FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n01_mini_barcodes12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n01_mini_barcodes12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6098
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGCA100.0069188206145.06
AAACCGC100.0069188206145.05
AACGCAT201.962384E-6145.07
TTCACCG151.17091935E-4145.04
AATCCGC100.0069188206145.05
ACTCGCA100.0069188206145.06
AACCGCA201.962384E-6145.06
TTCGCAT600.0145.07
TGCGCAT450.0145.07
CCTGTTC100.0069188206145.03
ACGCATA1350.0145.08
ACGTCCG100.0069188206145.04
CCTGCGC151.17091935E-4145.05
GTCGCAT100.0069188206145.07
TTCCGCA100.0069188206145.06
GGCGCAT100.0069188206145.07
TGCCGCA100.0069188206145.06
CACGCGC100.0069188206145.05
GTCCGCA151.17091935E-4145.06
CACCGCA305.475158E-10145.06