 0.11.2)