FastQCFastQC Report
Mon 26 Mar 2018
000000000-BHPG9_l01n03.333000000cc320.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BHPG9_l01n03.333000000cc320.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17960422
Sequences flagged as poor quality0
Sequence length251
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG221989212.35991002884008No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAAAA14106057.853963564998639No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG10235325.69881932618287No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG8758854.876750668776046No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG8087184.502778386833004No Hit
CCTGTTTGCTCCCCATGCTTTCGCGCCTCAGCGTCAGTTATAGACCAGAA5056972.8156186976007582No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA3013741.6779895260812911No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG2993901.666943015036061No Hit
CCTGTTTGATCCCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAG2974891.6563586312170169No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTATGGTCCAGAA2934171.6336865581443465No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTATATTCCCAAGG2667221.485054193047357No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG2496261.3898671200487382No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATGTTGGCCCAGGT2384161.3274521055240238No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAA2246401.2507501215728674No Hit
CCTGTTTGCTCCCCACGCTTTCGTTCCTCAGTGTCAATATACAACCAGTT2227481.240215847934976No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATAATTTGTTAGTA2214111.2327717021348386No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2210631.2308341084635985No Hit
CCTATTTGCTCCCCACACTTTCGAGCCTAAGCGTCAGTGATAGTCCAAGT1783320.9929165361482041No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCATGAGCGTCAGTATTGGCCCAGGG1342530.7474935722557076No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTATTATCCCAGGG1342470.7474601654682724No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAG1320090.7349994337549529No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACACTCCCAAGG1263570.7035302399910203No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAATTACAGACCAGAG1157140.6442721668789296No Hit
CCTGTTCGCTCCCCACGCTTTCGTGACTCAGCGTCAGTTTCAGTCCAGTA1028840.5728373197467187No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG950540.5292414621438183No Hit
CCTGTTTGCTCCCCACGCTCTCGTGCATGAGCGTCAGTATTGGCCCAGGG797340.4439427982260105No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG660700.3678644076403105No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA550370.3064348933449337No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATAGACCAGAA545300.3036120198066615No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG488790.2721483938406347No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGACCCAGGG458120.2550719576633556No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGTCCCAGGG406450.22630314588376596No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACAGACCAGAG392350.2184525508365004No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG376860.20982803188031995No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTATTGGCCCAGGG366780.20421569159121095No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAGTTGTAACTTGGTA335390.18673837396470971No Hit
CCTGTTTGCTCCCCACGCTCTCGCGCCTCAGCGTCAGTTACAGACCAAAA333930.18592547547045388No Hit
CCTGTTTGCTCCCCACGCTCTCGCACCTGAGCGTCAGTCTTTGTCCAGGG317150.1765827105844172No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGT312190.17382108282310962No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG278620.15512998525313046No Hit
CCTGTTTGCTCCCCACGCTCTCGAGCCTCAGCGTCAGTTACAGTCCAGAG261660.1456870000047883No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATGGTCCAGAA261560.14563132202572968No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAAAA254700.14181181266230827No Hit
CCTGTTTGCTCCCCACGCTCTCGCACCTGAGCGTCAGTCTTCGTCCAGGG252790.14074836326228862No Hit
CCTGTTCGCTACCCATGCTTTCGAGTCTCAGCGTCAGTTGCAGACCAGGT243460.13555360781611925No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA240310.13379975147577267No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCACAGTCCAGAG237830.13241893759511889No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTGAGCGTCAGTATTGGCCCAGGG234160.13037555576366747No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAG233130.1298020725793637No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATCAGCGTCAGTATTGGCCCAGGG204710.1139783909309035No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGCCCAAAA204120.11364989085445765No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTCTTTGTCCAGGG203070.11306527207434214No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAA196380.10934041527532036No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA193880.10794846579885484No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTCTTCGTCCAGGG189210.10534830417681723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCTC13854750.0244.798395
TGTTTGC13851450.0244.6253
GTTTGCT13870400.0244.420614
CTGTTCG1066100.0244.264592
CTGTTTG14432750.0244.035772
TGTTTGA581600.0243.736223
TTTGATC525950.0242.973655
ATTCGCT438000.0242.902394
CCTGTTT14374950.0242.769091
CTATTTG305950.0242.677722
CCATTCG421350.0242.470632
CCTGTTC1068200.0241.439511
CCTATGT208900.0241.18835245
TGTTCGC1078850.0241.082613
GCTCCCC15605350.0241.059348
CTCCCCA15145100.0241.01169
CCCTCCG4538150.0238.54585245
TTGCTCC14367500.0237.610326
TGCTCCC14409000.0237.124077
CATTCGC431750.0236.71513
Produced by FastQC (version 0.11.2)