FastQCFastQC Report
Mon 26 Mar 2018
000000000-BHPG9_l01n01.331000000cc326.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BHPG9_l01n01.331000000cc326.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17960422
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA313186617.43759695624078No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA258896114.414811634158708No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA229974012.804487556027357No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGAGCGTA5409803.012067311113291No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA5074782.8255349456710985No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA4559422.5385929127945883No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA4389552.444012729767708No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA4077522.2702807317110922No Hit
GACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTA3818642.1261415795241336No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATGACTGGGCGTAAAGGGCACGCA3289191.8313545193982637No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA3241471.8047849877914894No Hit
TACGGAGGATGCGAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA2859131.5919058026587571No Hit
TACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA2856261.5903078446597747No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGCGCGTA2754251.5335107382220752No Hit
TACATAGGTCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTTCGTA2624261.4611349332437735No Hit
TACATAGGATGCAAGCGTTATCCGGATTTACTGGGCGTAAAACGAGCGCA2416211.3452968978123119No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA2055411.1444107493688067No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1784900.9937962482173303No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA1160720.6462654385292284No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGAGTA1150370.640502767696661No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA938720.5226603250190892No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA711370.3960764396293138No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTATTGGGCGTAAAGGGAGCGTA604610.3366346291863298No Hit
TACGTAGGTGGCAAGCGTTGTCCGGCATTATTGGGCGTAAAGCGCGCGCA591050.3290846952259808No Hit
TACGTAGGGTGCGAGCGTTAATCGGACTTACTGGGCGTAAAGCGTGCGCA486920.2711072156322385No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCA438560.24418134495948926No Hit
TACGTAGGGTGCGAGCGTTAATCGGCATTACTGGGCGTAAAGCGTGCGCA434680.24202103937201475No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCA404530.22523412868584045No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA356180.1983138258109971No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA351140.1955076556664426No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA322700.1796728384221707No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA306880.17086458213509684No Hit
TACGTAGGTGGCAAGCGTTGTCCGGACTTATTGGGCGTAAAGCGCGCGCA306640.17073095498535612No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGAGAGTGCA298670.16629342005438402No Hit
TACGGAGGGTGCAAGCGTTAATCGGACTTACTGGGCGTAAAGCGCACGCA292710.1629750125024902No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA285230.1588102996689053No Hit
TACGGAGGGTGCAAGCGTTAATCGGCATTACTGGGCGTAAAGCGCACGCA270460.15058666216194697No Hit
TACGGAGGATGCGAGCGTTATCCGGAATCATTGGGCTTAAAGGGTCCGTA261590.14564802541944727No Hit
GACGGGGGGGGCAAGTGTTCTTCGGAATGACTGGGCGTAAAGGGCACGTA251200.13986308339525652No Hit
TACATAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA247580.13784754055333445No Hit
TACAGAGGATGCAAGCGTTATCCGGAATGATTGGGCGTAAAGCGTCTGTA234070.13032544558251472No Hit
TACGGAGGATACGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA231710.12901144527673125No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA230570.12837671631546296No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGCGCACGCA230050.12808719082435813No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA227980.12693465665784467No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA227030.12640571585678775No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA216280.120420333107986No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGTA212480.11830456990375839No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATGACTGGGCGTAAAGCGCACGCA199280.11095507666802039No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA198380.1104539748564928No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA197780.11011990698214105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAG4961900.0244.891399
TACGGAG4740300.0244.863041
AGGATGC1157650.0244.746056
ACGGAGG4738400.0244.64842
ATAGGAT265350.0244.630684
ACATAGG633650.0244.613362
TACGTAG10706850.0244.581251
GGATGCA746250.0244.540377
TACATAG633950.0244.439641
ACGTATG204850.0244.402012
TACGTAT205600.0244.344591
GTAGGGT3974000.0244.315674
GGTCCCG189800.0244.290047
TAGGGTG3970150.0244.210115
AGGTCCC192500.0244.109096
TAGGTCC192550.0243.854865
CAAATAG234350.0243.37955245
GTGGCAA4757850.0243.318748
CATAGGT322900.0243.254873
ACGTAGG10745450.0243.212462
Produced by FastQC (version 0.11.2)