FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n02_mini_barcodes08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n02_mini_barcodes08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780736
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCCT2500.0145.000025
GGGCCTG5050.0145.000026
CTGGCCT2500.0145.000025
CTTGTTC253.7107384E-8145.03
AGGTCAT151.2299028E-4145.02
GCATAAG100.0070905657145.01
TCAAAAA100.0070905657145.02
GCGAGAG100.0070905657145.01
CGGACAG100.0070905657145.01
TCACGGA100.0070905657145.01
ACGCGAC100.0070905657145.01
TGATCAT100.0070905657145.02
CTTAATG151.2299028E-4145.02
CTGCTTC400.0145.03
TCTGCCA100.0070905657145.02
GCCCCTG13700.0145.06
CCTTCAT202.1303113E-6145.01
CCTTCAA100.0070905657145.01
TCTGCAG100.0070905657145.01
ACAACTA100.0070905657145.02
Produced by FastQC (version 0.11.2)