FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n02_mini_barcodes02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n02_mini_barcodes02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859899
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCAGG4100.0145.000025
TTCAAGG7300.0145.000025
CCTCCAG2050.0145.000024
CGAGGGT37350.0145.000027
TGTAAGG2850.0145.000025
CCCGAGG3650.0145.000025
TAATCAT151.2299458E-4145.02
TCACGGG100.0070906905145.06
TGATCCT100.0070906905145.02
AGCACAG900.0145.04
TGATCAG800.0145.04
TCTGCGT100.0070906905145.01
CCTTCGC151.2299458E-4145.02
CTTAATT100.0070906905145.02
GTTGATA100.0070906905145.03
CTGCTTT202.130435E-6145.01
GCCCCTG100.0070906905145.02
GTCATTT100.0070906905145.02
CTTGTCG100.0070906905145.01
TGTTTTG151.2299458E-4145.02
Produced by FastQC (version 0.11.2)