FastQCFastQC Report
Mon 26 Mar 2018
000000000-BHPG9_l01n02.332000000cc323.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BHPG9_l01n02.332000000cc323.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17960422
Sequences flagged as poor quality0
Sequence length12
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTTGACGA2589191.441608665987915No Hit
TGGCAAGACTCT2289451.2747194915576037No Hit
ATGTGCACGACT1631430.9083472537560642No Hit
TAGCTCGTAACT1614800.8990880058386157No Hit
GTCTACACACAT1544930.8601858018703569No Hit
CCTAATGGAACC1538820.8567838773498753No Hit
CCTTGGCTATCC1425980.793956845780127No Hit
TCTGTTGCTCTC1413920.7872420815056572No Hit
CGGACTACAACT1399050.7789627660196403No Hit
CTAGCGAACATC1372730.7643083219314112No Hit
CAGTGCATATGC1334010.742749808439913No Hit
TGGCACCGATTA1320210.7350662473298233No Hit
AATGGAGCATGA1317810.7337299758324164No Hit
GCGATATATCGC1316540.7330228654983719No Hit
CAGGAAGGTTAA1297350.7223382613170225No Hit
CTGCTAACGCAA1269880.7070435204696193No Hit
GAATAGAGCCAA1269850.7070268170759016No Hit
TACAGATGGCTC1261440.7023442990370716No Hit
GCTGATGAGCTG1222230.6805129634481862No Hit
GTCTAATTCCGA1212340.6750064113192886No Hit
CCTGAACTAGTT1212340.6750064113192886No Hit
CGATCCGTATTA1184820.659683831482356No Hit
GCACACACGTTA1173380.6533142706780497No Hit
AACCGCGGTCAA1147220.6387489113563145No Hit
GGATGGTGTTGC1127420.6277246715027074No Hit
TTAGGGCTCGTA1124660.6261879592806895No Hit
TCGGAGTGTTGT1122310.6248795267728119No Hit
TCCAAAGTGTTC1104690.6150690668626829No Hit
ACGTGTACCCAA1084390.6037664371137828No Hit
AGCTGTTGTTTG1060070.590225552606726No Hit
GGATCGCAGATC1059630.5899805694988681No Hit
CAAGCATGCCTA1055210.587519602824477No Hit
ACACGTAAGCCT1054740.5872579163229016No Hit
TGAGCCGGAATC1051090.5852256700872619No Hit
TGACCTCCAAGA1041310.5797803637353287No Hit
TCGTCGATAATC1034060.5757437102535787No Hit
CCATGCGATAAC1026900.5717571669529814No Hit
GTCATATCGTAC1026280.571411963482818No Hit
ACATTCAGCGCA1022290.569190412118379No Hit
CACGCCATAATG1020900.5684164882094641No Hit
ATGTACGGCGAC1019370.5675646151298672No Hit
ATACCTTCGGTA1006020.5601316049255413No Hit
ACTGACAGCCAT997460.5553655699181234No Hit
GTCCGAAACACT987790.5499815093431546No Hit
GCCTGAATTTAC974060.542336922818406No Hit
TCCGAATTCACA962320.5358003280769238No Hit
TGTTATCGCACA961010.5350709465512559No Hit
CTACCGGATCAA960080.5345531413460107No Hit
CCTCTCGTGATC952020.5300654962338858No Hit
TAGGAACTGGCC944530.525895215602395No Hit
AATGTCCGTGAC940470.5236346896526151No Hit
CAACACGCACGA931320.5185401545687512No Hit
GAGTGGTAGAGA931210.5184789087917868No Hit
GCACGACAACAC930130.5178775866179536No Hit
GCTTCGGTAGAT926670.5159511285425253No Hit
ACACTAGATCCG918060.511157254545578No Hit
ACACGAGCCACA895610.49865754824691755No Hit
GGCCACGTAGTA894890.4982566667976955No Hit
CTCGAGAGTACG891740.49650281045734895No Hit
GAACTAGTCACC875010.4871878845608416No Hit
CTTACACCAAGT874270.48677586751580787No Hit
GACTGATCATCT871930.4854730028058361No Hit
CTTGTGTCGATA849940.4732294152108452No Hit
AGCTTGACAGCT846570.47135306731656973No Hit
ATGGACCGAACC845980.47102456724012387No Hit
TAGGATTGCTCG837050.46605252371018896No Hit
TCGAGGACTGCA827970.4609969632116662No Hit
CTAGATTTGCCA821820.457572767499561No Hit
GCAGGATAGATA820230.45668748763252887No Hit
ACGGCATGGCAT814920.45373098694451613No Hit
ATGAGACTCCAC809340.45062415571304504No Hit
