FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n01_mini_barcodes09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n01_mini_barcodes09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287519
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTAG1050.0145.000024
TGGAGAG450.0145.000024
GGAGCAG450.0145.000024
CGGCCTA450.0145.000023
TGGGTAG450.0145.000024
GGCGTAG450.0145.000024
TGTGGAG450.0145.000024
ACATTAG1800.0145.000024
GCTTGAG900.0145.000024
CTGGTCA450.0145.000023
GCCGCAG1050.0145.000024
GACCATA450.0145.000023
TAGCAAG900.0145.000024
GCATTAG1800.0145.000024
GGGCGAG450.0145.000024
ACCAGAG1050.0145.000024
CGGGAGT1800.0145.000025
CGGGAAG450.0145.000024
ATGGTAG450.0145.000024
ACCAATA450.0145.000023