FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n01_mini_barcodes10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n01_mini_barcodes10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308286
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTC1450.0145.000025
TACGTTC1450.0145.000025
TCCGGCT253.7052814E-8145.03
CCCTGCG100.0070885005145.01
CTGCTTC1600.0145.05
GCCCCTC100.0070885005145.01
TCTGCAC100.0070885005145.02
TTTAGTC100.0070885005145.01
GCCCCGT100.0070885005145.03
GCCCCGC100.0070885005145.02
TCGCCAT151.2291866E-4145.03
TTCTATG100.0070885005145.01
TCGCCAC100.0070885005145.02
CTGCTCT100.0070885005145.03
GTCATGT151.2291866E-4145.03
GCCCCCG100.0070885005145.01
CATGGTC100.0070885005145.02
GCCCCCC100.0070885005145.01
GCCCCAC100.0070885005145.01
ATCCTCA151.2291866E-4145.02
Produced by FastQC (version 0.11.2)