AGCCTAAGCACG804590.44797945170776055No Hit
AGTTCCCGAGTA803810.4475451634711033No Hit
TAACGCTTGGGT802000.44653739205014226No Hit
GACTCTTGGCAA794450.44233370463121635No Hit
TAAACCGCGTGT794030.44209985711917016No Hit
GAATCTTCGAGC776040.43208338868652413No Hit
GTACCTAATTGC761760.4241325732769531No Hit
CATTTGGACGAC743960.41422189300451845No Hit
CGTCCGAAATAC728080.4053802299300095No Hit
GTGGGATGTTTC722830.4024571360294318No Hit
TTATGCAGTCGT702600.39119348086587274No Hit
TTCTGGGAACAC697260.3882202767841424No Hit
CTCTACCTCTAC689250.3837604706615468No Hit
CCATACATAGCT677440.37718490133472365No Hit
TGCAATGTTGCT675930.3763441638509385No Hit
TCTTCCGCTACT675800.37627178247816223No Hit
AGAACACGTCTC667810.3718231119513784No Hit
TAAAGTCACCCT667690.3717562983765081No Hit
ATGGTTGTTGGC664540.3700024420361615No Hit
GCTCAGTGCAGA620490.34547629226083887No Hit
GTATTACGATCC617580.343856063070233No Hit
TCAAGCTCAAGC615150.3425030881791085No Hit
CGCAAATTCGAC575340.3203376847158714No Hit
TCAACAGCATCG572050.31850587920484275No Hit
CGCACATGTTAT562070.3129492168947924No Hit
CACATGCCTAAG556420.30980341107798026No Hit
CCTAGTACTGAT553330.3080829615250688No Hit
TACTAATCTGCG551000.30678566461300294No Hit
ACTATTGTCACG547810.30500953708103296No Hit
TCTAGCGTAGTG543810.3027824179186881No Hit
CGTGACAATGTC541750.3016354515500805No Hit
AGATAGGACAGG540230.3007891462683895No Hit
ACCACATACATC528510.29426368712271905No Hit
TATCAGGTGTGC524760.2921757629080208No Hit
GTCAATTGACCG509460.2836570321120517No Hit
AGAGCCTACGTT502470.27976514137585407No Hit
CCTCTACCTACG482070.26840683364789536No Hit
TGTGATGGAGAA480770.26768301992013327No Hit
AACACAAGGAGT479230.2668255790426305No Hit
GAAGAAGCGGTA475010.26447596832635667No Hit
CGCCAAATAACC470290.26184796771478974No Hit
AACGAGAACTGA469430.26136913709488563No Hit
AGCAAACACCCG462820.2576888226791108No Hit
TCGCATGAAGTC450570.25086827024442965No Hit
AATCAGTCTCGT449290.2501555921124793No Hit
CCTCGTTCGACT440560.24529490454066166No Hit
CACGGTTGTGAG433300.24125268326100577No Hit
GATAGCTGTCTT432200.24064022549136094No Hit
GGTGTCTATTGT431640.24032842880863267No Hit
GGAGGTTATCCG431480.24023934404213887No Hit
CGGAGCTATGGT429780.23929281839814234No Hit
TGACCGGTCAAT428520.2385912758620037No Hit
TCCTCTGTCGAC424920.23658686861589331No Hit
GTACATACCGGT424830.23653675843474056No Hit
GCACTACCGAAT423460.23577397012163748No Hit
TGGAGCACGTTG418590.2330624525414826No Hit
AATCTTGCTGCA415460.23131973179694776No Hit
GACTCGAATCGT414590.23083533337913772No Hit
TAGCGGATCACG408020.22717729015498633No Hit
GACAGGAGATAG407710.2270046884199046No Hit
GCTAATTACGCT398340.22178766178211182No Hit
CTTGCTGAAGAC398280.22175425499467663No Hit
ATCGCTCGAGGA390600.21747818620297452No Hit
GAGGCCATCAGT387530.21576887224587482No Hit
TACTTCGCTCGC386600.21525106704062968No Hit
TACCTCTCAGAA372810.2075730737284458No Hit
ACGCGCAGATAC370860.2064873531368027No Hit
CTAACCTCCGCT365540.20352528465088404No Hit
AATCCGTACAGC365270.20337495410742576No Hit
ACAAGGAGGTGA355670.19802986811779813No Hit
TGTAATTGTCGC350740.1952849437502081No Hit
ACGCCACGAATG349300.19448318085176394No Hit
ACTCACGGTATG348630.1941101383920712No Hit
TTCCGTAGGGAT344350.1917271208883622No Hit
GGTCACTGACAG343790.19141532420563392No Hit
GACGGAACCCAT339000.18874834900872597No Hit
ATGTCGAGAGAA338740.18860358626317356No Hit
ATGTGGGACCCA330640.18409366995942525No Hit
GACTTGGTATTC326050.18153805072063453No Hit
