FastQCFastQC Report
Tue 31 Oct 2017
000000000-BH5K9_l01n01_mini_barcodes08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BH5K9_l01n01_mini_barcodes08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences780736
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAGCT450.0145.000024
GCGAACT450.0145.000024
GCAACAC450.0145.000023
GTTAGCT900.0145.000024
GGATACT450.0145.000024
CTGACAC450.0145.000023
TCGGACT450.0145.000024
TGCGCCT450.0145.000024
CTCGCGC450.0145.000023
TCCCGCC450.0145.000023
CTTGTTT100.0070905657145.01
ACGCGAT100.0070905657145.02
GGGGCAG100.0070905657145.02
TCACGGG100.0070905657145.01
GGGGCAC100.0070905657145.03
CGGACAG100.0070905657145.01
ACGCGAC306.511982E-10145.03
CCCTGCA100.0070905657145.01
TCCGGCG100.0070905657145.01
CCCTGAG202.1303113E-6145.02
Produced by FastQC (version 0.11.2)