FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n02_mini_barcodes10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n02_mini_barcodes10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308286
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCT1650.0145.000025
CAGCGCT1450.0145.000025
GTCGCTT5750.0145.000026
CCCAGCT1650.0145.000025
CCATGCT1650.0145.000025
TCGTGCT1450.0145.000025
CTCGGCT1450.0145.000025
TACAGCT1650.0145.000025
AGGTCAG100.0070885005145.03
CGGACAG100.0070885005145.03
TCCGGCC100.0070885005145.02
AGCACCA100.0070885005145.01
TCCGGAC100.0070885005145.01
TCACGCC100.0070885005145.02
TCTGCGC400.0145.04
CTGCTTG202.128245E-6145.03
CTTGTAC100.0070885005145.02
TCGCCGC650.0145.04
GTGCATA100.0070885005145.01
GCCCCGG202.128245E-6145.03
Produced by FastQC (version 0.11.2)