TCCCAGAACAAC325510.18123738963371797No Hit
GTTACGCATTAC322930.17980089777400554No Hit
ACGTGAGAGAAT320520.17845905847869276No Hit
ATACTTCGCAGG320200.17828088894570518No Hit
TGTGCTGTGTAG319390.17782989731533033No Hit
GTCGTCCTAAAT315740.17579765107969067No Hit
TACGTCCCGTTC315330.17556937136555031No Hit
TGAGTTCGCTAT309150.17212847225972752No Hit
ATTGGGCTAGGC304910.16976772594764197No Hit
GGTCAGCTTAAC303940.16922764955077335No Hit
GGAAACCACCAC301200.16770207292456715No Hit
AAGTGGACTCTC299740.16688917443031126No Hit
AGCTGGAAGTCC299670.16685019984497024No Hit
ATTAGTTCGCGT296690.16519099606902332No Hit
GATTGGTTGCAC295980.16479568241770712No Hit
CGACTGTCTTAA293150.16321999561034814No Hit
CGTAACCAACCA290720.16186702071922363No Hit
CTGTAGGAGACC286350.1594338930343619No Hit
GCGGCAATTACG285030.1586989437107881No Hit
AAGAGATGTCGA284560.15843725720921256No Hit
GACTTTCCCTCG284410.15835374024062465No Hit
AGCCGGCACATA282840.15747959596940428No Hit
GACACATTTCTG282170.15710655350971153No Hit
CTTCGGCAGAAT281940.15697849415787668No Hit
ATGATGACCCGT279170.1554362141379529No Hit
TAGAACTCACCT275560.15342623909393666No Hit
TAATCGGATTCC267580.1489831363650587No Hit
CCATAGGGTTCA264660.14735733937654694No Hit
TTGCGCATACTA260200.14487410151053245No Hit
TAAGGTAAGGTG259320.14438413529481656No Hit
ATCCCGAATTTG257820.14354896560893726No Hit
GTACGTGGGATC252750.14072609207066517No Hit
GAATGATGAGTG251570.14006909191777342No Hit
TATGCACCAGTG248240.13821501521512133No Hit
CAGGCGTATTGG248090.13813149824653342No Hit
TAACACCACATC244830.13631639612922236No Hit
CGAGAAGAGAAC243460.13555360781611925No Hit
GGAGACAAGGGA241910.1346905991407106No Hit
AATGCCTCAACT237700.13234655622234268No Hit
CCAGTGTATGCA236420.13163387809039231No Hit
GCGACTTGTGTA234460.13054258970084331No Hit
TCTACGGAGAGC231610.1289557672976726No Hit
TGGCATACGGCA229700.12789231789765296No Hit
ATCGGCGTTACA226500.12611062256777708No Hit
GTTGGTCAATCT216880.12075440098233772No Hit
TGCTATATCTGG216580.12058736704516186No Hit
CAGCGGTGACAT216540.1205650958535384No Hit
GGTAACAGCTCG213620.11893929886502667No Hit
GCATGGCTCTAA212660.1184047902660639No Hit
TGAGTCACTGGT210860.11740258664300872No Hit
ATTCATGGACGC207650.11561532351522699No Hit
CAGCTAGAACGC206700.11508638271417009No Hit
ACGATGCGACCA206520.11498616235186457No Hit
CTCCTGAAAGTT205550.11444608595499593No Hit
GTCATTCACGAG199240.11093280547639693No Hit
TGCGGCATCGAA198950.11077133933712693No Hit
GAACCAAAGGAT194540.10831594046064173No Hit
ATTCTGTGAGCG193770.10788722002189036No Hit
AGGCATCTTACG191650.10670684686584758No Hit
TAATCCACAGCG191100.10640061798102517No Hit
TCCGACACAATT191070.10638391458730757No Hit
CCTTAAGTCAGT188450.1049251515359717No Hit
TACACGATCTAC187360.10431826156423274No Hit
TTATCACGTGCA186560.10387283773176377No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATGG600.00339667855.9999952
GCAGCCG1552.7284841E-115.99999485
TCCAAAG121600.05.9926261
CGTGTAC115550.05.99220562
GGGTTTG274300.05.9912452
TCTAGCG64200.05.9906821
TGGCAAG241650.05.9900961
GTCATAT107500.05.9888651
ACACTAG98400.05.98783251
CACTAGA98050.05.9877562
CGGGTTT272550.05.98681931
GAGCCGG113000.05.98672062
CAGGAAG132450.05.98643831
TGAGACT87050.05.98620942
CCATGCG108500.05.9862031
TAAACCG85300.05.985961
ATGAGCT128000.05.9859325
AATGTCC99550.05.98495961
TTAGGGC119200.05.9849271
AGCTGTT113150.05.984121
Produced by FastQC (version 0.11.2)