FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n02_mini_barcodes09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n02_mini_barcodes09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287519
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATGA1800.0145.000024
TGGAGTG450.0145.000023
GAGGAGA450.0145.000024
CAGAAGA450.0145.000024
CAGCGGA900.0145.000024
TCGCATC450.0145.000022
ACAAATG450.0145.000023
CTCGTTG450.0145.000023
CGACGGA900.0145.000024
CCGATCG450.0145.000023
ATAATTG450.0145.000023
CGTGAGA900.0145.000024
CGTTGGA1050.0145.000024
TGTGGAT3600.0145.000025
GGTCAAG450.0145.000023
AATATTG450.0145.000023
AGACGGA450.0145.000024
GGAAGGA450.0145.000024
ACGATGA1050.0145.000024
GGGACGA900.0145.000024