FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n02_mini_barcodes06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n02_mini_barcodes06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705648
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCACT57550.0145.000027
GCGCACT34850.0145.000027
TAGGGCA1650.0145.000025
CTTGTTG151.229853E-4145.03
CTTGTTC100.0070904233145.02
AGGTCAT100.0070904233145.01
TAATCAG151.229853E-4145.03
GCATAAG100.0070904233145.03
TAATCAC151.229853E-4145.02
AGGTCAA100.0070904233145.01
GGGGCAG151.229853E-4145.03
TCACGGG253.7103746E-8145.03
GGGGCAC4300.0145.06
CGGACAG100.0070904233145.01
CGGACAA253.7103746E-8145.01
AGCACCT100.0070904233145.02
TGATCCC151.229853E-4145.02
CCCTGAC151.229853E-4145.02
CCCTGAA100.0070904233145.01
TCCGGAC100.0070904233145.02
Produced by FastQC (version 0.11.2)