FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n02_mini_barcodes07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n02_mini_barcodes07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109166
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTTG550.0145.000024
GCAACTG1450.0145.000024
CCGGATG1100.0145.000024
ATTAACT550.0145.000023
GGCCATG1450.0145.000024
CCCGTTG1100.0145.000024
ACGTGTG550.0145.000024
CGAGCTG550.0145.000024
TCGAGTG550.0145.000024
GCAGCTG550.0145.000024
GGATTTG550.0145.000024
CGACTTG1100.0145.000024
CGAAGTG1100.0145.000024
CCCTGAA151.2300414E-4145.01
CCTTCGG253.711648E-8145.02
CCTTCAA151.2300414E-4145.01
ATCCTTG1900.0145.04
GTGCATG750.0145.04
GTTGACC151.2300414E-4145.01
AACGTTA151.2300414E-4145.02
Produced by FastQC (version 0.11.2)