FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n02_mini_barcodes01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n02_mini_barcodes01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1835502
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGACT1050.0145.000024
CCCTGAT100.0070913415145.02
CTGCTGA306.511982E-10145.02
CGGGTGT202.1310861E-6145.01
GACAGTG100.0070913415145.02
CGTGGGC151.2301716E-4145.03
CGTGGGA100.0070913415145.02
CTATGCG100.0070913415145.02
CGTGGCG100.0070913415145.01
AGACTCG100.0070913415145.01
GGGGACT700.0145.04
GACAGGA100.0070913415145.02
CGGCGCA202.1310861E-6145.02
GGAGCTA100.0070913415145.02
ACCGGAC253.7127393E-8145.03
ACTGCCG100.0070913415145.01
CGTATGC202.1310861E-6145.03
GAGCTAT100.0070913415145.02
CTCCGGA100.0070913415145.02
GGTCGGC100.0070913415145.03
Produced by FastQC (version 0.11.2)