FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n02_mini_barcodes03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n02_mini_barcodes03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1534201
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGCC100.0070912284145.01
TTGGCGT120100.0145.07
CCGGGCC202.1309734E-6145.02
ATGGCCC151.2301326E-4145.02
GGGTCTA151.2301326E-4145.02
GCTGGGC2700.0145.05
CGTGCAA100.0070912284145.02
TGGACCT151.2301326E-4145.01
GTCCTGC253.7123755E-8145.01
GGCCGCT100.0070912284145.01
CGCTGCT100.0070912284145.01
GCTTGCC100.0070912284145.02
GCCGCCA100.0070912284145.02
GTCAGTA100.0070912284145.01
GACCAGT100.0070912284145.02
GGAAGCC100.0070912284145.02
AGGCCCT100.0070912284145.01
TGCGCCA100.0070912284145.02
TGCGACC100.0070912284145.02
CAGGCCG306.511982E-10145.03
Produced by FastQC (version 0.11.2)