FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n01_mini_barcodes11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n01_mini_barcodes11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398260
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTGA450.0145.000024
ATCCTGA550.0145.000024
GGAATCG70400.0145.000028
GGCAGAA1100.0145.000025
GTTCTGA550.0145.000024
CAGTTGA450.0145.000024
GTGGGAA450.0145.000025
CGCGCGA550.0145.000024
CCGCTGA450.0145.000024
GCCATGA450.0145.000024
GCTTGGA450.0145.000024
TCTAGGA450.0145.000024
TCCTAGA450.0145.000024
TGGCCGA450.0145.000024
TCAGGGA550.0145.000024
GTCAGGA550.0145.000024
ATCCGGA550.0145.000024
ATTCCGA900.0145.000024
AGACGAA1050.0145.000025
GCCCTGA900.0145.000024