FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n02_mini_barcodes04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n02_mini_barcodes04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177867
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGTG100.0070859953145.000023
GCCCCAG100.0070859953145.000023
CAGCCCG100.0070859953145.000023
GCCCTAC100.0070859953145.000021
CTACAGG100.0070859953145.000023
CTTTCCG100.0070859953145.000023
ATCCTGT400.0145.000024
TAGAAGT100.0070859953145.000024
AGCCTGT202.125742E-6145.000024
TTGTCTG100.0070859953145.000023
CTTTTCG100.0070859953145.000021
TTATGCT100.0070859953145.000021
CGTTCAC100.0070859953145.000021
TTGTTTG100.0070859953145.000023
ATCCCCG100.0070859953145.000023
CTACACA100.0070859953145.000021
TGGTTTA100.0070859953145.000022
CGTTTAC100.0070859953145.000022
ATCCTCG100.0070859953145.000023
ATCCCAT100.0070859953145.000021
Produced by FastQC (version 0.11.2)