FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n02_mini_barcodes11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n02_mini_barcodes11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398260
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAT5100.0145.000026
GGAGCAA450.0145.000025
CTCGTCA450.0145.000024
CCCTATC450.0145.000023
ATGGCCA550.0145.000024
CCTAGCA450.0145.000024
GGCACCA450.0145.000024
TGAACCA450.0145.000024
GGCTTCA550.0145.000024
CGCCTCA450.0145.000024
GAGACAA550.0145.000025
GGGACCA450.0145.000024
AGGCCAA1050.0145.000025
GCTTACA450.0145.000024
GAAACCA450.0145.000024
CTCGCCA550.0145.000024
CCCCAAT10200.0145.000026
ACGTCCA550.0145.000024
ACACTCA550.0145.000024
ACGTACA550.0145.000024