FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n02_mini_barcodes05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n02_mini_barcodes05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100778
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGCG550.0145.000025
CAATGCG550.0145.000025
GGCCGCG550.0145.000025
TAACGCG550.0145.000025
TGCTGCG550.0145.000025
CCTTGCG550.0145.000025
ACTAGCG550.0145.000025
AGCTGCG550.0145.000025
ACGTGCG550.0145.000025
GCCCTCG151.2267502E-4145.03
TTTTGTG100.007081469145.03
TGAGACG100.007081469145.03
CAGCCCA100.007081469145.02
CTTTTGC100.007081469145.04
CTTCATC100.007081469145.02
GCCCTAT100.007081469145.02
ACTGCTG100.007081469145.03
CTCGACG100.007081469145.03
CTTTCCG151.2267502E-4145.03
CTCTGGG100.007081469145.03
Produced by FastQC (version 0.11.2)